Environmental Pollution,
Journal Year:
2023,
Volume and Issue:
338, P. 122697 - 122697
Published: Oct. 5, 2023
Alkylphenolic
endocrine
disruptors
(Eds)
have
been
known
to
affect
development
of
the
descendants
multipotent
neural
crest
cells
(NCCs)
in
amphibian
embryos.
To
unravel
mechanism
head
dysgenesis
induced
by
alkylphenols
amphibians,
effect
4-octylphenol
(OP)
on
differentiation
cranial
NCCs
developing
embryos
and
tadpoles,
ex
vivo
NC
explant,
isolated
was
examined
fire-bellied
toad
Bombina
orientalis
with
0,
1,
2,
5,
10,
25
50
μM
concentrations.
Following
OP
treatment,
cartilages
were
frequently
absent
together
decreased
col2a1
mRNA
level
tadpoles.
While
lipid
hydroperoxide
(LPO),
endoplasmic
reticulum
stress
(ERS),
apoptosis,
DNA
fragmentation
significantly
increased
stage
22
neulurae
heads
45
In
neulurae,
sox9
mRNA,
master
transcription
factor
for
chondrogenic
undifferentiated
NCC
markers.
The
ectopic
found
endoderm
while
mesodermal
SOX10(+)
decreased.
from
embryos,
treatment
cellular
survival
epithelial-mesenchymal
transition
(EMT)
cell
migration.
explants,
SOX9(+)
chondrocytes
cartilage
development.
Together,
potentiated
oxidative
damage,
EMT,
migration
NCCs.
Considering
that
tissue
requires
stem
activate
molecular
at
correct
location
during
embryonic
development,
these
changes
caused
may
inhibit
sox9-dependent
NCCs,
leading
B.
Therefore,
could
be
an
important
target
OP,
provides
new
direction
estimation
risk
EDs
exposure
human
wildlife
animals.
Frontiers in Cell and Developmental Biology,
Journal Year:
2023,
Volume and Issue:
10
Published: Jan. 4, 2023
The
concept
of
dedifferentiation
and
reprogramming
mature
somatic
cells
holds
much
promise
for
the
three-front
"war"
against
tissue
damage,
cancer,
aging.
It
was
hoped
that
human
into
induced
pluripotent
state,
along
with
use
embryonic
stem
cells,
would
transform
regenerative
medicine.
However,
despite
global
efforts,
clinical
applications
remain
a
distant
dream,
due
to
associated
factors
such
as
genomic
instability,
tumorigenicity,
immunogenicity,
heterogeneity.
Meanwhile,
expression
(pluripotent)
markers
in
multiple
cancers
has
baffled
scientific
community,
it
been
suggested
dedifferentiate
"reprogram"
state
vivo
initiate
cancer.
also
aging
can
be
reversed
by
partial
vivo.
better
methods
are
needed;
using
vectors
or
Yamanaka
vivo,
example,
is
dangerous,
many
potential
anti-aging
therapies
carry
same
risks
those
described
above.
present
perspective
examines
endogenous,
very
small
embryonic-like
(VSELs).
These
naturally
tissues;
they
routinely
replace
diseased
ensure
regeneration
maintain
life-long
homeostasis,
have
ability
differentiate
adult
counterparts.
Recent
evidence
suggests
selective
expansion
epigenetically
altered
VSELs
their
blocked
differentiation.
Furthermore,
VSEL
numbers
directly
linked
lifespan
studies
long-
short-lived
transgenic
mice,
dysfunction
found
ovaries
aged
mice.
To
conclude,
greater
interest
VSELs,
address
all
three
fronts
this
war,
could
"light
at
end
tunnel."
Journal of Ovarian Research,
Journal Year:
2025,
Volume and Issue:
18(1)
Published: May 10, 2025
As
women
age,
their
ovarian
follicle
pool
naturally
declines.
However,
female
germline
stem
cells
(FGSCs)
possess
a
unique
ability
to
differentiate
into
oocytes
and
continuously
self-renew,
providing
an
effective
means
of
delaying
aging
by
replenishing
the
primordial
pool.
Therefore,
activating
FGSCs
is
critical
in
reshaping
safeguarding
function.
In
this
study,
we
investigated
biological
activity
proanthocyanidins
(PACs),
natural
antioxidants
that
exhibit
anti-aging
anti-inflammatory
properties
beneficial
for
both
male
reproduction.
Our
vivo
vitro
experiments
demonstrate
PACs
promote
proliferation
while
aging.
increase
number
follicles,
primary
corpus
luteum
reducing
cystic
elevate
estradiol
(E2)
levels
along
with
anti-mullerian
hormone
(AMH)
concentration
mice.
Additionally,
significantly
boost
time-
dose-dependently
upregulating
mRNA
&
protein
expressions
FGSCs-specific
markers
such
as
MVH
OCT4
downregulating
p53/p21
via
activation
silent
information
regulator
1(Sirt1)
signaling
pathway.
The
effects
on
FGCSs
were
found
be
impeded
Sirt1
inhibitor
EX527.
PACS
delay
premature
insufficiency
(POI)
through
regulating
Sirt1-p53-p21
pathway
involving
FGSCs.
Journal of Ovarian Research,
Journal Year:
2023,
Volume and Issue:
16(1)
Published: Feb. 1, 2023
Multiple
studies
using
single-cell
RNA
sequencing
(scRNAseq)
have
failed
to
detect
stem
cells
in
adult
ovaries.
We
maintained
that
two
populations
of
ovarian
including
pluripotent,
very
small
embryonic-like
(VSELs)
and
tissue-committed
'progenitors'
termed
(OSCs)
can
easily
be
detected
Hematoxylin
Eosin-stained
ovary
surface
epithelial
(OSE)
smears
prepared
from
both
mice
human
Most
likely
the
never
get
subjected
scRNAseq
since
they
pellet
down
only
by
centrifuging
suspension
at
1000
g
while
for
were
invariably
200-400
g.
A
recent
article
provided
further
explanation
failure
cells.
Extensive
reanalysis
data
(generated
scRNAseq)
an
advanced
software
successfully
OSCs
meiotic
markers
supporting
neo-oogenesis
But
this
remained
critical
on
biological
relevance
VSELs
their
relationship
with
OSCs.
By
carefully
studying
OSE
(which
hold
VSELs,
germ
cell
nests
GCNs),
partial
trypsin
digestion
intact
ovaries
during
different
stages
estrus
cycle,
we
delineated
novel
functions
VSELs/OSCs
vivo
under
physiological
conditions.
undergo
asymmetrical
divisions
self-renew
give
rise
slightly
bigger
which
turn
symmetrical
clonal
expansion
form
GCNs,
regular
follicle
assembly.
GCNs
been
earlier
described
fetal
culture
(from
ovaries)
response
FSH
treatment.
Dysfunction
express
ERα,
ERβ,
FSHR)
due
neonatal
exposure
endocrine
disruption
results
insufficiency
polycystic
also
implicated
cancer.
Age-related
senescence/menopause
is
dysfunction
blocked
differentiation
VSELs/OSCs.
These
findings
along
abundant
vitro
lineage
tracing
published
literature
provides
huge
scope
research,
offers
avenues
manage
pathologies
calls
re-writing
textbooks.
Stem Cell Reviews and Reports,
Journal Year:
2024,
Volume and Issue:
20(6), P. 1618 - 1635
Published: June 4, 2024
Abstract
Autoimmune
factors
play
an
important
role
in
premature
ovarian
insufficiency
(POI).
Human
amniotic
epithelial
stem
cells
(hAESCs)
have
recently
shown
promising
treatment
effects
on
chemotherapy-induced
POI.
However,
the
therapeutic
efficacy
and
underlying
mechanisms
of
hAESCs
autoimmune
POI
remain
to
be
investigated.
In
this
study,
we
showed
for
first
time
that
intravenous
transplantation
could
reside
ovary
zona
pellucida
3
peptide
(pZP3)
induced
mice
model
at
least
4
weeks.
improve
function
fertility,
alleviate
inflammation
reduce
apoptosis
granulosa
(GCs)
mice.
The
transcriptome
analysis
ovaries
vitro
co-cultivation
experiments
suggest
activation
AKT
ERK
pathways
may
key
mechanism
effect
hAESCs.
Our
work
provides
theoretical
experimental
foundation
optimizing
administration
hAESCs,
as
well
clinical
application
patients.
Graphical
Journal of Clinical Medicine,
Journal Year:
2023,
Volume and Issue:
12(16), P. 5212 - 5212
Published: Aug. 10, 2023
There
is
a
group
of
polycystic
ovary
syndrome
(PCOS)
patients
in
clinic
who
have
diminished
ovarian
reserve
(DOR)
combination.
This
study
was
designed
to
evaluate
the
differences
glucolipid
metabolism,
hypothalamic-pituitary-ovarian
(HPO)
axis-related
parameters,
and
autoimmune
antibodies
PCOS
with
without
DOR.A
total
2307
patients,
including
1757
alone
550
both
DOR,
were
enrolled
this
retrospective
study.
Parameters
HPO
measured
analyzed.The
prevalence
DOR
among
all
23.84%.
Many
such
as
follicle-stimulating
hormone
(FSH),
luteinizing
(LH),
estradiol
(E2),
prolactin
(PRL)
significantly
different
compared
DOR.
The
FSH
levels
positively
correlated
LH,
testosterone
(T),
androstenedione
(AD)
levels,
but
had
no
association
metabolism
after
adjusting
for
body
mass
index
(BMI).
Moreover,
anti-ovarian
antibody
(AOAb)
anti-21-OH
(21-OHAb)
elevated
DOR.PCOS
showed
more
chaotic
axis
antibodies,
suggesting
that
factors
may
be
cause
women
PCOS.
