Anticipation and Verification of Dendrobium-Derived Nanovesicles for Skin Wound Healing Targets, Predicated Upon Immune Infiltration and Senescence

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 1629 - 1644

Published: Feb. 1, 2024

Background: Dendrobium , with profound botanical importance, reveals a rich composition of bioactive compounds, including polysaccharides, flavonoids, alkaloids, and diverse amino acids, holding promise for skin regeneration. However, the precise mechanism remains elusive. Seeking potent natural remedy wound healing, exocyst vesicles were successfully isolated from . Aims Study: This investigation aimed to employ bioinformatics in vivo experiments elucidate target genes -derived nanovesicles focusing on immune infiltration senescence characteristics. Materials Methods: C57 mice experienced facilitated healing through (DDNVs). Bioinformatics analysis GEO database mining identified crucial by intersecting immune-related, senescence-related, PANoptosis-associated genes. The underwent validation. Results: DDNVs remarkably accelerated mice. revealed abnormal expression patterns pan-apoptosis-related genes, highlighting an overexpressed IL-1β downregulated IL-18 model group, Exploration signaling pathways included IL-17, NF-kappa B, NOD-like receptor, Toll-like receptor pathways. In confirmed DDNVs' efficacy suppressing expression, enhancing healing. Conclusion: Plant-derived (PDNV) emerged as natural, reliable, productive approach uptake mouse tissues, labeled fluorescent dye, led enhanced Notably, overexpression cells played key role. intervention effectively suppressed accelerating tissue repair. Keywords: plant-derived nanovesicles, factors, cellular aging, injury recovery

Language: Английский

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New perspective on the immunomodulatory activity of ginsenosides: Focus on effective therapies for post-COVID-19

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 165, P. 115154 - 115154

Published: July 14, 2023

More than 700 million confirmed cases of Coronavirus Disease-2019 (COVID-19) have been reported globally, and 10–60% patients are expected to exhibit "post-COVID-19 symptoms," which will continue affect human life health. In the absence safer, more specific drugs, current multiple immunotherapies failed achieve satisfactory efficacy. Ginseng, a traditional Chinese medicine, is often used as an immunomodulator has in COVID-19 treatment tonic increase blood oxygen saturation. Ginsenosides main active components ginseng. this review, we summarize ways ginsenosides post-COVID-19 symptoms, including inhibition lipopolysaccharide, tumor necrosis factor signaling, modulation chemokine receptors inflammasome activation, induction macrophage polarization, effects on Toll-like receptors, nuclear kappa-B, mitogen-activated protein kinase pathway, lymphocytes, intestinal flora, epigenetic regulation. virus-mediated tissue damage, local or systemic inflammation, immune modulation, other links, thus alleviating respiratory pulmonary reducing cardiac burden, protecting nervous system, providing new ideas for rehabilitation with symptoms. Furthermore, analyzed its role strengthening body resistance eliminate pathogenic factors from perspective ginseng-epidemic disease highlighted challenges clinical applications. However, benefit modulating organismal imbalance needs be further evaluated better validate pharmacological mechanisms associated their efficacy determine individualized therapy.

Language: Английский

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The beneficial potential of ginseng for menopause

Journal of Ginseng Research, Journal Year: 2024, Volume and Issue: 48(5), P. 449 - 453

Published: May 31, 2024

Ginseng has a wide range of pharmacological activities including immune, endocrine, cardiovascular, and central nervous system effects. Korean red ginseng (KRG) is produced by repetitions steaming drying to extend preservation. During this process, the components undergoes changes. Especially, ginsenoside-Rg3 unique component in KRG. KRG long been used not only as food supplement but also treatment for various diseases originally South Korea which later spread China Japan. Pandemic Coronavirus disease 2019 (COVID-19) have affected men women differentially were more vulnerable COVID-related distress. COVID-19 could aggravate menopause-related disturbances complementary alternative medicinal plants aided middle-aged several symptoms during after pandemic. This review aimed beneficial effects on menopausal gynecological cancer. contains compounds, possess anti-inflammatory, anti-cancer, antioxidant immune-modulating properties. aids effective improving andropause.

Language: Английский

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Roles of increased NUCKS1 expression in endometriosis

BMC Women s Health, Journal Year: 2023, Volume and Issue: 23(1)

Published: Aug. 15, 2023

Abstract Background Endometriosis is still a difficult problem for women. The Nuclear Ubiquitous Casein and cyclin-dependent Kinase Substrate 1 (NUCKS1) gene located on human chromosome 1q32.1. It encodes the NUCKS1 protein, 27 kDa nuclear DNA binding protein that plays an important role in cell growth proliferation. development of many diseases. However, its endometriosis unclear. Methods Ectopic endometrial tissues normal tissue specimens were collected, expression NUCKS1, NF-κB PI3K was detected by RT-qPCR immunohistochemistry. Inhibition hEM15A cells, study changes viability, apoptosis, migration CCK8 assay, flow cytometry, wound-healing western blot ELISA techniques. comparison differences between two groups implemented using unpaired sample t test or Mann-whitney U test. One-way analysis variance Kruskal-wallis used comparisons among three groups. Results (1) highly expressed tissues. (2) decreases viability capability migration, increases apoptosis cells. (3) Expressions are increased tissues, inhibition levels (4) VEGF. Conclusion overexpressed endometriosis, inhibits apoptosis. promotes progress through activating pathways, VEFG also involved this process.

Language: Английский

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Traditional Chinese medicine (TCM) and nutraceuticals for the prophylaxis and treatment of COVID-19 variants: A review

Pharmacological Research - Modern Chinese Medicine, Journal Year: 2023, Volume and Issue: 9, P. 100330 - 100330

Published: Nov. 25, 2023

The COVID-19 pandemic has led to increased interest in herbal supplements and nutraceuticals as alternative treatments. This review looks at the evidence on their use prevent treat variants. It talks about possible pros cons stresses need for more research this area fully understand how well they work against virus. In order conduct review, a comprehensive search of relevant literature was performed. Studies examining efficacy preventing treating variants were included. data from these studies analyzed determine potential benefits limitations Additionally, also considered any side effects or interactions with other medications. found that while some have variants, effectiveness is uncertain. Vitamin C zinc may boost immune system, but combating virus explores TCM COVIR-19 Some interact certain medications, highlighting consultation healthcare professionals. Further needed safety study traditional Chinese medicine. sparked debate treatments, suggesting can system combat virus, others call research. Safety concerns between medications arise. A understanding treatments crucial optimal care during pandemic. Healthcare professionals should stay updated evidence, allowing informed discussions patients risks. balanced approach considering individual patient needs essential. play role guiding making decisions supplements, ensuring are not used substitutes. They help risks adopting considers both holistic essential current situation promotes overall well-being.

Language: Английский

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NUCKS1 is Overexpressed in Endometriosis

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 9, 2023

Abstract Background Endometriosis is still a difficult problem for women.The Nuclear Ubiquitous Casein and cyclin-dependent Kinase Substrate 1(NUCKS1)gene located on human chromosome 1q32.1. It encodes the NUCKS1 protein, 27 kDa nuclear DNA binding protein that plays an important role in cell growth proliferation. development of many diseases. However, its endometriosis unclear. Methods Ectopic endometrial tissues normal tissue specimens were collected, expression NUCKS1, NF-κB PI3K was detected by RT-qPCR immunohistochemistry. Inhibition hEM15A cells, study changes viability, apoptosis, migration CCK8 assay, flow cytometry, wound-healing western blot ELISA techniques. The comparison differences between two groups implemented using unpaired sample t test or Wilcoxon signed rank sum test. One-way analysis variance Kruskal-wallis used comparisons among three groups. Results (1) highly expressed tissues. (2) decreases viability capability migration, increases apoptosis cells. (3) Expressions are increased tissues, inhibition levels (4) VEGF. Conclusion overexpressed endometriosis, inhibits apoptosis. promotes progress through activating pathways, VEFG also involved this process.

Language: Английский

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