A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Feb. 23, 2023

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of

Language: Английский

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NeuronBridge: an intuitive web application for neuronal morphology search across large data sets

BMC Bioinformatics, Journal Year: 2024, Volume and Issue: 25(1)

Published: March 15, 2024

Abstract Background Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. To exploit this knowledge base, researchers relate connectome’s structure neuronal function, often by studying individual neuron cell types. Vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM), enabling targeting for experimentation. However, creating line driving gene expression within single found an EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Results Here, we present NeuronBridge, web application easily rapidly putative morphological matches between We describe functionality construction NeuronBridge service, including its user-friendly graphical user interface (GUI), extensible model, serverless cloud architecture, massively parallel image search engine. Conclusions fills critical gap workflow used hundreds neuroscience around world. offer our software code, open APIs, processed integration reuse, provide service at http://neuronbridge.janelia.org .

Language: Английский

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Synaptic gradients transform object location to action

Nature, Journal Year: 2023, Volume and Issue: 613(7944), P. 534 - 542

Published: Jan. 4, 2023

Abstract To survive, animals must convert sensory information into appropriate behaviours 1,2 . Vision is a common sense for locating ethologically relevant stimuli and guiding motor responses 3–5 How circuitry converts object location in retinal coordinates to movement direction body remains largely unknown. Here we show through behaviour, physiology, anatomy connectomics Drosophila that visuomotor transformation occurs by conversion of topographic maps formed the dendrites feature-detecting visual projection neurons (VPNs) 6,7 synaptic weight gradients VPN outputs onto central brain neurons. We demonstrate how this gradient motif transforms anteroposterior looming stimulus fly’s directional escape. Specifically, discover two postsynaptic looming-responsive type promote opposite takeoff directions. Opposite these from VPNs different field regions localized threats correctly oriented escapes. For second type, graded along dorsoventral axis. generalizes across all 20 primary cell types most often arises without axon topography. Synaptic may thus be general mechanism conveying spatial features directed outputs.

Language: Английский

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Visual processing in the fly, from photoreceptors to behavior

Genetics, Journal Year: 2023, Volume and Issue: 224(2)

Published: May 2, 2023

Abstract Originally a genetic model organism, the experimental use of Drosophila melanogaster has grown to include quantitative behavioral analyses, sophisticated perturbations neuronal function, and detailed sensory physiology. A highlight these developments can be seen in context vision, where pioneering studies have uncovered fundamental generalizable principles processing. Here we begin with an overview vision-guided behaviors common methods for probing visual circuits. We then outline anatomy physiology brain regions involved processing, beginning at periphery ending descending motor control. Areas focus contrast motion detection optic lobe, circuits feature selectivity, computations support spatial navigation, contextual associative learning. Finally, look future fly neuroscience discuss promising topics further study.

Language: Английский

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Small-field visual projection neurons detect translational optic flow and support walking control

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 22, 2023

SUMMARY Animals rely on visual motion for navigating the world, and research in flies has clarified how neural circuits extract information from moving scenes. However, major pathways connecting these patterns of optic flow to behavior remain poorly understood. Using a high-throughput quantitative assay visually guided behaviors genetic neuronal silencing, we discovered region Drosophila ’s protocerebrum critical following. We used calcium imaging, optogenetics identify single cell type, LPC1, that innervates this region, detects translational flow, plays key role regulating forward walking. Moreover, population LPC1s can estimate travelling direction, such as when gaze direction diverges body heading. By linking specific types their computations behaviors, our findings establish foundation understanding nervous system uses vision guide navigation.

Language: Английский

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Descending neuron population dynamics during odor-evoked and spontaneous limb-dependent behaviors

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Oct. 25, 2022

Deciphering how the brain regulates motor circuits to control complex behaviors is an important, long-standing challenge in neuroscience. In fly, Drosophila melanogaster , this coordinated by a population of ~ 1100 descending neurons (DNs). Activating only few DNs known be sufficient drive like walking and grooming. However, what additional role larger plays during natural remains largely unknown. For example, they may modulate core behavioral commands or comprise parallel pathways that are engaged depending on sensory context. We evaluated these possibilities recording populations nearly 100 individual tethered flies while generated limb-dependent behaviors, including found largest fraction recorded encode fewer active head grooming resting. A large walk-encoding turning far weakly speed. Although odor context does not determine which behavior-encoding recruited, odors rather than behaviors. Lastly, we illustrate one can identify from DN recordings using their spatial, functional, morphological properties. These results set stage for comprehensive, population-level understanding brain’s signals regulate actions.

Language: Английский

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A visuomotor circuit for evasive flight turns in Drosophila

Current Biology, Journal Year: 2023, Volume and Issue: 33(2), P. 321 - 335.e6

Published: Jan. 1, 2023

Language: Английский

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Long-term neuropeptide modulation of female sexual drive via the TRP channel in Drosophila melanogaster

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(10)

Published: Feb. 27, 2024

Connectomics research has made it more feasible to explore how neural circuits can generate multiple outputs. Female sexual drive provides a good model for understanding reversible, long-term functional changes in motivational circuits. After emerging, female flies avoid male courtship, but they become sexually receptive over 2 d. Mating causes females reject further mating several days. Here, we report that pC1 neurons, which process courtship and regulate copulation behavior, exhibit increased CREB (cAMP response element binding protein) activity during maturation decreased after mating. This requires the neuropeptide Dh44 (Diuretic hormone 44) its receptors. A subset of neurons secretes Dh44, stimulates increases expression TRP channel Pyrexia (Pyx) neurons. This, turn, excitability drive. suppresses pyx excitability. is orthologous conserved corticotrophin-releasing family, suggesting similar roles other species.

Language: Английский

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Visual and motor signatures of locomotion dynamically shape a population code for feature detection in Drosophila

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Oct. 27, 2022

Natural vision is dynamic: as an animal moves, its visual input changes dramatically. How can the system reliably extract local features from dominated by self-generated signals? In Drosophila , diverse are represented a group of projection neurons with distinct tuning properties. Here, we describe connectome-based volumetric imaging strategy to measure visually evoked neural activity across this population. We show that jointly population, and shared gain factor improves trial-to-trial coding fidelity. A subset these neurons, tuned small objects, modulated two independent signals associated self-movement, motor-related signal, motion signal rotation animal. These inputs adjust sensitivity feature detectors locomotor cycle, selectively reducing their during saccades restoring it intersaccadic intervals. This work reveals for reliable detection locomotion.

Language: Английский

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NeuronBridge: an intuitive web application for neuronal morphology search across large data sets

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: July 21, 2022

Abstract Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. In order exploit this knowledge base, researchers target individual study function. Therefore, vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM). However, creating line for driving gene expression within single neuron found EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Here, we present NeuronBridge, web application easily rapidly putative morphological matches between datasets We describe functionality construction NeuronBridge service, including its user-friendly GUI, model, serverless cloud architecture, massively parallel image search engine. openly accessible at http://neuronbridge.janelia.org/ .

Language: Английский

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