Comparison of Lung Tissue‐Derived Extracellular Vesicles Using Multiple Dissociation Methods for Profiling Protein Biomarkers DOI Creative Commons
Yue Han, Meng Ye, Sheng Ye

et al.

Biotechnology Journal, Journal Year: 2024, Volume and Issue: 19(9)

Published: Sept. 1, 2024

ABSTRACT Extracellular vesicles (EVs) operate as chemical messengers that facilitate intercellular communication. Emerging evidence has demonstrated lung tissue‐derived EVs play pivotal roles in pulmonary physiological processes and have potential biomarkers therapeutics for diseases. Multiple methods been proposed the isolation of EVs. However, effects different tissue pre‐treatments on EV subsequent disease biomarker discovery not yet comprehensively investigated. In this study, we compared physical characteristics, recovery yields, protein compositions isolated from tissues using three based dissociation principles. Methodologically, beneficial blood perfusion gentle meshing were emphasized their impact yield purity. These results demonstrate enrich distinct subpopulations exhibit significant differences cargo surface properties. disparities directly affect diagnostic detection marker proteins related to diseases, including tumors, asthma, fibrosis. Collectively, these findings highlight variations characteristics resulting applied approaches offer compelling suggestions guiding researchers selecting a suitable method downstream functional studies clinical applications.

Language: Английский

Enigmatic exosomal connection in lung cancer drug resistance DOI Creative Commons

Sambit K. Patra,

Rajeev Kumar Sahoo,

Stuti Biswal

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2024, Volume and Issue: 35(2), P. 102177 - 102177

Published: March 22, 2024

Lung cancer remains a significant global health concern with limited treatment options and poor prognosis, particularly in advanced stages. Small extracellular vesicles such as exosomes, secreted by cells, play pivotal role mediating drug resistance lung cancer. Exosomes have been found to facilitate intercellular communication transferring various biomolecules between cells their microenvironment. Additionally, exosomes can transport signaling molecules promoting cell survival proliferation conferring chemotherapy. Moreover, modulate the tumor microenvironment inducing phenotypic changes hindering response. Understanding of is crucial for developing novel therapeutic strategies biomarkers overcome limitations. In this review, we summarize current knowledge on conventional emerging mechanisms involvement well exosome-mediated factors cancer.Graphical abstract

Language: Английский

Citations

6
An updated review on the development of a nanomaterial-based field-effect transistor-type biosensors to detect exosomes for cancer diagnosis DOI
Jeongyun An, Hyunjun Park,

Minyoung Ju

et al.

Talanta, Journal Year: 2024, Volume and Issue: 279, P. 126604 - 126604

Published: July 22, 2024

Language: Английский

Citations

4
Exosome transmit the ability of migration and invasion in heterogeneous ovarian cancer cells by regulating autophagy via targeting hsa-miR-328 DOI
Hengzi Sun, Xiao Huo,

Xiaoning Bi

et al.

Gynecologic Oncology, Journal Year: 2025, Volume and Issue: 194, P. 60 - 70

Published: Feb. 18, 2025

Language: Английский

Citations

0
Extracellular vesicles as nature's nano carriers in cancer therapy: insights toward preclinical studies and clinical applications DOI Creative Commons
Xiaotong Wu,

Yuhua Meng,

Zhimeng Yao

et al.

Pharmacological Research, Journal Year: 2025, Volume and Issue: unknown, P. 107751 - 107751

Published: May 1, 2025

Extracellular vesicles (EVs), which are secreted by various cell types, hold significant potential for cancer therapy. However, there several challenges and difficulties that limit their application in clinical settings. This review, integrates the work of our team recent advancements this research field, discusses EV-based treatment strategies to guide application. The following discussed: 1) leveraging inherent properties EVs development treatments; 2) modifying using EV engineering methods improve drug loading delivery; 3) targeting key molecules tumor-derived (TDE) synthesis inhibit production; 4) clearing TDEs from tumor microenvironment. Additionally, on basis into vaccines bispecific antibodies, review elaborates enhance antitumor immunity via modifications can ability stability progress AI technology targeted delivery drugs. Although limited enhancing abilities, provides an in-depth discussion prior studies. Finally, summarizes use therapy highlights need be addressed.

Language: Английский

Citations

0
Exosome Transmit the Ability of Migration and Invasion in Heterogeneous Ovarian Cancer Cells by Regulating Autophagy Via Targeting hsa-miR-328 DOI
Hengzi Sun, Xiao Huo,

Xiaoning Bi

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Comparison of Lung Tissue‐Derived Extracellular Vesicles Using Multiple Dissociation Methods for Profiling Protein Biomarkers DOI Creative Commons
Yue Han, Meng Ye, Sheng Ye

et al.

Biotechnology Journal, Journal Year: 2024, Volume and Issue: 19(9)

Published: Sept. 1, 2024

ABSTRACT Extracellular vesicles (EVs) operate as chemical messengers that facilitate intercellular communication. Emerging evidence has demonstrated lung tissue‐derived EVs play pivotal roles in pulmonary physiological processes and have potential biomarkers therapeutics for diseases. Multiple methods been proposed the isolation of EVs. However, effects different tissue pre‐treatments on EV subsequent disease biomarker discovery not yet comprehensively investigated. In this study, we compared physical characteristics, recovery yields, protein compositions isolated from tissues using three based dissociation principles. Methodologically, beneficial blood perfusion gentle meshing were emphasized their impact yield purity. These results demonstrate enrich distinct subpopulations exhibit significant differences cargo surface properties. disparities directly affect diagnostic detection marker proteins related to diseases, including tumors, asthma, fibrosis. Collectively, these findings highlight variations characteristics resulting applied approaches offer compelling suggestions guiding researchers selecting a suitable method downstream functional studies clinical applications.

Language: Английский

Citations

0