Preparation of Controlled Multicompartmental Gel Microcarriers Based on Aqueous Two-Phase Emulsions for 3D Partitioned Cell Coculture In Vitro DOI

Huatao He,

Meiying Hong,

Feng Yang

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(7), P. 4469 - 4481

Published: June 15, 2024

A facile method was proposed for preparing controllable multicompartment gel microcarriers using an aqueous two-phase emulsion system. By leveraging the density difference between upper polyethylene glycol solution and lower dextran-calcium chloride (CaCl2) in collection high viscosity of solution, fusion core–shell droplets made by coextrusion devices achieved at water/water (w/w) interface to fabricate with separated core compartments. adjusting sodium alginate concentration, collected composition, number fused liquid droplets, pore size, shape, compartments could be controlled. Caco-2 HepG2 cells were encapsulated different establish gut–liver coculture models, exhibiting higher viability proliferation compared monoculture models. Notably, significant differences cytokine expression functional proteins observed This provides new possibilities complex three-dimensional materials.

Language: Английский

One‐Step Fabrication of Highly Customizable Porous Core–Shell Microspheres for the Construction of a 3D Physiologically Relevant Perivascular‐Endosteal Multi‐Microenvironment Model of Breast Cancer Bone Metastasis DOI Open Access

Meiying Hong,

Guanxiong Wang,

Menghan Yang

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Abstract The 3D model of the perivascular‐endosteal multi‐microenvironment (PVM‐EM) is crucial for studying early stage breast cancer (BrCa) bone metastasis. However, existing models struggle to accurately represent composition and spatial intricacies multi‐microenvironments, limiting their ability support cellular functional expression within these systems. Here, nested aqueous two‐phase system emulsion as a template develop novel type core‐shell microsphere establishing PVM‐EM utilized. This enables precise control over chemical composition, macroporous structure, cell localization. By adjusting parameters in each microenvironment, it successfully reconstructed PVM with compartmentalized distribution core EM shell one step. outcomes indicate that porous architecture localization significantly enhance activity function microenvironment. Importantly, this effectively encapsulates key biological processes associated colonization BrCa metastasis, including elevated cytokine expression, extensive angiogenesis PVM, significant inhibition alkaline phosphatase EM. method paves way efficient physiologically relevant models, facilitating future preclinical research drug screening

Language: Английский

Citations

1
Recent advances of macroporous hydrogel microparticles: Fabrication and applications DOI
Yingzhe Liu,

Sida Ling,

Zhuo Chen

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 161435 - 161435

Published: March 1, 2025

Language: Английский

Citations

0
Preparation of Controlled Multicompartmental Gel Microcarriers Based on Aqueous Two-Phase Emulsions for 3D Partitioned Cell Coculture In Vitro DOI

Huatao He,

Meiying Hong,

Feng Yang

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(7), P. 4469 - 4481

Published: June 15, 2024

A facile method was proposed for preparing controllable multicompartment gel microcarriers using an aqueous two-phase emulsion system. By leveraging the density difference between upper polyethylene glycol solution and lower dextran-calcium chloride (CaCl2) in collection high viscosity of solution, fusion core–shell droplets made by coextrusion devices achieved at water/water (w/w) interface to fabricate with separated core compartments. adjusting sodium alginate concentration, collected composition, number fused liquid droplets, pore size, shape, compartments could be controlled. Caco-2 HepG2 cells were encapsulated different establish gut–liver coculture models, exhibiting higher viability proliferation compared monoculture models. Notably, significant differences cytokine expression functional proteins observed This provides new possibilities complex three-dimensional materials.

Language: Английский

Citations

2