Oxidative Stress in Kidney of Zebrafish due to Individual and Combined Exposure to Amoxicillin, Arsenic, and Fluoride: Involving Nrf2‐Keap1‐ARE Pathway DOI Open Access

Sunanda Mukherjee,

Shehnaz Islam, Olivia Sarkar

et al.

Journal of Applied Toxicology, Journal Year: 2025, Volume and Issue: 45(6), P. 964 - 975

Published: Feb. 5, 2025

Toxic manifestations of different antibiotics and metal compounds have been studied comprehensively in the last decades; however, their co-toxicity on aquatic organisms is poorly investigated. This study aimed to evaluate oxidative stress imposed zebrafish kidney tissue when exposed amoxicillin (AMX, 10 μg/L) alone or combination with 50 μg/L As2O3 (equivalent 37.87 As) and/or 15 mg/L NaF 6.8 F) for days. We observed increased levels cellular ROS, MDA, GSH along activity CAT enzyme all treated groups. Disrupted histoarchitecture, including degeneration tubular cells, vacuolation, necrotic spots, was indicative damage. mRNA expression responsive genes like nrf2, gpx1, hsp70, keap1, nqo1, cat, ho1 corroborated data. Translocation Nrf2 from cytoplasm nucleus its subsequent higher Moreover, mixture effects AMX + As F were more severe than other combinations, while unique exposure had minimum effects. Highlighting involvement Nrf2-Keap1-ARE pathway, these findings make us aware synergistic response AMX, As, ecosystem, putting forward a great threat humankind.

Language: Английский

Sentinel species selection for monitoring microplastic pollution: A review on one health approach DOI
Cristiana Roberta Multisanti, Carmine Merola, Monia Perugini

et al.

Ecological Indicators, Journal Year: 2022, Volume and Issue: 145, P. 109587 - 109587

Published: Oct. 22, 2022

Language: Английский

Citations

122
Get Rid of Marine Pollution: Bioremediation an Innovative, Attractive, and Successful Cleaning Strategy DOI Open Access
Valbona Aliko, Cristiana Roberta Multisanti,

Blerta Turani

et al.

Sustainability, Journal Year: 2022, Volume and Issue: 14(18), P. 11784 - 11784

Published: Sept. 19, 2022

Aquatic environmental pollution is a rather worrying and increasingly topical problem that requires the development promotion of innovative ecofriendly technologies. Pollutants in water include many common substances can reach aquatic ecosystems through several pathways including wastewater, atmosphere, ship discharges, other sources. Most these toxic compounds are internalized by organisms, leading to bioaccumulation tissues reaching any level food chain biomagnification process. These mechanisms develop into adverse effects on physiology organisms biochemical processes natural ecosystems, thus affecting animals, environments, indirectly, human health. Innovative technologies tackle marine bioremediation: suitable, biological, ecological approach enhances ability micro-organisms transform waste forms be used organisms. In this context, appear essential for detoxification systems due their metabolic activity. This review provides careful analysis characteristics main pollutants affect with focus environments. It also offers clear guidance biological strategies employed prevent, limit, remediate anthropogenic influences

Language: Английский

Citations

76
Recent Advances in Advanced Oxidation Processes for Degrading Pharmaceuticals in Wastewater—A Review DOI Open Access

Nur Nabaahah Roslan,

Harry Lik Hock Lau,

Nurul Amanina A. Suhaimi

et al.

Catalysts, Journal Year: 2024, Volume and Issue: 14(3), P. 189 - 189

Published: March 10, 2024

A large variety of pharmaceutical compounds have recently been detected in wastewater and natural water systems. This review highlighted the significance removing compounds, which are considered indispensable emerging contaminants, from Various advanced oxidation processes (AOPs), including UV-H2O2, Fenton photo-Fenton, ozone-based processes, photocatalysis, physical such as sonolysis, microwave, electron beam irradiation, regarded most viable methods to eliminate different categories discussed. All these AOPs exhibit great promising techniques, catalytic degradation process advantages, disadvantages each technique were deliberated. Heterogeneous photocatalysis employing metal oxides, particularly anatase TiO2 nanoparticles catalysts activated by UV light was reviewed terms electron–hole separation, migration charge carriers catalyst surfaces, redox potential carriers. brief overview also emphasized that TiO2-based nanomaterials photocatalysts, a combination other enhanced photocatalytic efficiency. Finally, challenges applying environmental remediation treatments degrade mass spectroscopic analysis biological activity test by-products contaminants resulting summarized.

Language: Английский

Citations

28
Assessment of the effects of non-phthalate plasticizer DEHT on the bivalve molluscs Mytilus galloprovincialis DOI Creative Commons
Miriam Porretti, Federica Impellitteri,

Alessia Caferro

et al.

Chemosphere, Journal Year: 2023, Volume and Issue: 336, P. 139273 - 139273

Published: June 19, 2023

Due to their uncontrolled use, plastics has become an environmental concern, not only for varying dimension but also the potential release of substances such as phthalates (PAEs) and non-phthalates (NPPs) into water. Phthalates are most common plasticizers non-phthalate di (2-ethylhexyl) terephthalate (DEHT) have been lately found in marine environment. Mytilus galloprovincialis is a well-known bioindicator aquatic environments due its ability accumulate wide variety xenobiotics, including plasticizers. Hence, aim this study was evaluate bioaccumulation effects NPP DEHT on M. galloprovincialis. To purpose, following exposure at 1 mg/l (DEHT1) 100 (DEHT100), accumulation tissues total lipids fatty acid (FA) composition, protein content, cell viability, recover volume changes biomarkers oxidative stress were assessed. Mussels able bioaccumulate tissues, with statistically significant increase compared control organisms. Differences FA composition observed after exposure, since C16:0, C18:0, C20:5ω-3 C22:6ω-3 significantly decreased from exposed groups. As result, SFA, MUFA PUFA affected DEHT-exposed Also, varied DEHT100-group. Considering physiological responses, both groups lost return original digestive gland (DG) cells. On other hand, gills DG by exposure. Overall, showed first time that differentially affect mussels, lipid metabolism, well cellular parameters.

Language: Английский

Citations

36
Cellular and oxidative stress responses of Mytilus galloprovincialis to chlorpromazine: implications of an antipsychotic drug exposure study DOI Creative Commons
Federica Impellitteri, Kateryna Yunko, Viktoria Martyniuk

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 13, 2023

Introduction: Bivalve molluscs like Mytilus galloprovincialis are valuable bioindicators due to their filter-feeding lifestyle, wide distribution, and ability concentrate xenobiotics. Studying the effects of pharmaceuticals on these is crucial given presence in surface waters. This study investigated response M. chlorpromazine (Cpz), an antipsychotic with antiviral activity against influenza, HIV, coronaviruses human cells. Methods: In this study, we examined 14-day impact (Cpz) model species at two concentrations (Cpz 1: 12 ng L-1 or 37 pM; Cpz 2: µg nM). To ensure controlled exposure, a stock solution was prepared introduced into tanks match intended concentrations. Seawater solutions were refreshed every 48 h. The primary focus centered evaluating cell viability, volume regulation, oxidative stress indicators. Results: Although as assessed by decreasing regulatory Regulation decrease, did not show statistically significant changes during experiment, digestive other hand, showed decrease (p < 0.01) 2 group, suggesting general health survival Biochemically, both 1 2, superoxide dismutase increased, while catalase (CAT) decreased, causing elevated lipid peroxidation thiobarbituric acid-reactive substances protein carbonyls, particularly group. level reduced glutathione (GSH) increased exposures, whereas GSSG only Consequently, GSH/GSSG ratio group only. Discussion: A comparison magnitudes anti- pro-oxidative manifestations indicated shift exposures. These findings that induces non-specific symptoms biochemical cellular disturbances even low picomolar concentration.

Language: Английский

Citations

26
Evaluation of single and combined effects of mancozeb and metalaxyl on the transcriptional and biochemical response of zebrafish (Danio rerio) DOI Creative Commons
Mahdi Banaee,

Shiva Sagvand,

Antoni Sureda

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2023, Volume and Issue: 268, P. 109597 - 109597

Published: March 6, 2023

Mancozeb and metalaxyl are fungicidal agents frequently used in combination to control fungi crops that may affect non-target organisms when entering ecosystems. This study aims evaluate the environmental effects of (MAN) Metalaxyl (MET), alone combination, on zebrafish (Danio rerio) as an experimental model. The oxidative stress biomarkers transcription genes involved detoxification were assessed after co-exposure MAN (0, 5.5, 11 μg L-1) MET 6.5, 13 mg for 21 days. Exposure induced a significant increase expression related mechanisms (Ces2, Cyp1a, Mt2). Although Mt1 gene increased fish exposed L-1 combined with MET, was down-regulated significantly other groups (p < 0.05). exposure both fungicides showed synergistic levels manifested mainly at highest concentration. 0.05) alkaline phosphatase (ALP) transaminases (AST ALT), catalase activities, total antioxidant capacity, malondialdehyde (MDA) contents hepatocytes detected, lactate dehydrogenase (LDH), gamma-glutamyl transferase (GGT) hepatic glycogen content decreased Overall, these results emphasize can synergistically (except Mt2) biochemical indicators zebrafish.

Language: Английский

Citations

25
Pharmaceutical residues in the ecosystem: Antibiotic resistance, health impacts, and removal techniques DOI
Afzal Husain Khan, Hamidi Abdul Aziz, Puganeshwary Palaniandy

et al.

Chemosphere, Journal Year: 2023, Volume and Issue: 339, P. 139647 - 139647

Published: July 27, 2023

Language: Английский

Citations

25
Cadmium induced bioaccumulation, histopathology, gene regulation in fish and its amelioration – A review DOI
Srinibas Das, Indrajit Kar, Amlan Kumar Patra

et al.

Journal of Trace Elements in Medicine and Biology, Journal Year: 2023, Volume and Issue: 79, P. 127202 - 127202

Published: May 23, 2023

Language: Английский

Citations

21
Chlorpromazine's impact on Mytilus galloprovincialis: a multi-faceted investigation DOI Creative Commons
Federica Impellitteri,

Katerina Yunko,

Giovanna Calabrese

et al.

Chemosphere, Journal Year: 2023, Volume and Issue: 350, P. 141079 - 141079

Published: Dec. 29, 2023

The antipsychotic chlorpromazine (Cpz) has raised concern as a pharmaceutical effluent due to its wide medical applications. Moreover, potent pro-oxidant properties and impact on the cell viability of marine mollusc Mytilus galloprovincialis, even at low concentrations (ng/L), have been noted. Based this evidence, in study, we investigated physiological effects Cpz M. elucidate fate within organism, terms bioaccumulation, biotransformation, byssus changes stress responses cellular thiolome. Histological indicators vitality analyses were also performed better evaluate influence drug morphology digestive gland. To end, two different (Cpz I (12 ng/L or 37 pM) II μg/L nM)) administered mussels over 14 days. accumulation gland significantly increased with water concentration (BCF II). Biochemical indicated lysosomal dysfunction, reflected elevated total Cathepsin D activity compromised membrane stability. Stress-related metal-buffering proteins (GST metallothionein) responded both concentrations. induced phase biotransformation (CYP450-dependent EROD), while triggered caspase-3 activation, indicative detoxification overload. analysis revealed atrophy, epithelial thinning, haemocyte infiltration, brown presence. Byssus showed significant alterations. In conclusion, our study underscores Cpz-induced histological posing potential implications for mussel health confirming utilisation an indication ecotoxicity.

Language: Английский

Citations

18
Effects of Single and Combined Ciprofloxacin and Lead Treatments on Zebrafish Behavior, Oxidative Stress, and Elements Content DOI Open Access
Roxana Jijie, Emanuela Paduraru,

Ira-Adeline Simionov

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(5), P. 4952 - 4952

Published: March 3, 2023

Even though the toxic effects of antibiotics and heavy metals have been extensively studied in last decades, their combined adverse impact on aquatic organisms is poorly understood. Therefore, objective this study was to assess acute a ciprofloxacin (Cipro) lead (Pb) mixture 3D swimming behavior, acetylcholinesterase (AChE) activity, lipid peroxidation level (MDA—malondialdehyde), activity some oxidative stress markers (SOD—superoxide dismutase GPx—glutathione peroxidase), essential elements content (Cu—copper, Zn—zinc, Fe—iron, Ca—calcium, Mg—magnesium, Na—sodium K—potassium) body zebrafish (Danio rerio). For purpose, were exposed environmentally relevant concentrations Cipro, Pb, for 96 h. The results revealed that exposure Pb alone with Cipro impaired exploratory behavior by decreasing elevating freezing duration. Moreover, significant deficiencies Ca, K, Mg, Na contents, as well an excess Zn level, observed fish tissues after binary mixture. Likewise, treatment inhibited AChE increased GPx MDA level. produced more damage all endpoints, while had no effect. findings highlight simultaneous presence environment can pose threat health living organisms.

Language: Английский

Citations

17