Crosslinker-free in situ hydrogel induces self-aggregation of human dental pulp stem cells with enhanced antibacterial activity

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101451 - 101451

Published: Jan. 5, 2025

Recently, injectable hydrogels have garnered significant attention in tissue engineering due to their controlled flowability, strong plasticity, adaptability, and good biocompatibility. However, research on readily situ-forming capable of forming functional three-dimensional (3D) condensations remains limited. This study explores the development evaluation a carboxymethyl chitosan (CMCTS)/oxidized hyaluronic acid (oHA) hydrogel incorporated with silver sulfadiazine (AgSD) for applications inherent antibacterial activity. Through physicochemical analysis, optimal formulation CMCTS/oHA was established. The demonstrated excellent injectability, enabling minimally invasive situ delivery. In vitro cytotoxicity assays identified 0.1 % AgSD as concentration, supporting cell proliferation while exhibiting antimicrobial efficacy against S. mutans E. faecalis. vivo studies revealed complete degradation biocompatibility, no adverse reactions. hydrogel's ability form 3D aggregates support regeneration underscores its potential future applications. Consequently, CMCTS/oHA/AgSD developed this holds application wide range bioengineering fields, including substance delivery systems engineering, indicating clinical application.

Language: Английский

Crosslinker-freein situhydrogel induces self-aggregation of human dental pulp stem cells with enhanced antibacterial activity

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

ABSTRACT Recently, injectable hydrogels have garnered significant attention in tissue engineering due to their controlled flowability, strong plasticity, adaptability, and good biocompatibility. However, research on readily situ -forming capable of forming functional three-dimensional (3D) condensations remains limited. This study explores the development evaluation a carboxymethyl chitosan (CMCTS) / oxidized hyaluronic acid (oHA) hydrogel incorporated with silver sulfadiazine (AgSD) for applications inherent antibacterial activity. Through physicochemical analysis, optimal formulation CMCTS/oHA was established. The demonstrated excellent injectability, enabling minimally invasive delivery. In vitro cytotoxicity assays identified 0.1% AgSD as concentration, supporting cell proliferation while exhibiting antimicrobial efficacy against S. mutans E. faecalis . vivo studies revealed complete degradation biocompatibility, no adverse reactions. hydrogel’s ability form 3D aggregates support regeneration underscores its potential future applications. Consequently, CMCTS/oHA/AgSD developed this holds application wide range bioengineering fields, including substance delivery systems engineering, indicating clinical application.

Language: Английский