ACS Applied Nano Materials,
Journal Year:
2023,
Volume and Issue:
6(20), P. 19392 - 19402
Published: Oct. 11, 2023
The
reusability
of
immobilized
lipases,
including
their
hydrolysis
activity,
is
greatly
affected
by
lipase
leakage,
mass
transfer
channel
blockage,
and
compound
deposition
on
the
outside
surface.
In
this
study,
Candida
antarctica
B
(CALB)
was
Fe3O4
nanoparticle-polyacrylonitrile
(PAN)
composite
magnetic
beads
through
phase
inversion
(L/FP).
activity
investigated
using
p-nitrophenyl
palmitate
(p-npp)
as
a
model
ester.
To
overcome
limitations
in
ester
hydrolysis,
three
modification
strategies
were
employed.
First,
covalently
nanoparticles
modified
with
methallyl
(M9)
chloro
(M13)
silane
modifiers,
which
exhibited
faster
immobilization
reduced
leaching
higher
expression
stability.
Second,
poly(ethylene
glycol)
(PEG6000)
polylactic
acid
(PLA)
added
to
precursors
physically
modify
structures
L/FP,
resulting
larger
specific
surface
area
(15.26
m2·g–1)
smaller
average
pore
size
(19
nm).
beads,
L/FP-9-M
L/FP-13-M,
demonstrated
superior
long-term
over
250
cycles.
retained
63
34%
initial
at
100th
cycle
250th
cycle,
respectively.
L/FP-13-M
55
36%
Furthermore,
even
after
repeated
uses,
unit
activities
(U·gsupport–1)
both
remained
than
Novo
435.
Current Microbiology,
Journal Year:
2023,
Volume and Issue:
80(12)
Published: Oct. 17, 2023
Abstract
Microbial
phytases
are
enzymes
that
break
down
phytic
acid,
an
anti-nutritional
compound
found
in
plant-based
foods.
These
which
derived
from
bacteria
and
fungi
have
diverse
properties
can
function
under
different
pH
temperature
conditions.
Their
ability
to
convert
acid
into
inositol
inorganic
phosphate
makes
them
valuable
food
processing.
The
application
of
microbial
the
industry
has
several
advantages.
Firstly,
adding
animal
feedstuff
improves
phosphorus
availability,
leading
improved
nutrient
utilization
growth
animals.
This
also
reduces
environmental
pollution
by
waste.
Secondly,
enhance
mineral
bioavailability
assimilation
products,
counteracting
negative
effects
on
human
health.
They
improve
taste
functional
release
bioactive
compounds
beneficial
health
effects.
To
effectively
use
industry,
factors
like
enzyme
production,
purification,
immobilization
techniques
important.
Genetic
engineering
protein
enabled
development
with
such
as
enhanced
stability,
substrate
specificity,
resistance
degradation.
review
provides
overview
phytases,
strains
produce
them,
their
industrial
applications,
focusing
new
approaches.
LWT,
Journal Year:
2025,
Volume and Issue:
219, P. 117537 - 117537
Published: Feb. 15, 2025
Plant-based
polysaccharides
(PSs)
were
used
to
immobilize
phytase
in
a
coacervate
system.
Molecular
docking
predicted
the
intermolecular
interactions
and
conformations
between
polysaccharide
correlated
them
activity
recovery
of
complex.
PSs
with
two
different
functional
groups,
sulfate
(iota
(IC),
lambda
(LC),
kappa
(KC)
carrageenan)
carboxylate
(low
methoxyl
pectin
(LMP)
sodium
alginate
(SA))
investigated.
The
optimized
conditions
for
coacervation
pH
4
phytase-to-polysaccharide
volume
ratio
12:1.
Zeta
potential
measurements,
FTIR
spectroscopy,
molecular
confirmed
that
electrostatic
hydrogen
bonding
main
driving
forces
formation.
Coacervate
complexes
formed
LMP,
SA,
or
KC
showed
high
retention
after
immobilization,
approximately
30%
yield
complex
75%
immobilization
efficiency
phytase.
lower
enzyme
observed
IC
LC
is
attributed
these
binding
enzyme's
active
site.
Overall,
this
work
contributes
body
knowledge
about
can
serve
as
guide
formulating
stable,
ingredients
plant-based
diet.
Journal of Composites Science,
Journal Year:
2023,
Volume and Issue:
7(12), P. 488 - 488
Published: Nov. 26, 2023
For
over
a
century,
enzyme
immobilisation
has
been
proven
to
be
superior
strategy
improve
catalytic
activity
and
reusability
ensure
easy
separation,
operation,
reduced
cost.
Enzyme
allows
for
an
easier
separation
of
the
from
reaction
mixture,
thus
simplifying
downstream
processing.
This
technology
protects
degradation
or
inactivation
by
harsh
conditions,
making
it
more
robust
suitable
used
in
various
applications.
Recent
strategies
methods,
such
as
adsorption,
cross-linking,
entrapment
encapsulation,
covalent
bonding,
were
critically
reviewed.
These
have
shown
promising
results
improving
stability,
activity,
A
recent
development
nanomaterials
agrowaste
renewable
carriers
is
underlined
current
review.
Furthermore,
use
gained
significant
attention
due
their
unique
properties,
high
surface
area,
mass
transfer,
biocompatibility,
sustainability.
materials
offer
outcomes
developing
efficient
sustainable
immobilised
enzymes.
state-of-the-art
better
control
reactions
enhances
reusability,
leading
cost-effective
environmentally
friendly
processes.
The
also
helps
reduce
waste
generation
promote
utilisation
resources,
further
contributing
circular
economy.
Journal of Fungi,
Journal Year:
2025,
Volume and Issue:
11(3), P. 186 - 186
Published: Feb. 26, 2025
Phytases
of
the
PhyD
class
according
to
their
pH
optimum
(7.0–7.8)
and
high
thermal
stability
can
claim
be
used
in
production
feed
supplements.
However,
today
they
have
no
practical
application
because
there
are
suitable
producers
sufficient
for
its
biotechnological
compared
PhyA
PhyC
ones.
Moreover,
most
cases,
technologies
with
enzymes
produced
secretory
form
preferable
phytases,
though
upon
microencapsulation
yeast-producing
cells,
phytase
increases
significantly
extracellular
form,
which
improves
compatibility
spray
drying
technology.
In
this
study,
we
assayed
intracellular
heterologous
expression
from
Bacillus
species
Yarrowia
lipolytica
yeast
cells.
While
technology
has
been
successfully
synthesize
Obesumbacterium
proteus,
tends
aggregate
accumulation.
Furthermore,
evaluated
prospects
encapsulated
enzymatic
activity
when
it
accumulates
cell
cytoplasm
Y.
extremophile
yeast,
a
highly
effective
platform
recombinant
proteins.
