Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 26, 2024
The
detection
of
endogenous
phenolic
compounds
(EPs)
in
food
is
great
significance
elucidating
their
bioactivity
and
health
effects.
Here,
a
novel
bifunctional
vanillic
acid-Cu
(VA-Cu)
nanozyme
with
peroxidase-like
laccase-like
activities
was
successfully
prepared.
peroxidase
mimic
behavior
VA-Cu
can
catalyze
3,3',5,5'-tetramethylbenzidine
(TMB)
to
generate
oxidized
TMB
(oxTMB).
Owing
the
high
reducing
power
EPs,
this
process
be
inhibited,
degree
inhibition
increases
increase
reaction
time.
Additionally,
owing
outstanding
laccase
VA-Cu,
it
facilitate
oxidation
various
resulting
formation
colored
quinone
imines,
catalysis
Based
on
interesting
experimental
phenomena
mentioned
above,
six-channel
sensor
array
(2
enzyme-mimic
×
3
time
points
=
6
sensing
channels)
constructed,
achieving
discriminant
analysis
nine
EPs.
In
addition,
combination
artificial
neural
network
(ANN)
algorithms
arrays
has
achieved
accurate
identification
prediction
EPs
black
tea,
honey,
grape
juice.
Finally,
portable
method
for
identifying
been
proposed
by
combining
smartphone.
ACS Applied Nano Materials,
Journal Year:
2024,
Volume and Issue:
7(9), P. 10144 - 10154
Published: April 23, 2024
Enrofloxacin
(ENR)
is
a
commonly
used
veterinary
antibiotic
and
poses
health
risk
when
present
as
residues
in
food.
This
study
describes
dual-mode
immunochromatographic
test
strip
(DITS)
biosensor
for
the
detection
of
ENR
Magnetic
graphene
oxide
nanoparticles
(MGO-NPs)
are
two-dimensional
magnetic
nanomaterials
with
large
specific
surface
area
fine
biocompatibility,
which
can
better
provide
more
adsorption
points
biological
antibodies.
Gold
(AuNPs)
stimulate
localized
plasmon
resonance
effect
under
laser
irradiation,
will
increase
temperature
to
achieve
photothermal
conversion.
The
conversion
efficiency
MGO-NPs
be
effectively
enhanced
by
combining
AuNPs
form
MGO@Au
nanocomposites
(MGO@Au-NCs).
Then,
MGO@Au-NCs
were
combined
an
monoclonal
antibody
MGO@Au@Ab
probe,
MGO@Au@Ab-DITS
was
developed.
employed
assess
samples
spanning
concentrations
from
0
1000
μg/mL.
Sample
solutions
containing
mixed
solution,
deposited
on
strip's
sample
pad,
analyzed
using
both
colorimetric
methods
employing
808
nm
infrared
imaging
instrument.
results
show
that
sensitivity
mode
sensor
0.0159°C
whereas,
standard
deviation
(SD)
limit
(LOD)
0.058
10.89
μg/mL,
respectively
quantification
(LOQ)
36.31
In
mode,
developed
5.222
au,
SD,
LOD
LOQ
40.13,
23.05
and76.85
respectively.
innovative
system
has
been
applied
detect
food
such
juice
milk,
laying
groundwork
other
drug
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 26, 2024
The
detection
of
endogenous
phenolic
compounds
(EPs)
in
food
is
great
significance
elucidating
their
bioactivity
and
health
effects.
Here,
a
novel
bifunctional
vanillic
acid-Cu
(VA-Cu)
nanozyme
with
peroxidase-like
laccase-like
activities
was
successfully
prepared.
peroxidase
mimic
behavior
VA-Cu
can
catalyze
3,3',5,5'-tetramethylbenzidine
(TMB)
to
generate
oxidized
TMB
(oxTMB).
Owing
the
high
reducing
power
EPs,
this
process
be
inhibited,
degree
inhibition
increases
increase
reaction
time.
Additionally,
owing
outstanding
laccase
VA-Cu,
it
facilitate
oxidation
various
resulting
formation
colored
quinone
imines,
catalysis
Based
on
interesting
experimental
phenomena
mentioned
above,
six-channel
sensor
array
(2
enzyme-mimic
×
3
time
points
=
6
sensing
channels)
constructed,
achieving
discriminant
analysis
nine
EPs.
In
addition,
combination
artificial
neural
network
(ANN)
algorithms
arrays
has
achieved
accurate
identification
prediction
EPs
black
tea,
honey,
grape
juice.
Finally,
portable
method
for
identifying
been
proposed
by
combining
smartphone.