Combined cross-linking of Rhizomucor miehei lipase and Candida antarctica lipase B for the effective enrichment of omega-3 fatty acids in fish oil

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 260, P. 129362 - 129362

Published: Jan. 23, 2024

Language: Английский

---

Assessing the Industrial Edge of the Lipase-Mediated Oxidation of 2,5-Diformylfuran to 2,5-Furandicarboxylic Acid: Rotating Bed Reactors, an “Acyl-Donor-Free” Oxidation Concept, and Environmental Aspects

Organic Process Research & Development, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Language: Английский

---

Cyclohexene oxide CA, a derivative of zeylenone, exhibits anti-cancer activity in glioblastoma by inducing G0/G1 phase arrest through interference with EZH2

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 9, 2024

Introduction: Due to its highly aggressiveness and malignancy, glioblastoma (GBM) urgently requires a safe effective treatment strategy. Zeylenone, natural polyoxygenated cyclohexenes compound isolated from Uvaria grandiflora , has exhibited potential biological activities in various human diseases, including tumors. Methods: We designed synthesized series of (+)-Zeylenone analogues evaluated their anti-GBM roles through structural-activity analysis. Cell Counting Kit-8, TUNEL, transwell flow cytometry were employed for investigating the anticancer effects CA on GBM cells. Western blotting, molecular docking, qRT-PCR ChIP assays performed reveal underlying mechanisms by which regulates cell cycle. The nude mouse xenograft model, HE staining, immunohistochemistry was used evaluate effect vivo . Results: identified ((1R, 2R, 3S)-3-p-fluorobenzoyl-zeylenone) as having lowest IC 50 value significantly inhibited malignant behaviors cells induced G0/G1 phase arrest vitro Furthermore, we validated mechanism interferes with EZH2, attenuating down-regulation cyclin-dependent kinase inhibitors p27 p16 PRC2 complex. By establishing orthotopic mice models, further inhibitory role tumorigenesis values synergistically potentiate anti-tumor EZH2 inhibitors. Conclusion: Overall, this paper elucidated CA, may provide therapeutic drug candidate treatment.

Language: Английский

---

Metal Affinity Fusion Enzyme Immobilization: Batch Process Showcase for Cascading Alcohol Oxidation and Bayer–Villiger Oxidation in Microaqueous Media

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(29), P. 10820 - 10830

Published: July 8, 2024

Language: Английский

---