Overexpression pattern, function, and clinical value of proteasome 26S subunit non-ATPase 6 in hepatocellular carcinoma DOI

Shengsheng Zhou,

Yuping Ye, Yi Chen

et al.

World Journal of Clinical Oncology, Journal Year: 2024, Volume and Issue: 16(2)

Published: Dec. 11, 2024

In recent years, many studies have shown that proteasome 26S subunit non-ATPase 6 (PSMD6) plays an important role in the occurrence and development of malignant tumours. Unfortunately, there are no reports on evaluation potential PSMD6 hepatocellular carcinoma (HCC). To comprehensively evaluate overexpression pattern clinical significance HCC tissues. This study integrated mRNA expression profiles from 4672 3667 non-HCC tissues, along with immunohistochemical scores 383 adjacent to assess HCC. Clustered regularly interspaced short palindromic repeats knockout technology evaluated PSMD6's essential cell growth. Functional enrichment analysis explored molecular mechanism abnormalities Drug sensitivity docking analysed effect abnormal drug cells. The results 41 external two internal datasets showed (SMD = 0.26, 95%CI: 0.09-0.42, P < 0.05) protein 2.85, 1.19-4.50, were significantly overexpressed had a significant tissues 0.40, 0.15-0.66, 0.05). inhibited growth (chronos -1). implicated ribosome biogenesis RNA splicing. Significant signalling pathways such as degradation, ribosomes, chemical carcinogenesis-reactive oxygen species. model high was associated tolerance cells drugs ML323, sepantronium bromide, GDC0810. Overexpressed effectively distinguished (AUC 0.75, 0.71-0.79). first discover is for may be involved

Language: Английский

Cholesterol effects on the tumor immune microenvironment: from fundamental concepts to mechanisms and implications DOI Creative Commons
Francisco Alejandro Lagunas‐Rangel

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 15

Published: April 9, 2025

In many cancers, the tumor microenvironment is enriched with cholesterol due to increased biosynthesis and uptake by cancer cells, resulting in accumulation of cholesterol, esters, oxysterols other metabolites various functions. These molecules serve as structural components, energy sources intracellular signaling mediators, while their toxic by-products are secreted suppress anti-tumor immune activity prevent lipid peroxidation that could induce cell apoptosis. Immune cells also contribute dynamics. Tumor-associated macrophages (TAMs) release support metabolism, myeloid-derived suppressor (MDSCs) consume essential such L-arginine, which impairs T-cell proliferation activation. Elevated dendritic migration antigen presentation and, lymphocytes, favors development a regulatory T (Treg) phenotype inhibits antitumor cytokines, further weakening response. findings suggest targeting metabolism promising strategy for treatment, improving efficacy checkpoint blockade (ICB) therapies. this manuscript, molecular mechanisms underlying effects on landscape reviewed potential cholesterol-lowering drugs enhance responses explored.

Language: Английский

Citations

0
Clinical potential and experimental validation of prognostic genes in hepatocellular carcinoma revealed by risk modeling utilizing single cell and transcriptome constructs DOI Creative Commons
Hang Deng, Xu Wang, Zhenzhen Jiang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 4, 2025

Background Hepatocellular carcinoma (HCC) is the leading cause of tumor-related mortality worldwide. There an urgent need for predictive biomarkers to guide treatment decisions. This study aimed identify robust prognostic genes HCC and establish a theoretical foundation clinical interventions. Methods The datasets were obtained from public databases then differential expression analysis used obtain significant gene profiles. Subsequently, univariate Cox regression PH assumption test performed, risk model was developed using optimal algorithm 101 combinations on TCGA-LIHC dataset pinpoint genes. Immune infiltration drug sensitivity analyses conducted assess impact these explore potential chemotherapeutic agents HCC. Additionally, single-cell employed key cellular players their interactions within tumor microenvironment. Finally, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) utilized validate roles in Results A total eight identified (MCM10, CEP55, KIF18A, ORC6, KIF23, CDC45, CDT1, PLK4). model, constructed based genes, effective predicting survival outcomes patients. CEP55 exhibited strongest positive correlation with activated CD4 T cells. top 10 drugs showed increased low-risk group. B cells as components highest interaction numbers strengths macrophages both control groups. Prognostic more highly expressed initial state cell differentiation. RT-qPCR confirmed upregulation MCM10, PLK4 tissues (p&lt; 0.05). Conclusion successfully PLK4), which provided new directions exploring pathogenesis research

Language: Английский

Citations

0
Cholesterol Targeted Catalytic Hydrogel Fueled by Tumor Debris can Enhance Microwave Ablation Therapy and Anti‐Tumor Immune Response DOI Creative Commons
Lin Shen, Zhijuan Yang, Yi Zhong

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Abstract The immunosuppressive residual tumor microenvironment (IRTM) is a key factor in the high recurrence and metastasis rates of hepatocellular carcinoma (HCC) after microwave ablation (MWA). Cholesterol‐rich fragments significantly contribute to IRTM deterioration. This study developed cholesterol‐targeted catalytic hydrogel, DA‐COD‐OD‐HCS, enhance synergy between MWA immune checkpoint inhibitors (ICIs) for HCC treatment. Cholesterol oxidase (COD), modified with dimethyl maleic anhydride (DA) release acidic IRTM, used degrade cholesterol. Oxydextran (OD) hemin‐chitosan (HCS) formed dual network gel, ensuring long‐term fixation COD hemin post‐MWA. In both vitro vivo models, DA‐COD‐OD‐HCS effectively released COD, degraded cholesterol, induced cell ferroptosis, enhancing antitumor response. Combined anti‐PD‐L1 immunotherapy, this strategy inhibited primary growth distant metastases, without side effects on adjacent tissues. work highlights that cholesterol‐targeting hydrogels fueled by debris can improve efficacy ICIs, offering novel therapeutic approach HCC.

Language: Английский

Citations

3
The protective effect of Auricularia auricula polysaccharides on cyclophosphamide-induced immunosuppression and intestinal injury: A fecal microbiota transplantation study DOI
Tianci Wang,

Zikun Jia,

Canghai An

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 62, P. 105416 - 105416

Published: Nov. 5, 2024

Language: Английский

Citations

2
Protocol to detect immune levels, abnormal metabolism, and signaling pathways in tumor tissue based on scRNA-seq obtained from patient databases DOI Creative Commons
Weiyu Bai

STAR Protocols, Journal Year: 2024, Volume and Issue: 5(2), P. 103065 - 103065

Published: May 14, 2024

Malignant tumor cells are typically more active in terms of metabolism and signal transduction compared to immune normal cells. Here, we present a protocol evaluate levels, abnormal metabolism, signaling pathways tissue based on single-cell sequencing patient data obtained from the GEO database. We describe steps for microenvironment (TIME)-based evaluation, purity assessment, identification metabolic pathways. then detail procedures screening hub genes. For complete details use execution this protocol, please refer Bai et al.1

Language: Английский

Citations

1
Protocol to develop a chemotherapy drug screening process by constructing a cancer prognostic model using public databases DOI Creative Commons
Weiyu Bai

STAR Protocols, Journal Year: 2024, Volume and Issue: 5(3), P. 103158 - 103158

Published: June 28, 2024

Drug resistance is currently the biggest challenge in cancer chemotherapy. Here, we present a protocol to develop chemotherapy drug screening process by constructing prognostic model (PM) using public databases. We describe steps for downloading code and data, preparing expression matrix metadata analysis, modeling genes, PM. then detail procedures predictive websites patients' survival based on their age, tumor stage, gene levels, risk scores. For complete details use execution of this protocol, please refer Bai et al.

Language: Английский

Citations

0
Overexpression pattern, function, and clinical value of proteasome 26S subunit non-ATPase 6 in hepatocellular carcinoma DOI

Shengsheng Zhou,

Yuping Ye, Yi Chen

et al.

World Journal of Clinical Oncology, Journal Year: 2024, Volume and Issue: 16(2)

Published: Dec. 11, 2024

In recent years, many studies have shown that proteasome 26S subunit non-ATPase 6 (PSMD6) plays an important role in the occurrence and development of malignant tumours. Unfortunately, there are no reports on evaluation potential PSMD6 hepatocellular carcinoma (HCC). To comprehensively evaluate overexpression pattern clinical significance HCC tissues. This study integrated mRNA expression profiles from 4672 3667 non-HCC tissues, along with immunohistochemical scores 383 adjacent to assess HCC. Clustered regularly interspaced short palindromic repeats knockout technology evaluated PSMD6's essential cell growth. Functional enrichment analysis explored molecular mechanism abnormalities Drug sensitivity docking analysed effect abnormal drug cells. The results 41 external two internal datasets showed (SMD = 0.26, 95%CI: 0.09-0.42, P < 0.05) protein 2.85, 1.19-4.50, were significantly overexpressed had a significant tissues 0.40, 0.15-0.66, 0.05). inhibited growth (chronos -1). implicated ribosome biogenesis RNA splicing. Significant signalling pathways such as degradation, ribosomes, chemical carcinogenesis-reactive oxygen species. model high was associated tolerance cells drugs ML323, sepantronium bromide, GDC0810. Overexpressed effectively distinguished (AUC 0.75, 0.71-0.79). first discover is for may be involved

Language: Английский

Citations

0