BMC Medicine,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: March 31, 2025
Pseudomonas
aeruginosa
infections
in
the
lungs
affect
millions
of
children
and
adults
worldwide.
To
our
knowledge,
no
clinically
validated
prognostic
biomarkers
for
chronic
pulmonary
P.
exist.
Therefore,
this
study
aims
to
identify
potential
markers
biofilm
lung
infections.
Here,
we
screened
expression
11
regulatory
genes
(tesG,
algD,
lasR,
lasA,
lasB,
pelB,
phzF,
rhlA,
rsmY,
rsmZ,
sagS)
associations
between
clinical
status
infection.
RNA
was
extracted
from
210
sputum
samples
patients
(n
=
70)
with
(mean
age;
29.3–56.2
years;
33
female).
Strong
formation
correlated
prolonged
hospital
stays
(212.2
days
vs.
44.4
days)
increased
mortality
(46.2%
(18)).
is
associated
tesG
(P
0.001),
influencing
extended
intensive
care
unit
0.002)
or
hospitalisation
pneumonia
risk
0.006),
0.001).
Notably,
linked
modulation
systemic
inflammatory
responses
predicts
biomass.
This
provides
first
dataset
levels
as
a
predictive
biomarker
Infection and Drug Resistance,
Journal Year:
2024,
Volume and Issue:
Volume 17, P. 449 - 462
Published: Feb. 1, 2024
Abstract:
Klebsiella
pneumoniae
(
K.
),
a
significant
contributor
to
the
global
challenge
of
antibiotic
resistance,
is
not
only
ubiquitous
component
human
microbiome
but
also
potent
pathogen
capable
causing
spectrum
diseases.
This
review
provides
thorough
analysis
intricate
interactions
between
and
immune
system,
elucidating
its
substantial
impact
on
metabolic
processes.
We
explore
mechanisms
employed
by
evade
manipulate
responses,
including
molecular
mimicry,
modulation,
biofilm
formation.
The
further
investigates
bacterium
'
s
influence
pathways,
particularly
glycolysis,
highlighting
how
these
exacerbate
disease
severity.
emergence
multidrug-resistant
extremely
drug-resistant
strains
within
Enterobacteriaceae
family
has
heightened
public
health
crisis,
underscoring
urgency
for
comprehensive
research.
investigate
roles
host's
complement
autophagy,
cell
death
mechanisms,
various
cytokines
in
combating
infections,
shedding
light
areas
that
warrant
academic
investigation.
Additionally,
discusses
challenges
posed
K1-
K2-capsule
polysaccharides
vaccine
development
due
their
complex
structures
adhesive
properties.
Acknowledging
limited
availability
effective
antimicrobials,
this
advocates
exploring
alternative
approaches
such
as
immunotherapeutics,
vaccinations,
phage
therapy.
consolidate
current
knowledge
,
covering
classical
non-classical
subtypes,
antimicrobial
resistance-mediated
genes,
virulence
factors,
epidemiological
trends
isolation
resistance
rates.
advances
our
understanding
underscores
imperative
ongoing
research
collaborative
efforts
develop
new
prevention
treatment
strategies
against
formidable
pathogen.
Keywords:
pathogenic
mechanism,
drug-resistant,
strategy
Biomedicine & Pharmacotherapy,
Journal Year:
2023,
Volume and Issue:
168, P. 115793 - 115793
Published: Oct. 29, 2023
Given
the
rise
of
multidrug-resistant
(MDR)
Pseudomonas
aeruginosa
infections,
alternative
treatments
are
needed.
Anti-pseudomonal
phage
therapy
shows
promise,
but
its
clinical
application
is
limited
due
to
development
resistance
and
a
lack
biofilm
penetration.
Recently,
adjuvants
like
CaEDTA
have
shown
ability
enhance
effectiveness
combined
antimicrobial
agents.
Here,
we
tested
phage-adjuvant
combination
demonstrated
intranasally
inhaled
(KKP10)
+
in
addition
ceftazidime/avibactam
(CZA)
for
chronic
P.
lung
infections.
The
results
emphasize
that
intranasal
inhalation
along
with
can
successfully
re-sensitize
MDR
CZA
triple
treatment.
This
promising
approach
potential
as
respiratory
tract
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Oct. 26, 2024
Pasteurella
aerogenes
has
been
implicated
in
reproductive
disorders
sows,
yet
its
prevalence
and
characteristics
vaginal
discharge
are
not
well
understood.
This
study
aimed
to
detect
P.
sow
samples
investigate
antibiotic
resistance
profile,
toxin
genes,
toxicity.
was
isolated
from
40%
(8/20)
of
samples.
Antimicrobial
susceptibility
testing
revealed
universal
amoxicillin-clavulanate
(4:1),
with
87.5%
isolates
also
resistant
oxytetracycline,
amoxicillin,
ceftriaxone,
enrofloxacin.
The
colistin
gene
mcr-2
detected
75%
isolates,
while
class
1
integron
(int1)
found
12.5%.
pax
cluster
present
isolates.
Toxicity
assays
using
Panagrellus
redivivus
demonstrated
dose-dependent
effects
supernatant
containing
toxins.
represents
the
first
report
isolation
Thailand.
high
resistance,
presence
gene,
toxicity
toxin-positive
suggest
that
may
be
an
underestimated
factor
swine
health.
These
findings
highlight
need
for
further
investigation
into
role
potential
impact
on
production.
Journal of Current Science and Technology,
Journal Year:
2024,
Volume and Issue:
14(2)
Published: May 2, 2024
The
rise
in
drug
resistance
poses
escalating
challenges
for
antibacterial
medications,
leading
to
an
urgent
demand
the
exploration
and
innovation
of
new
drugs.
Fagraea
fragrans
Roxb.,
belonging
Gentianaceae
family,
is
one
common
herbal
medicines
which
can
be
found
abundantly
Southeast
Asia.
secoiridoid
glucoside
swertiamarin,
major
compounds
F.
leaf,
exhibits
antimicrobial
effects.
To
guarantee
medicinal
effectiveness
leaves,
it
essential
identify
a
standardized
analytical
method
quantifying
active
compound.
In
this
study,
optimized
HPLC
following
ICH
guideline
was
validated
quantitative
analysis
swertiamarin
content
leaf
terms
linearity
(y
=
5733.5x
-
369.1;
R2
0.9999),
accuracy
(93.57-96.39%
recovery),
precision
(0.91%
RSD
repeatability
precision;
1.19%
intermediate
precision),
limit
detection
(0.73
µg/mL),
quantitation
(2.23
specificity
(peak
purity
index
0.999995)
robustness
(%
<1).The
maximum
wavelength
at
238
nm.
amount
extract
conducted
from
0.0259
±
0.0005
g/100
g
crude
drug.
exhibited
activity
against
clinical
isolates
Enterobacter
cloacae,
Klebsiella
pneumonia,
Escherichia
coli
0.125
mg/mL,
Pseudomonas
aeruginosa
0.5,
showing
minimum
inhibitory
concentration
(MIC)
values.
Whereas
even
lower
MIC
developed
effectively
determines
as
chemical
marker
ensure
potential
leaves.
Trends in Sciences,
Journal Year:
2024,
Volume and Issue:
21
Published: July 24, 2024
The
growing
prevalence
of
multidrug-resistant
Enterococcus
faecalis
infection
has
become
a
global
concern.
There
is
demand
for
alternative
antibacterial
agents,
such
as
herbal
alternatives,
Cannabidiol,
that
are
cost-effective,
non-toxic
and
efficient.
This
study
investigates
the
Minimum
Inhibitory
Concentration
(MIC)
planktonic
cells
Biofilm
Eradication
(MBEC)
biofilm
formation
in
multidrug-
resistance
isolates
E.
isolates,
particularly
focusing
cannabidiol
activity.
with
strong
presence,
vancomycin,
levofloxacin,
daptomycin
displayed
high
MIC
MBEC
values.
Cannabidiol
exhibited
significantly
lower
(1
μg/mL)
low
(2
μg/mL).
Moreover,
at
concentrations
µg/mL),
demonstrated
notable
reductions
biovolume,
bacterial
cell
viability
colony-forming
unit
compared
to
daptomycin.
mice
treated
(100
mg/kg)
significant
reduction
load
internal
organs
increased
survival.
In
conclusion,
findings
underscore
promising
antibiofilm
properties
against
faecalis,
indicating
its
potential
novel
therapeutic
agent.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Oct. 9, 2024
Abstract
Klebsiella
pneumoniae
has
been
identified
as
one
of
the
most
important
opportunistic
pathogens
responsible
for
nosocomial
infections.
Antibiotic
resistance
and
ability
to
form
biofilms
are
two
main
factors
involved
in
persistence
Conventional
detection
methods
involve
culture
isolation
identification
followed
by
biofilm
assay
that
takes
48–72
h.
Timely
biofilm-forming
resistant
is
essential
appropriately
treat
infection
with
right
dose
combinations.
The
present
study
focuses
on
evaluating
an
RT-PCR
panel
using
mrk
D,
pga
C,
wca
J
genes
screen
K.
from
ETA/BAL
specimens.
accurately
harboring
samples
a
limit
1
ng/µl
total
RNA.
Representative
culture-negative-PCR-positive
were
subjected
metagenomics
which
reads
these
confirming
specificity
RT-PCR.
D
C
act
specific
whereas
acts
negative
marker
.
In
addition,
results
correlated
well
phenotypic
assay.
This
first
its
kind
rapid
result
this
highlights
based
coupled
clinical
conditions
would
be
appropriate
emerging
infections
or
prevent
re-infections
settings.
Infection and Drug Resistance,
Journal Year:
2024,
Volume and Issue:
Volume 17, P. 1281 - 1289
Published: March 1, 2024
Since
the
introduction
of
ceftazidime-avibactam
(CZA)
in
Chinese
market,
accumulating
clinical
evidence
has
substantiated
its
efficacy
treatment
infections
caused
by
carbapenem-resistant
gram-negative
bacteria
(CR-GNB).
Nevertheless,
an
ongoing
debate
persists
concerning
choice
between
monotherapy
and
combination
therapy
when
devising
anti-infection
protocols.
BMC Genomics,
Journal Year:
2024,
Volume and Issue:
25(1)
Published: April 17, 2024
Abstract
Klebsiella
pneumoniae
is
a
Gram-negative
Enterobacteriaceae
that
classified
by
the
World
Health
Organisation
(WHO)
as
Priority
One
ESKAPE
pathogen.
South
and
Southeast
Asian
countries
are
regions
where
both
healthcare
associated
infections
(HAI)
community
acquired
(CAI)
due
to
extended-spectrum
β-lactamase
(ESBL)-producing
carbapenem-resistant
K.
(CRKp)
of
concern.
As
can
also
exist
harmless
commensal,
spread
resistance
genotypes
requires
epidemiological
vigilance.
However
there
has
been
no
significant
study
carriage
isolates
from
healthy
individuals,
particularly
in
Asia,
specially
Malaysia.
Here
we
describe
genomic
analysis
respiratory
obtained
Orang
Ulu
Asli
communities
Malaysian
Borneo
Peninsular
Malaysia
respectively.
The
majority
were
species
complex
(KpSC)
1
(
n
=
53,
89.8%).
Four
variicola
subsp.
(KpSC3)
two
quasipneumoniae
similipneumoniae
(KpSC4)
found.
It
was
discovered
30.2%
16)
KpSC1
ST23,
11.3%
6)
ST65,
7.5%
4)
ST13,
13.2%
7)
ST86.
Only
eight
encoded
ESBL,
but
importantly
not
carbapenemase.
Thirteen
carried
yersiniabactin,
colibactin
aerobactin,
all
which
harboured
rmpADC
locus
therefore
characterised
hypervirulent.
Co-carriage
multiple
strains
minimal.
In
conclusion,
most
KpSC1,
one
common
sequence
types
previously
found
cases
infection.
A
proportion
hypervirulent
(hv
Kp
)
however
antibiotic
low.
Infection and Drug Resistance,
Journal Year:
2023,
Volume and Issue:
Volume 16, P. 7227 - 7237
Published: Nov. 1, 2023
Ceftazidime-avibactam
(CAZ-AVI)
is
a
new
cephalosporin/β-lactamase
inhibitor
combination
that
received
clinical
approval
in
China
2019.
This
study
aims
to
investigate
the
efficacy
and
safety
of
CAZ-AVI
treatment
Klebsiella
pneumoniae
(KP)
infection
hospital,
differences
among
various
sites
between
monotherapy
therapy,
providing
valuable
insights
for
its
further
application.Patients
who
used
January
2019
April
2023
were
identified
through
hospital
information
system.
Demographic
information,
details
site,
KP
strain's
drug
sensitivity
report,
duration,
therapies,
adverse
reactions
(ADR),
28-day
survival
recorded.
Clinical
microbiological
efficacies
analyzed
using
SPSS
23.0
software
compare
different
therapies.The
overall
effective
response
(CR)
rate
against
was
62.13%,
with
favorable
microbial
(MR)
65.68%
63.91%.
No
significant
difference
occurred
CR
(P
=
0.709
0.862,
respectively).
The
MR
abdominal
infections
slightly
lower
than
other
0.021).
CR,
MR,
therapy
present
values
0.649,
0.123,
0.280,
incidence
ADR
1.78%,
including
increased
creatinine,
elevated
transaminase,
hematuria,
thrombocytopenia.CAZ-AVI
demonstrates
good
infections.
similar
across
sites,
did
not
show
an
advantage
over
monotherapy.
Further
studies
are
warranted.
It
should
be
noted
may
induce
thrombocytopenia
hematuria.
