Published: Jan. 1, 2024
Language: Английский
Advanced Science, Journal Year: 2024, Volume and Issue: 11(32)
Published: June 26, 2024
Here, a separation-free and label-free portable aptasensor is developed for rapid sensitive analysis of tumor-derived exosomes (TEXs). It integrated parallel rolling circle amplification (RCA) reaction, selective binding metal ions or small molecules to nucleic acid-specific conformations, low-cost, highly handheld fluorometer. Lung cancer, example, targeted with two typical biomarkers (mucin 1 programmed cell death ligand (PD-L1)) on its exosomes. The affinity aptamers the targets modulated amount RCA products (T-Hg
Language: Английский
Citations
11
Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 275, P. 117236 - 117236
Published: Feb. 6, 2025
Language: Английский
Citations
1
Small Methods, Journal Year: 2025, Volume and Issue: unknown
Published: March 22, 2025
Abstract An ultrasensitive ICP‐MS aptasensor is developed utilizing a label‐free, simple filter membrane‐assisted separation technique combined with nucleic acid signal amplification for the analysis of circulating tumor cells (CTCs) in lung cancer clinical samples. The approach based on high‐affinity interaction between aptamers and PD‐L1 mucin 1, which are overexpressed cell surface, conjunction functional Y‐DNA nanospheres catalytic hairpin assembly amplifications, enabling simultaneous detection two proteins. Additionally, four‐armed nanostructure significant spatial site resistance self‐assembled by introducing streptavidin biotinylated‐hairpin structures, improving efficiency membrane. This structural design enables effective isolation biotin‐T‐Hg 2+ ‐T biotin‐C‐Ag + ‐C from free Hg Ag , facilitating highly sensitive dual‐protein via ICP‐MS. limits reached ag mL −1 levels proteins single‐cell A549 cells. CTCs extracted whole blood samples patients within 45 min through centrifugation procedure. Quantification performed 37 samples, demonstrating results consistent diagnoses. assay exhibits specificity 100% sensitivity 94.5%.
Language: Английский
Citations
1
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Herein, a supramolecular DNA nanodevice was formed via the rolling circle amplification (RCA) and hybridization chain reaction (HCR) cascade on tetrahedral nanostructure (TDN) to achieve simultaneous sensitive detection intracellular imaging of dual-miRNAs related liver cancer. The effectively addressed limitations low probe loading capacity in traditional TDN nanodevices by enriching plenty signal probes around single TDN, significantly enhancing fluorescence signal. Impressively, with fulcrum dense structure imparted strong rigidity, ensuring stability decrease aggregation quenching for further increasing response. Consequently, biosensor based enabled miRNA221 miRNA222, achieved accurate situ displaying improved capabilities compared TDN-based nanodevices. More importantly, precise miRNA222 could distinguish hepatocellular carcinoma cells different degrees metastasis from human normal cells, providing more information diagnosis development carcinoma.
Language: Английский
Citations
0
ACS Sensors, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 19, 2025
Fluorescence sensing is widely used in vitro detection due to its high sensitivity and rapid result delivery. However, systems based on nanomaterials involving complex cumbersome purification steps can lead sample loss significantly reduce the accuracy of results. To address this issue, we proposed a lanthanide-based aptasensor featuring target-triggered antenna effect enhance time-resolved luminescence (TRL) chelated Tb3+ combined with wash-free strategy. This sensor enabled highly sensitive epithelial cell adhesion molecule (EpCAM) exosomes from tumors, eliminating necessity for thereby delivering more stable reliable Specifically, using computer-aided design, sequence capable self-folding into hairpin structure (Antenna-Hairpin-Tb) was obtained. After hybridization an EpCAM-specific aptamer (AptEpCAM), duplex (Probe-Tb) constructed detection. When targeted binding occurred between AptEpCAM exosomes, Antenna-Hairpin-Tb dehybridized Probe-Tb underwent self-folding, leading significant sensitized TRL signal effect-induced energy transfer ligand Tb3+. Over broad range exosome concentrations (spanning 6.19 × 102 108 particles/mL), emission intensity at 543 nm linearly increased concentration (linear relationship: Y = 332.62 lg(Nexosomes) + 3690.5, R2 0.9956), achieving theoretical limit as low 17 particles/mL buffer. Furthermore, swift effective differentiation all cancer serum samples nonepithelial ones within just 30 min operation, 100% sensitivity, specificity, accuracy.
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 18, 2025
Circular tumor DNA (ctDNA) is a trace nucleic acid that functions as an essential marker. In this context, the present study proposes one-pot electrochemical analysis of ctDNA EGFR L858R in lung cancer leveraging Ag+-mediated nanosphere (I amplification) and cation exchange reaction (II amplification), Cu2+ acts signal molecule. Once target exists, it specifically destroys structure nanosphere@Ag+, large amounts Ag+ are released. After addition copper sulfide nanoparticles, can be replaced by reaction. Eventually, elevated. The analytical performance method satisfactory, detected linear range 1 aM-1 fM with detection limit 0.3 aM. Furthermore, system exhibits notable selectivity differentiating base mismatch targets other sequences. recovery rate blood samples between 95.5 105%. results from 42 clinical consistent those quantitative real-time polymerase chain reaction, computed tomography, pathology results. summary, novel strategy utilizes preprepared functional nanomaterials cascade amplification, which expected to contribute sensitive expeditious acids.
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 30, 2025
The highly sensitive and rapid imaging of miRNA in living cells promises to advance our understanding diseases promote their diagnosis treatment. To enhance the sensitivity imaging, a series cascade signal amplification strategies based on enzyme-free methods have been developed. However, these cascaded involve complex designs multiple mechanisms, leading potential side effects. Herein, we developed novel hairpins@ZIF-8 nanosystem by rationally integrating ZIF-8 with self-feedback DNAzyme circuit (CSDC), achieving cells. facilitates efficient transfection nucleic acid probes into provides necessary cofactor ions for CSDC. CSDC possesses exponential solely mechanism. Benefiting from efficiency, exhibited higher than traditional DNAzyme-based amplification, detection limit 2.28 fM, approximately 105 times more amplification. This demonstrated strong practical application capabilities, effectively reflecting fluctuations intracellular levels successfully distinguishing between normal tumor expression differences. It could also be applied vivo imaging. proposed strategy is anticipated pave way innovative approaches serve as vital instrument miRNA-related research, diagnosis,
Language: Английский
Citations
0
Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137372 - 137372
Published: Feb. 1, 2025
Language: Английский
Citations
0
Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117362 - 117362
Published: March 1, 2025
Language: Английский
Citations
0
Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162192 - 162192
Published: March 1, 2025
Language: Английский
Citations
0