Cross-disease transcriptomic analysis reveals DOK3 and PAPOLA as therapeutic targets for neuroinflammatory and tumorigenic processes DOI Creative Commons
Xingqiao Wang,

Yusong Bian,

Wei-Guang Chen

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 12, 2024

Objective Subarachnoid hemorrhage (SAH) and tumorigenesis share numerous biological complexities; nevertheless, the specific gene expression profiles underlying mechanisms remain poorly understood. This study aims to identify differentially expressed genes (DEGs) that could serve as biomarkers for diagnosis prognosis. Methods Gene datasets (GSE122063, GSE13353, GSE161870) were analyzed using machine learning algorithms logistic regression DEGs associated with both SAH tumorigenesis. Lasso receiver operating characteristic (ROC) curve analysis employed evaluate classification accuracy of these genes. Validation critical was performed through pan-cancer experimental studies, focusing on role DOK3 in modulating inflammation oxidative stress U251MG glioblastoma BV2 microglia cells. Results Fifteen common identified, PAPOLA highlighted crucial implicated neurodegenerative processes. Experimental validation demonstrated overexpression significantly reduced pro-inflammatory cytokine levels markers while enhancing antioxidant enzyme activity. Additionally, influenced tumorigenic processes such apoptosis, cell cycle regulation, proliferation, effectively mitigating LPS-induced cytotoxicity microglial Conclusions play roles related neurodegeneration, presenting themselves potential prognostic therapeutic targets. Notably, exhibits an antitumor agent anti-inflammatory antioxidative properties, offering benefits cancer neuroinflammatory conditions.

Language: Английский

Relationship Between Novel Inflammatory Indices and the Incidence of Postoperative Pneumonia After Endovascular Embolization for Aneurysmal Subarachnoid Hemorrhage DOI Creative Commons
Shaojie Li, Hongjian Li,

Weizhi Qiu

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 667 - 679

Published: Jan. 1, 2025

Accurate identification of the risk postoperative pneumonia (POP) in aneurysmal subarachnoid hemorrhage (aSAH) is essential for implementation stratified treatment. This study evaluated relevance and utility Systemic Immuno-inflammatory Index (SII) Inflammatory Response (SIRI) predicting after aSAH. Two hundred forty patients undergoing aSAH intervention were included. Differences SII SIRI between patient groups analyzed by propensity score matching (PSM). Receiver Operating Characteristic curves (ROC) used to evaluate predictive validity determine their thresholds. The association these indices with POP was assessed multivariate logistic regression restricted cubic spline (RCS), subgroup analyses performed. overall prevalence 60%, 37.5% males 62.5% females. PSM showed statistically significant differences two (P=0.032) (P=0.02). They had a high accuracy POP, AUC values 0.643 0.644, respectively. positively associated risk, independent other confounders. Moreover, further sensitivity analysis RCS supported stability this finding. Subgroup that relationship stable across subgroups. reveals potential role aSAH, provides strong basis early stratification who are at

Language: Английский

Citations

2
Exploring CX3CR1 as a prognostic biomarker and immunotherapeutic target in sarcoma DOI Creative Commons
Tengfei Li, Xun Li,

Pengcheng Kang

et al.

Translational Oncology, Journal Year: 2025, Volume and Issue: 53, P. 102283 - 102283

Published: Jan. 20, 2025

Language: Английский

Citations

0
Effects of Haptoglobin on Early Brain Injury, Vasospasm, and Lymphatic Drainage After Subarachnoid Hemorrhage in Mice DOI
Fumihiro Kawakita, Hideki Nakajima, Yume Suzuki

et al.

Stroke, Journal Year: 2024, Volume and Issue: 55(12), P. 2885 - 2895

Published: Oct. 30, 2024

BACKGROUND: Erythrolysis releases free Hb (hemoglobin), which is one of the most upstream and important molecules causing early brain injury cerebral vasospasm after subarachnoid hemorrhage (SAH). The purpose this study was to investigate if a scavenger protein Hp (haptoglobin) supplementation prevents influences lymphatic drainage mechanism in an established SAH model mice by blood injection into prechiasmatic cistern. METHODS: This consisted 4 parts, with total 317 C57BL/6 male undergoing sham or modeling, randomly followed 24-hour intracerebroventricular infusion no vehicle, 30.1 mg/mL BSA, (100%; mixture Hp1-1, Hp2-1, Hp2-2), 50% Hp, 25% 12.5% solutions from 30 minutes postmodeling. effects were evaluated at 24 48 hours RESULTS: 100%Hp decreased mortality edema until post-SAH suppressed neurological impairments, infiltrations perivascular spaces tissues, fibrinogen-positive microthrombi formation, microglial activation, caspase-dependent neuronal apoptosis, as well large-vessel on India-ink angiography compared vehicle-treated mice. up concentrations also prevented those apoptosis development hours. Immunohistochemical staining deep cervical mandibular lymph nodes demonstrated that increased sinus further administration animals. CONCLUSIONS: first showed concentrate inhibiting penetration tissues increasing

Language: Английский

Citations

0
Cross-disease transcriptomic analysis reveals DOK3 and PAPOLA as therapeutic targets for neuroinflammatory and tumorigenic processes DOI Creative Commons
Xingqiao Wang,

Yusong Bian,

Wei-Guang Chen

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 12, 2024

Objective Subarachnoid hemorrhage (SAH) and tumorigenesis share numerous biological complexities; nevertheless, the specific gene expression profiles underlying mechanisms remain poorly understood. This study aims to identify differentially expressed genes (DEGs) that could serve as biomarkers for diagnosis prognosis. Methods Gene datasets (GSE122063, GSE13353, GSE161870) were analyzed using machine learning algorithms logistic regression DEGs associated with both SAH tumorigenesis. Lasso receiver operating characteristic (ROC) curve analysis employed evaluate classification accuracy of these genes. Validation critical was performed through pan-cancer experimental studies, focusing on role DOK3 in modulating inflammation oxidative stress U251MG glioblastoma BV2 microglia cells. Results Fifteen common identified, PAPOLA highlighted crucial implicated neurodegenerative processes. Experimental validation demonstrated overexpression significantly reduced pro-inflammatory cytokine levels markers while enhancing antioxidant enzyme activity. Additionally, influenced tumorigenic processes such apoptosis, cell cycle regulation, proliferation, effectively mitigating LPS-induced cytotoxicity microglial Conclusions play roles related neurodegeneration, presenting themselves potential prognostic therapeutic targets. Notably, exhibits an antitumor agent anti-inflammatory antioxidative properties, offering benefits cancer neuroinflammatory conditions.

Language: Английский

Citations

0