Unspecific peroxygenases: The pot of gold at the end of the oxyfunctionalization rainbow?

Current Opinion in Green and Sustainable Chemistry, Journal Year: 2023, Volume and Issue: 41, P. 100786 - 100786

Published: Feb. 16, 2023

Fungal unspecific peroxygenases (UPOs) are among the most promising biocatalysts in synthetic chemistry. The kaleidoscope of oxyfunctionalization reactions that industrial and environmental relevance offer UPOs opportunity to provide solutions many processes. However, there important shortcomings must first be overcome order convert into applied biocatalysts, which include their general poor levels heterologous functional expression, presence unwanted peroxidase activity overoxidation reactions, as well oxidative inactivation provoked by co-substrate, hydrogen peroxide. specific engineering these proteins, they participate, is being used tackle principal challenges, with a view generate portfolio highly active, selective stable variants. In this review, we will discuss some recent developments true focusing on different trends currently followed fascinating field research.

Language: Английский

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Process intensification in continuous flow biocatalysis by up and downstream processing strategies

Current Opinion in Biotechnology, Journal Year: 2022, Volume and Issue: 78, P. 102835 - 102835

Published: Nov. 2, 2022

In this review, we focus on the holistic continuous enzymatic production and put special emphasis process intensification by up- downstream processing in flow biocatalysis. After a brief introduction, provide an overview of current examples enzyme immobilization as upstream for Thereafter, unit operations strategies, namely (i) liquid-liquid extraction, (ii) adsorptive processing, (iii) crystallization precipitation. Eventually, present our perspectives future trends research field.

Language: Английский

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3D printing for flow biocatalysis

RSC Sustainability, Journal Year: 2023, Volume and Issue: 1(7), P. 1672 - 1685

Published: Jan. 1, 2023

3D printing has been elaborated in all stages of a continuous flow biocatalytic process, from the reactor to support material for biocatalyst confinement or peripheral accessories that can establish highly controlled process.

Language: Английский

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Intensified, Kilogram-Scaled, and Environment-Friendly: Chemoenzymatic Synthesis of Bio-Based Acylated Hydroxystyrenes

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(34), P. 12869 - 12878

Published: Aug. 15, 2024

Lignin-derived styrene derivatives are versatile building blocks for the manufacture of biobased polymers. As shown previously, phenol-protected hydroxystyrenes accessible under industrially sound conditions (>100 g L

Language: Английский

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Assessing the Industrial Edge of the Lipase-Mediated Oxidation of 2,5-Diformylfuran to 2,5-Furandicarboxylic Acid: Rotating Bed Reactors, an “Acyl-Donor-Free” Oxidation Concept, and Environmental Aspects

Organic Process Research & Development, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Language: Английский

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Biocatalytic conversion of fatty acids into drop-in biofuels: Towards sustainable energy sources

EFB Bioeconomy Journal, Journal Year: 2023, Volume and Issue: 3, P. 100049 - 100049

Published: March 10, 2023

Intensifying effects of global warming necessitates swift solutions for decarbonization. A big source the increasing atmospheric CO 2 is burning transportation fuels. Although electrification light vehicles an important step to reduce emissions, carbon-based fuels will still be likely power up heavy-duty transport, marine and aviation some time. Thus, fastest way decarbonize these sectors could immediate transition carbon-neutral biofuels. Recently, conversion fatty acids into hydrocarbons, as "drop-in biofuels", emerging a promising alternative fossil However, current technologies rely on energy intensive chemical methods that are not quite eco-friendly. Therefore, enzymatic hydrocarbons present benign production method. In last decade, multiple enzymes have been discovered convert free alka(e)nes. Here, we introduce with description studies performed so far, especially regarding cell-free biocatalysis.

Language: Английский

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The Role of Fungal Fuel Cells in Energy Production and the Removal of Pollutants from Wastewater

Catalysts, Journal Year: 2023, Volume and Issue: 13(4), P. 687 - 687

Published: March 31, 2023

Pure water, i.e., a sign of life, continuously circulates and is contaminated by different discharges. This emerging environmental problem has been attracting the attention scientists searching for methods treatment wastewater multiple recalcitrant compounds. Various physical chemical are used to degrade contaminants from water bodies. Traditional have certain limitations complexities bioenergy production, which motivates search new ways sustainable production treatment. Biological strategies opened avenues using oxidoreductase enzymes degradation pollutants. Fungal-based fuel cells (FFCs), with their catalysts, gained considerable among worldwide. They new, ecofriendly, alternative approach nonchemical due easy handling. FFCs efficiently in electricity power generation. article also highlights construction fungal catalytic enzymatic performance species energy wastewater.

Language: Английский

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Metal Affinity Fusion Enzyme Immobilization: Batch Process Showcase for Cascading Alcohol Oxidation and Bayer–Villiger Oxidation in Microaqueous Media

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(29), P. 10820 - 10830

Published: July 8, 2024

Language: Английский

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Improvement of the operational stability of the Novozym 435® biocatalyst in the oxidation of 2,5-furandicarboxaldehyde to 2,5-furandicarboxylic acid in a continuous packed-bed microbioreactor

Tetrahedron Letters, Journal Year: 2023, Volume and Issue: 121, P. 154475 - 154475

Published: April 6, 2023

Language: Английский

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Crosslinked Aggregates of Fusion Enzymes in Microaqueous Organic Media

ChemBioChem, Journal Year: 2023, Volume and Issue: 24(8)

Published: Feb. 7, 2023

Baeyer-Villiger monooxygenases (BVMOs) are attractive for selectively oxidizing various ketones using oxygen into valuable esters and lactones. However, the application of BVMOs is restrained by cofactor dependency enzyme instability combined with water-related downsides such as low substrate loading, capacity, water-induced side reactions. Herein, we described a redox-neutral linear cascade in-situ regeneration catalyzed fused alcohol dehydrogenase cyclohexanone monooxygenase in aqueous microaqueous organic media. The conditions have been optimized regarding concentrations well amounts enzymes cofactors Design Experiments (DoE). carrier-free immobilization technique, crosslinked aggregates (CLEAs), was applied to fusion enzymes. resultant CLEAs were proven function systems, which ratios, water contents (0.5-5 vol. %), stability systematically studied. showed promising operational (up 5 cycles) storage stability.

Language: Английский

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