Simultaneous Quantification of Phenolic Compounds in the Leaves and Roots of Peucedanum japonicum Thunb. Using HPLC-PDA with Various Extraction Solvents

Horticulturae, Journal Year: 2025, Volume and Issue: 11(3), P. 334 - 334

Published: March 19, 2025

This study investigated the extraction and quantification of specific phenolic compounds, including chlorogenic acid several pyranocoumarin derivatives, from leaves roots Peucedanum japonicum. Using high-performance liquid chromatography, this aimed to optimize methodologies with different solvents maximize yield bioactive compounds. The process involved meticulous steps, reflux solvent evaporation, total content was assessed using a spectrophotometric assay. results demonstrated that ethanol methanol were effective in extracting acid, yielding up 47.71 mg/g tannic equivalent MeOH extracts roots. Conversely, acetone superior for extraction, achieving coumarin exceeding 100 root extracts. Notably, pyranocoumarins found be more concentrated compared leaves, supporting hypothesis are reservoir these emphasized critical roles selections profiling compounds P. japonicum provided valuable insights future research into its pharmacological potential. findings may serve as foundation further studies, enhancing understanding medicinal properties context traditional East Asian medicine.

Language: Английский

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Multidimensional insights into squalene epoxidase drug development: in vitro mechanisms, in silico modeling, and in vivo implications

Expert Opinion on Therapeutic Targets, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Squalene epoxidase (SQLE) is a pivotal enzyme in sterol biosynthesis, catalyzing the conversion of squalene to 2,3-oxidosqualene. Beyond its core role cholesterol homeostasis, SQLE implicated cancer, hypercholesterolemia, and fungal infections, positioning it as valuable therapeutic target. We conducted comprehensive literature search across primary databases gather vitro, silico, vivo evidence on SQLE. This review explores enzyme's structural functional features, including substrate specificity catalytic mechanisms, examines inhibitor interactions. Computational methods predict - dynamics, guiding drug design, while investigations clarify SQLE's metabolic disorders tumorigenesis. Challenges include resistance study discrepancies, but emerging technologies, such cryo-electron microscopy CRISPR editing, offer new avenues for deeper exploration. an underexplored yet promising target, with particular relevance oxidative stress, ferroptosis, gut microbiota research. Overcoming current barriers through advanced technologies multidisciplinary strategies could propel SQLE-targeted treatments into clinical practice, supporting precision medicine broader translational applications.

Language: Английский

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Unravelling the epigenetic based mechanism in discovery of anticancer phytomedicine: Evidence based studies

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111743 - 111743

Published: March 1, 2025

Language: Английский

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Machine learning modeling and response surface methodology driven antioxidant and anticancer activities of chitosan nanoparticle-mediated extracts of Bacopa monnieri

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143470 - 143470

Published: April 23, 2025

Language: Английский

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