Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 20, 2023
Abstract
Background
Eurasian
lynx
(Lynx
Lynx
)
is
widely
distributed
in
various
habitats
Asia
and
Europe,
it
may
harbor
multiple
pathogens.
Currently,
the
information
on
protozoan
infection
scarce.
In
this
study,
we
performed
nested
polymerase
chain
reaction
(nPCR)
analysis
to
detect
intestinal
three
dead
lynxes,
northwestern
China.
Methods
Three
an
adult
female
(#1),
male
(#2),
a
cub
(#3),
were
sampled
West
Junggar
Mountain,
region
of
Xinjiang
Uyghur
Autonomous
Region.
The
intestine
samples
analyzed
using
nPCR.
We
used
primers
targeting
cytochrome
C
oxidase
subunit
I
gene
(
COI
for
detection
Sarcocystis
Eimeria
species
those
small
18S
ribosomal
RNA
rRNA
Cystoisospora
species.
nPCR-positive
products
sequenced,
aligned,
phylogenetically
analyzed.
Results
protozoa,
albifronsi
,
alpacae
felis
found
lynxes.
sample
#2
was
co-infected
with
S
.
E
addition,
only
#3.
Conclusions
To
best
our
knowledge,
detected
first
time.
lynx.
These
findings
extend
knowledge
geographical
distribution
host
range
protozoa.
One Health Bulletin,
Journal Year:
2023,
Volume and Issue:
3(1)
Published: July 1, 2023
Cryptosporidium
,
a
ubiquitous
intracellular
protozoan
parasite,
is
capable
of
causing
zoonotic
infections
in
humans
and
domestic
wild
animals.
The
primary
aim
this
review
to
summarize
the
infection
prevalence
spp.
Nepal.
Based
on
all
available
papers
published
until
November
2022,
we
have
also
summarized
pattern
diagnostic
methods,
epidemiological
factors,
prevalence,
its
genotypes
distributed
humans,
animals
environmental
samples
(mainly
water).
Among
overall
cryptosporidiosis
was
7.6%
(1328/17527),
which
highest
identified
children
(9.0%;
1225/13545),
followed
by
HIV
patients
(4.6%;
51/1107)
adults
(1.8%;
52/2875).
were
more
infected
(23.2%;
252/1088)
than
(18.2%;
63/347)
water
(32.6%;
29/89)
remained
major
source
contamination
oocysts.
Molecular
studies
regarding
transmission
Nepal
are
very
few.
However,
molecular
techniques
confirmed
(C.)
parvum
isolates
from
as
genotype
Ia2,
C.
ryanae
zebu
cattle
buffalo,
ubiquitum
swamp
deer
PCR-RFLP
sequence
analysis
SSU
rRNA
gene.
Extensive
at
level
warranted
find
out
genomic
variants
human
other
vertebrates,
both
wild,
elucidate
potential
future.
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 20, 2023
Abstract
Background
Eurasian
lynx
(Lynx
Lynx
)
is
widely
distributed
in
various
habitats
Asia
and
Europe,
it
may
harbor
multiple
pathogens.
Currently,
the
information
on
protozoan
infection
scarce.
In
this
study,
we
performed
nested
polymerase
chain
reaction
(nPCR)
analysis
to
detect
intestinal
three
dead
lynxes,
northwestern
China.
Methods
Three
an
adult
female
(#1),
male
(#2),
a
cub
(#3),
were
sampled
West
Junggar
Mountain,
region
of
Xinjiang
Uyghur
Autonomous
Region.
The
intestine
samples
analyzed
using
nPCR.
We
used
primers
targeting
cytochrome
C
oxidase
subunit
I
gene
(
COI
for
detection
Sarcocystis
Eimeria
species
those
small
18S
ribosomal
RNA
rRNA
Cystoisospora
species.
nPCR-positive
products
sequenced,
aligned,
phylogenetically
analyzed.
Results
protozoa,
albifronsi
,
alpacae
felis
found
lynxes.
sample
#2
was
co-infected
with
S
.
E
addition,
only
#3.
Conclusions
To
best
our
knowledge,
detected
first
time.
lynx.
These
findings
extend
knowledge
geographical
distribution
host
range
protozoa.
