Bicistronic Vector Expression of Recombinant Jararhagin-C and Its Effects on Endothelial Cells DOI Creative Commons
Karla Fernanda Ferraz, Lhiri Hanna De Lucca Caetano,

Daniele Pereira Orefice

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(12), P. 524 - 524

Published: Dec. 3, 2024

Jararhagin-C (JarC) is a protein from the venom of Bothrops jararaca consisting disintegrin-like and cysteine-rich domains. JarC shows modulating effect on angiogenesis remodeling extracellular matrix constituents, improving wound healing in mouse experimental model. purified crude venom, yield less than 1%. The aim this work was to obtain recombinant form test its biological activity. For purpose, bicistronic vector pSUMOUlp1 used. This allowed expression toxin (rJarC) fusion with small ubiquitin-related modifier (SUMO) as well SUMO protease Ulp1. After expression, able efficiently remove rJarC inside bacteria. free at expected molecular mass recognized by polyclonal anti-jararhagin antibodies. In terms activity, both native forms showed no toxicity HUVEC cell line CRL1730 were effective migration activity vitro These results demonstrate successful production preservation which may facilitate further investigations into therapeutic potential snake venom-derived protein.

Language: Английский

A novel IgG-Fc-Fused multiepitope vaccine against Brucella: robust immunogenicity DOI Creative Commons

Aodi Wu,

Yuting Zhang,

Caidong Liu

et al.

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: April 15, 2025

Brucellosis is one of the most common zoonotic diseases caused by Brucella spp. However, there currently no vaccine available for humans. Although some attenuated live vaccines have been approved animals, their protective efficacy suboptimal. In previous studies, we utilized an epitope- and structure-based vaccinology platform to identify immunodominant epitopes antigens OMP19, OMP16, OMP25, L7/L12, constructed multi-epitope MEV-Fc against Brucella. this study, was expressed purified via Escherichia coli expression system, which validated that possesses high immunological exerts a significant effect in BALB/c mice within infection model. enhanced Th1 Th2 immune responses strongly induced production pro-inflammatory cytokine IFN-γ. Furthermore, protected compared control group (PBS). conclusion, our results provide new insights data support development human vaccines.

Language: Английский

Citations

0
Development of Complex Generics and Similar Biological Products: An Industrial Perspective of Reverse Engineering DOI
Rajeev Ranjan

AAPS PharmSciTech, Journal Year: 2025, Volume and Issue: 26(4)

Published: March 26, 2025

Language: Английский

Citations

0
The response surface method enables efficient optimization of induction parameters for the production of bioactive peptides in fed-batch bioreactors using Escherichia coli DOI
Z.R. Khasanshina, I. A. Kornakov, E. A. Buslaeva

et al.

Folia Microbiologica, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Language: Английский

Citations

0
Bicistronic Vector Expression of Recombinant Jararhagin-C and Its Effects on Endothelial Cells DOI Creative Commons
Karla Fernanda Ferraz, Lhiri Hanna De Lucca Caetano,

Daniele Pereira Orefice

et al.

Toxins, Journal Year: 2024, Volume and Issue: 16(12), P. 524 - 524

Published: Dec. 3, 2024

Jararhagin-C (JarC) is a protein from the venom of Bothrops jararaca consisting disintegrin-like and cysteine-rich domains. JarC shows modulating effect on angiogenesis remodeling extracellular matrix constituents, improving wound healing in mouse experimental model. purified crude venom, yield less than 1%. The aim this work was to obtain recombinant form test its biological activity. For purpose, bicistronic vector pSUMOUlp1 used. This allowed expression toxin (rJarC) fusion with small ubiquitin-related modifier (SUMO) as well SUMO protease Ulp1. After expression, able efficiently remove rJarC inside bacteria. free at expected molecular mass recognized by polyclonal anti-jararhagin antibodies. In terms activity, both native forms showed no toxicity HUVEC cell line CRL1730 were effective migration activity vitro These results demonstrate successful production preservation which may facilitate further investigations into therapeutic potential snake venom-derived protein.

Language: Английский

Citations

0