Dry ginger and Schisandra chinensis modulate intestinal flora and bile acid metabolism to treatment asthma

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 27, 2025

Background and aims Zingiber officinale Rosc (Dry ginger) Schisandra chinensis (Turcz.) Baill (Schisandra chinensis) drug pairs (DSP) are often used as for the treatment of asthma, these two traditional Chinese medicines (TCM) also core components multiple TCM. However, its specific pharmacological mechanism needs further research. The aim this study is to investigate effects DSP on intestinal flora bile acid metabolism in rats with asthma caused by cold, provide experimental evidence clinical application. Materials methods Sixty male divided into five groups, 12 per group. Except control other groups use method “abdominal injection “OVA + ice water swimming” establish cold cough models. After administration cycle over, an optical microscope count detect eosinophils (EOS) neutrophils (Neu) bronchoalveolar lavage fluid (BALF); Enzyme-Linked Immunosorbent Assay (ELISA) detects Immunoglobulin E (IgE), Interleukin-13 (IL-13), Interleukin-4 (IL-4), Tumor Necrosis Factor-α (TNF-α), Interferon-γ (INF-γ); Western Blot (WB) Reverse Transcription-Polymerase Chain Reaction (RT-PCR) analysis was Forkhead box protein P3 (Foxp3) Transforming Growth Factor-beta 1 (TGF-β1) proteins expression. In addition, Using 16S rDNA sequencing reveal role effect gut microbiome after treatment. We examined Farnesoid X Receptor (FXR) expression, finally ultra performance UPLC-MS/MS analyze acids (BAs) contentin rats. Results inhibits inflammation. It alleviates inflammatory factors, suppresses response asthmatic regulating FOXP3 TGF-β1 Treg cells, reduces tissue damage. treatment, changed: harmful bacteria like Streptococcus decreased, while beneficial such Candidatus - Arthromitus Ligilactobacillus increased. These changes can be potential markers DSP-intervened asthma. Also, increased FXR expression changed spectrum: Deoxycholic (DCA) increased, allocholic (ACA), glycolithocholic (GLCA), glycochenodeoxycholic (GCDCA) andglycoursodeoxycholic (GUDCA) decreased. Conclusion This has preliminarily revealed that alleviating inflammation levels, regulate proteins, impact microbiota metabolism, thereby exerting improving providing a reference application medicine

Language: Английский

A Network Analysis Framework for Traditional Chinese Medicine in Treating Alzheimer’s Disease: From Core Herbal Pairs to Key Component Networks

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION), Journal Year: 2025, Volume and Issue: unknown, P. 100560 - 100560

Published: May 1, 2025

Language: Английский

Kemin capsule ameliorates post-infectious cough by modulating the PI3K/AKT signaling pathway and TRPA1/TRPV1 channels

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 118837 - 118837

Published: Sept. 1, 2024

Language: Английский