S-adenosyl-L-methionine alleviates the senescence of ADSCs through the PI3K/AKT/FOXO3a signaling pathway DOI Creative Commons

Lipeng Shang,

Xiaoxia Li, Xiaoyan Ding

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 28, 2023

Abstract BackgroundThe senescence of mesenchymal stem cells (MSCs) greatly compromises their therapeutic effect in regenerative medicine. Numerous studies are focusing on possible rejuvenation strategies to enhance the efficacy autologous MSC-based therapy. S-adenosyl-L-methionine (SAM) is a metabolite present all living cells. However, there little research about effects and mechanisms SAM MSCs.MethodsIn this study, adipose-derived MSC (ADSC) were assessed vitro by β-galactosidase staining, reactive oxygen species assay, cell cycle tests, Western blot. The osteogenic adipogenic differentiation ability changes also detected. Mouse models premature aging established subcutaneous injection D-Galactose (D-gal). vivo antiaging roles tested through behavioral organ coefficient, pathological morphology, expression aging-related proteins major organs, such as heart, liver, kidney. bone density distal femur, well volume, number, thickness trabecula evaluated micro-CT. molecular searched validated transcriptome sequencing, blot, immunofluorescence. Small RNA interfering was used knock down FOXO3a.ResultsIn oxidative stress–induced senescent ADSCs, ameliorated arrest, reduced activity, inhibited P53 P21, restored SIRT1, which significantly improved biological function. In addition, level ROS promoted ADSCs. Moreover, D-gal-induced mouse model aging, exercise kidney mice. Additionally, number trabeculae femur mice increased. Transcriptome sequencing results revealed that PI3K/AKT involved SAM-mediated anti-senescence MSCs. Mechanistically, activated signaling increased phosphorylation FOXO3a, resulting decrease translocation FOXO3a nucleus inhibition activity P21. Ultimately, attenuated ADSCs potential.ConclusionsIn summary, our suggested could ameliorate MSCs PI3K/AKT/FOXO3a signaling. Meanwhile, exert vivo.

Language: Английский

Astilbin attenuates apoptosis induced by cadmium through oxidative stress in carp (Cyprinus carpio L.) head kidney lymphocyte DOI
Haoran Zhang, Fuqing Zhao,

Xiaoxue Gai

et al.

Fish & Shellfish Immunology, Journal Year: 2022, Volume and Issue: 125, P. 230 - 237

Published: May 13, 2022

Language: Английский

Citations

39
Hyperbaric oxygen therapy for healthy aging: From mechanisms to therapeutics DOI Creative Commons

Qiaoyu Fu,

Ran Duan, Yu Sun

et al.

Redox Biology, Journal Year: 2022, Volume and Issue: 53, P. 102352 - 102352

Published: May 27, 2022

Hyperbaric oxygen therapy (HBOT), a technique through which 100% is provided at pressure higher than 1 atm absolute (ATA), has become well-established treatment modality for multiple conditions. The noninvasive nature, favorable safety profile, and common clinical application of HBOT make it competitive candidate several new indications, one them being aging age-related diseases. In fact, despite the conventional wisdom that excessive accelerates aging, appropriate protocols without exceeding toxicity threshold have shown great promise in therapies against aging. For thing, an extensive body basic research expanded our mechanistic understanding HBOT. Interestingly, therapeutic targets overlap considerably with those another, pre-clinical small-scale investigations validated information on efficacy from various aspects. However, generally applicable protocol to be utilized needs defined as subsequent step. It high time look back summarize recent advances concerning biological mechanisms implications promoting healthy shed light prospective directions. Here we provide first comprehensive overview field geriatric research, allows scientific community aware emerging tendency move beyond findings translational value.

Language: Английский

Citations

31
Ginseng under forest exerts stronger anti-aging effects compared to garden ginseng probably via regulating PI3K/AKT/mTOR pathway, SIRT1/NF-κB pathway and intestinal flora DOI
Mingqian Hao, Chuanbo Ding, Xiaojuan Peng

et al.

Phytomedicine, Journal Year: 2022, Volume and Issue: 105, P. 154365 - 154365

Published: July 27, 2022

Language: Английский

Citations

30
Luteolin Mitigates D-Galactose-Induced Brain Ageing in Rats: SIRT1-Mediated Neuroprotection DOI Creative Commons
Reham L. Younis,

Rehab M. El‐Gohary,

Asmaa A. Ghalwash

et al.

Neurochemical Research, Journal Year: 2024, Volume and Issue: 49(10), P. 2803 - 2820

Published: July 11, 2024

Abstract Luteolin is an essential natural polyphenol found in a variety of plants. Numerous studies have supported its protective role neurodegenerative diseases, yet the research for therapeutic utility D-galactose (D-gal)-induced brain ageing still lacking. In this study, potential neuroprotective impact luteolin against D-gal-induced was explored. Forty rats were randomly divided into four groups: control, luteolin, D-gal, and luteolin-administered D-gal groups. All groups subjected to behavioural, cholinergic function, hippocampal mitochondrial respiration assessments. Hippocampal oxidative, neuro-inflammatory, senescence apoptotic indicators detected. Gene expressions SIRT1, BDNF, RAGE assessed. histopathological studies, along with GFAP Ki67 immunoreactivity, performed. Our results demonstrated that effectively alleviated cognitive impairment reversed abnormalities. Furthermore, administration substantially mitigated hippocampus oxidative stress, dysfunction, neuro-inflammation, triggered by D-gal. Additionally, treatment considerably attenuated neuronal apoptosis upregulated SIRT1 mRNA expression. conclusion, our findings revealed D-gal-evoked senescence, improving function enhancing neuroregeneration rat model through antioxidant, senolytic, anti-inflammatory, anti-apoptotic impacts, possibly due upregulation SIRT1. could be promising modality aging-associated

Language: Английский

Citations

7
Lactobacillus plantarum Combined with Galactooligosaccharides Supplement: A Neuroprotective Regimen Against Neurodegeneration and Memory Impairment by Regulating Short-Chain Fatty Acids and the c-Jun N-Terminal Kinase Signaling Pathway in Mice DOI
Wan Wang, Cong Xu, Xuan Zhou

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2022, Volume and Issue: 70(28), P. 8619 - 8630

Published: July 11, 2022

Probiotics and prebiotics have received attention in alleviating neurodegenerative diseases. Lactobacillus plantarum (L. plantarum) 69-2 was combined with galactooligosaccharides (GOS) supplemented a d-galactose (d-gal)-induced neurodegeneration memory impairment mice model to explore its effects on the brain regulation of short-chain fatty acids. The results showed that L. plantarum-GOS supplementation inhibited d-gal-induced oxidative stress increased brain's nuclear factor erythroid 2-related 2 (Nrf2) levels. Butyrate, metabolite gut microbiota regulated by GOS, inhibits p-JNK expression, downregulates pro-apoptotic proteins expression activation inflammatory mediators, upregulates synaptic protein expression. This might be potential mechanism for GOS alleviate impairment. study sheds new light development aging-related neuroprotective dietary supplements based gut–brain axis.

Language: Английский

Citations

28
Bisphenol A aggravates renal apoptosis and necroptosis in selenium‐deficient chickens via oxidative stress and PI3K/AKT pathway DOI
Huijie Chen, Yue Zhang,

Mengmeng Zou

et al.

Journal of Cellular Physiology, Journal Year: 2022, Volume and Issue: 237(8), P. 3292 - 3304

Published: May 26, 2022

Abstract Bisphenol A (BPA) in the environment can have deleterious effects on humans and animals. BPA exert nephrotoxicity by inducing oxidative stress. Selenium (Se) deficiency specifically impair kidney tissues additionally show a synergistic effect toxicity of several environmental chemicals. However, toxic chicken whether Se produces remain poorly understood. Herein, we established exposure models model vivo vitro, described discovery path aggravation apoptosis necroptosis Se‐deficient kidneys via regulation stress phosphatidylinositol 3‐kinase/threonine kinase (PI3K/AKT) signaling pathway. We found that increased reactive oxygen species malondialdehyde levels, reduced activities catalase, GPx, superoxide dismutase, downregulated PI3K AKT expressions, activated Bcl/Bax‐Caspase 9‐Caspase 3, receptor‐interacting protein 1/mixed lineage domain‐like pathways, resulting kidney. In addition, significantly promoted expression renal BPA‐exposed kidneys. Altogether, our results showed aggravates PI3K/AKT Our findings elucidate mechanism exacerbation chickens will provide great significance for protection ecological animal health.

Language: Английский

Citations

27
Oral Administration of Deer Bone Collagen Peptide Can Enhance the Skin Hydration Ability and Antioxidant Ability of Aging Mice Induced by D-Gal, and Regulate the Synthesis and Degradation of Collagen DOI Open Access
Ke Zhang, Chenxu Zhao, Kaiyue Liu

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(11), P. 1548 - 1548

Published: May 21, 2024

Skin problems caused by aging have attracted much attention, and marine collagen peptides been proved to improve these problems, while mammalian are rarely reported. In this study, fermented deer bone peptide (FCP) non-fermented (NCP) were extracted from bone, respectively, their sequences differential proteins analyzed using LC-MS/MS technology. After they applied mice induced with D-gal, the skin hydration ability, antioxidant synthesis, degradation ability of studied. The results show that FCP NCP mainly constitute type Ⅰ collagen, segments different. vivo experiments can richness fibers in mice; skin; promote activity antioxidant-related enzymes; also through TGF-β MAPK pathways, synthesis regulated. These aging, capacity mice, regulate pathway.

Language: Английский

Citations

5
Hydrogen sulfide inhibits skeletal muscle ageing by up‐regulating autophagy through promoting deubiquitination of adenosine 5’‐monophosphate (AMP)‐activated protein kinase α1 via ubiquitin specific peptidase 5 DOI Creative Commons

Jia‐He Yang,

Jun Gao,

E Ya‐Qi

et al.

Journal of Cachexia Sarcopenia and Muscle, Journal Year: 2024, Volume and Issue: 15(5), P. 2118 - 2133

Published: Aug. 27, 2024

Abstract Background Hydrogen sulfide (H 2 S), the third gasotransmitter discovered, regulates a variety of physiological functions. Whether H S alleviates skeletal muscle ageing by regulating autophagy has not been reported. Methods Mice were administered 150 mg/kg/day D ‐galactose ( ‐gal), and C2C12 myotubes cultured in 20 g/L ‐gal to induce ageing. Sodium hydrosulfide (NaHS) was employed as an exogenous donor treatment group. The intracellular concentration quantified 7‐azido‐4‐methylcoumarin fluorescence probe. proteins involved ubiquitin‐mediated degradation AMPKα1 detected liquid chromatography tandem mass spectrometry (LC–MS/MS) co‐immunoprecipitation (Co‐IP). S‐sulfhydration USP5 tested biotin‐switch assay. Associated analysed western blot. Results NaHS found effectively restore content both gastrocnemius (+91.89%, P < 0.001) (+27.55%, 0.001). In comparison D‐gal group, observed increase mean cross‐sectional area fibres (+44.91%, 0.001), decrease collagen volume fraction (−81.32%, = reduce β‐galactosidase‐positive (−28.74%, also reverse expression atrophy F box protein (MAFbx), muscle‐specific RING finger 1 (MuRF1), Cyclin D1 p21 tissue (MAFbx: −31.73%, 0.008; MuRF1: −32.37%, 0.003; D1: +45.34%, 0.010; p21: −25.53%, 0.022) −16.38%, 0.001; −16.45%, +40.23%, −35.85%, 0.026). AMPKα1–ULK1 pathway activated up‐regulated NaHS‐treated (p‐AMPKα1: +61.61%, 0.018; AMPKα1: +30.64%, p‐ULK1/ULK1: +85.87%, 0.005; p62: −29.07%, Beclin1: +24.75%, 0.007; light chain 3 II/I [LC3 II/I]: +55.78%, 0.004) +77.49%, +26.18%, 0.022; +38.34%, 0.012; −9.02%, 0.014; +13.36%, LC3 II/I: +79.38%, 0.017; flux: +24.88%, 0.034) compared with effects on comparable those acadesine LYN‐1604, chloroquine could its LC–MS/MS Co‐IP experiments demonstrated that is deubiquitinating enzyme AMPKα1. Following knockdown USP5, activation decreased −42.10%, −43.93%, inhibited (p‐ULK1/ULK1: −27.51, +36.00, −22.15%, lost ability up‐regulate autophagy. (gastrocnemius: +62.17%, myotubes: +37.51%, 0.003) (+53.07%, 0.009) ubiquitination Conclusions promotes deubiquitination increasing thereby up‐regulating alleviating

Language: Английский

Citations

4
Lactoferrin ameliorates cognitive impairment in D-galactose-induced aging mice by regulating the PI3K/Akt/mTOR signaling pathway and the microbiome-gut-brain axis DOI
Mengqi Wang, Yi Wang, Xin Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143033 - 143033

Published: April 1, 2025

Language: Английский

Citations

0
Astilbin from Smilax china L. remarkably inhibits LPS-induced endometritis in rats via blocking positive feedback between TLR4 and IL-6R signalling pathways in a PPAR-γ-dependent manner DOI
Haixing Feng,

Chunni Li,

Jingyao Chen

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119861 - 119861

Published: April 1, 2025

Language: Английский

Citations

0