Short‐term impact of vitamin K2 supplementation on biochemical parameters and lipoprotein fractions DOI
Miloš Barna, Kateřina Dunovská,

Jana Čepová

et al.

Electrophoresis, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 2, 2024

This study explored the short-term effects of vitamin K2 (VK2) supplementation on biochemical parameters (vitamin D, E, A, alkaline phosphatase, calcium, phosphorus (P), magnesium, metallothionein, triglycerides, cholesterol, high-density lipoprotein (HDL), low-density (LDL), and fractions (albumin, HDL, very (VLDL), LDL, chylomicrons). A experiment (24 h, six probands) was performed to track changes in VK2 levels after a single-dose intake (360 µg/day). Liquid chromatography-tandem mass spectrometry used monitor K (menaquinone-4 (MK-4), menaquinone-7 (MK-7), K1 [VK1]) with limit detection 1.9 pg/mL for VK1 3.8 two forms VK2. Results showed that MK-7 significantly increased within 2-6 h post-administration then gradually declined. MK-4 were initially low, showing slight increase, whereas rose decreased. Biochemical analyses indicated no significant sodium, chloride, potassium, albumin, or total protein levels. transient increase P observed, peaking at 12 before returning baseline. Agarose gel electrophoresis revealed distinct chylomicron bands variations VLDL HDL mobility, influenced by dietary lipids supplementation. These findings suggest effective absorption metabolism potential implications bone cardiovascular health.

Language: Английский

Identification of Marker Compounds for the Detection of Anthraquinone-Based Reactive Dyes in Foods DOI Creative Commons

L. C. Küchner,

Binh Nguyen Thanh,

Lothar Diers

et al.

Colorants, Journal Year: 2025, Volume and Issue: 4(1), P. 6 - 6

Published: Feb. 7, 2025

The detection of reactive dyes in food matrices is crucial for safety and compliance with regulations, especially since the use such products not approved. This study investigates potential using tin(II)chloride laccase to cleave anthraquinone detect their characteristic degradation as markers presence dye food. Nine blue one green were cleaved laccase. Reactions bound maize starch also carried out evaluate suitability these methods detecting matrix-bound dyes. Model matrices, including gummy candy, hard chips, spiked dyes, was analysed LC-ESI-MS/MS. Two common cleavage formed from each sample, namely 1,4-diaminoanthrahydroquinone-2-sulphonic acid (DAHS) 1-aminoanthraquinone-2-sulphonic (AAS). In all examined cases, at least could be detected. Laccase showed lower effectiveness whereas treatment acidic effective even complex matrices. These findings suggest that analysis a valuable tool

Language: Английский

Citations

0
Surfactant-based coatings for protein separation by capillary electrophoresis - A review DOI

Chirapha Prakobdi,

Laura Dhellemmes,

Laurent Leclercq

et al.

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 343945 - 343945

Published: March 1, 2025

Language: Английский

Citations

0
Advances in capillary electrophoresis for plant analysis DOI
Petr Tůma

Talanta, Journal Year: 2025, Volume and Issue: 293, P. 128171 - 128171

Published: April 17, 2025

Language: Английский

Citations

0
Rapid indirect separation of glutamine enantiomers by micellar electrokinetic chromatography. Analysis of dietary supplements DOI Creative Commons
Sandra Salido‐Fortuna,

Paloma Ruano‐Culebras,

Marı́a Luisa Marina

et al.

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(3)

Published: Feb. 1, 2024

Glutamine is the most abundant free proteinogenic α‐amino acid. It naturally produced in organism and acts as a precursor for synthesis of different biologically important molecules (such proteins or nucleotides). However, under stressful conditions, unable to produce it enough amounts function properly. Thus, glutamine (Gln)‐based supplements have become increasingly popular over last decade. Since legal regulations establish that amino acid‐based dietary must contain only L‐enantiomer not racemate, adequate chiral methodologies are required achieve their quality control. In this work, an analytical methodology based on use micellar electrokinetic chromatography proposed rapid enantiomeric determination DL‐Gln supplements. Using (+)‐1‐(9‐fluorenyl)‐ethyl chloroformate derivatizing agent ammonium perfluorooctanoate separation medium, Gln diastereoisomers formed optimal conditions were separated 8 min with resolution 2.8. The characteristics method evaluated terms linearity, precision, accuracy, limits detection/quantitation, they found appropriate analysis L‐Gln‐based

Language: Английский

Citations

1
Capillary Electrophoresis in Food Analysis DOI
María Castro‐Puyana, Samuel Bernardo‐Bermejo, Lucía Abad‐Gil

et al.

Encyclopedia of Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 27

Published: June 24, 2024

Abstract The development and application of faster more powerful analytical methodologies in Food Science is essential to face the demands from regulatory agencies, official laboratories, consumers. In this field, capillary electrophoresis (CE) nowadays a well‐established separation technique for food analysis that possesses exceptional properties related high speed efficiencies combined with small samples reagent requirements. Moreover, it very versatile since different CE modes can be employed broad range applications challenges. fact, should considered as first option when dealing highly polar, charged, or chiral analytes. past two decades, amount research work has been published, demonstrating potential analyze components variety matrices. aim article provide an overview use including summary characteristics main detectors mostly field. Besides, wide view latest (2021–2023) also provided, possibilities technique.

Language: Английский

Citations

0
Short‐term impact of vitamin K2 supplementation on biochemical parameters and lipoprotein fractions DOI
Miloš Barna, Kateřina Dunovská,

Jana Čepová

et al.

Electrophoresis, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 2, 2024

This study explored the short-term effects of vitamin K2 (VK2) supplementation on biochemical parameters (vitamin D, E, A, alkaline phosphatase, calcium, phosphorus (P), magnesium, metallothionein, triglycerides, cholesterol, high-density lipoprotein (HDL), low-density (LDL), and fractions (albumin, HDL, very (VLDL), LDL, chylomicrons). A experiment (24 h, six probands) was performed to track changes in VK2 levels after a single-dose intake (360 µg/day). Liquid chromatography-tandem mass spectrometry used monitor K (menaquinone-4 (MK-4), menaquinone-7 (MK-7), K1 [VK1]) with limit detection 1.9 pg/mL for VK1 3.8 two forms VK2. Results showed that MK-7 significantly increased within 2-6 h post-administration then gradually declined. MK-4 were initially low, showing slight increase, whereas rose decreased. Biochemical analyses indicated no significant sodium, chloride, potassium, albumin, or total protein levels. transient increase P observed, peaking at 12 before returning baseline. Agarose gel electrophoresis revealed distinct chylomicron bands variations VLDL HDL mobility, influenced by dietary lipids supplementation. These findings suggest effective absorption metabolism potential implications bone cardiovascular health.

Language: Английский

Citations

0