Enzymatic Synthesis of Phytosterol Esters Using Recombinant E. Coli Whole Cell Expressing Lipase from Burkholderia Sp

Published: Jan. 1, 2025

Language: Английский

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Lipase Production Through Solid-State Fermentation: Partial Purification and Application for Omega-6 Enrichment in Fish Oil

Waste and Biomass Valorization, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 25, 2025

Language: Английский

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The current research status of immobilized lipase performance and its potential for application in food are developing toward green and healthy direction: A review

Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(2)

Published: Feb. 1, 2025

Abstract Immobilized lipases have received great attention in food, environment, medicine, and other fields due to their easy separation, high stability (temperature, pH), storage properties. After immobilization, lipase transforms from a homogeneous heterogeneous state, making it easier recover the reaction substrate achieve recycling, which is line with concept of green chemistry reduces protein contamination product. There are various materials for enzyme including polysaccharides natural sources, inorganic compounds, carbon nanotubes, metal–organic framework materials, so forth. Magnetic immobilization carriers been widely studied ability separation by adding magnetic field. Its method can be simply divided into two categories: physical action (adsorption, embedding) chemical binding (covalent, cross‐linking). Some studies mainly discuss support methods, applications immobilized food. On this basis, our review also focuses on changes crosslinking agents lipases, different methods promote new trends study proposes prospects research food industry.

Language: Английский

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Optimization of the Full Hydrolysis of Babassu Oil by Combi-Lipases

Catalysts, Journal Year: 2025, Volume and Issue: 15(3), P. 209 - 209

Published: Feb. 22, 2025

The concept of combi-lipases is herein explored in the full hydrolysis babassu oil. commercially immobilized lipases from Candida antarctica (form B) (Novozym® 435), Rhizomucor miehei (Lipozyme® RM-IM), and Thermomyces lanuginosus TL-IM) were evaluated as single combined biocatalysts by a mixture design with triangular surface. As result, after evaluating response desirability profiling for all biocatalysts, best biocatalyst reaction was composed 75% Lipozyme® RM-IM, 17% Novozym® 435, 8% TL-IM, reaching (>99%) 4 h reaction. Subsequently, such employed optimization shorter time (3 h). After Taguchi method, reached under optimized conditions (9 wt.% content, 1:2 (oil/water), 40 °C, 180 rpm). Under conditions, maintained 70% their initial activity 10 cycles. antimicrobial against some most common environmental bacteria obtained free fatty acids (FFAs) also evaluated. FFAs inhibited more than 90% growth S. aureus, E. coli, P. aeruginosus when using mg FFAs/mL.

Language: Английский

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Highly efficient enzymatic enrichment of n-3 polyunsaturated fatty acid glycerides via interfacial biocatalysis in Pickering emulsions

Food Chemistry, Journal Year: 2024, Volume and Issue: 470, P. 142683 - 142683

Published: Dec. 28, 2024

Language: Английский

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