Ultrastructure of the female reproductive organs of the diving beetle Deronectes moestus incospectus (Leprieur, 1876) (Dytiscidae, Hydroporinae) DOI Creative Commons
Romano Dallai, David Mercati, Paulo Henrique Rezende

et al.

Arthropod Structure & Development, Journal Year: 2023, Volume and Issue: 75, P. 101287 - 101287

Published: July 1, 2023

We describe the ultrastructure of female reproductive organs Deronectes moestus (Dytiscidae Hydroporinae). The long spermathecal duct has a simple epithelium lined internally by thin cuticle and externally thick layer muscle cells. wide lumen contains electron-dense material, among which remnants extracellular material are visible. This consists tubular structures assembled around sperm bundles previously described in male deferent ducts. so-called gland, disposed along duct, is structure with epithelial cells an irregular bearing rich system microvilli. Many mitochondria visible apical cytoplasm cells, few spheroidal bodies close to basal nuclei. Since gland suggests it involved fluid uptake from rather than secretory activity, term coined other authors this organ, inappropriate. spermatheca large complex showing duct-forming organ distinctive ultrastructural features those ducts, namely having mitochondrial matrix small crystallized area dots. Because its overall organization, D. can be considered more integrated studied hydroporine species.

Language: Английский

Contributions of Microscopy to the Morphological Characterization of the Male Genitalia of Toxomerus politus (Diptera, Syrphidae) DOI Creative Commons

Ingrid G. M. Silva,

A. A. Souza, Ariane Pandolfo Silveira

et al.

Ecology and Evolution, Journal Year: 2025, Volume and Issue: 15(2)

Published: Feb. 1, 2025

ABSTRACT The study of insects has historically been linked to the development microscopy. As techniques have improved, research into insect morphology can increasingly contribute a better understanding structures and functions morphological characters insects' three tagmata. We propose use methodologies applied in microscopy as way improving facilitating fly genitalia. In addition, flies preserved 70% alcohol were used verify feasibility distinguishing ultrastructural spermatozoa, when not previously fixed for preservation structures. shown that it is viable enhance specimens using scanning electron technique. observation genitalia this technique, together with confocal laser microscopy, enables visualization, description, characteristics also noted, analysis transmission possible recover describe some features ultrastructure Toxomerus politus (Say, 1823) sperm, obtained from stored long period time. Comparison between methods investigate T. , observed after preserving specimen, already demonstrates importance appropriate methodology starting point reliable results. adopted work potential be extrapolated other species broad context.

Language: Английский

Citations

0
Fine structure of the female genital system of diving beetle Stictonectes optatus (Seidlitz, 1887) (Dytiscidae-Hydroporinae) and evidence of mating plug formation DOI Creative Commons
Romano Dallai, David Mercati, Pietro Paolo Fanciulli

et al.

Arthropod Structure & Development, Journal Year: 2023, Volume and Issue: 73, P. 101250 - 101250

Published: March 1, 2023

The general organization of the female genital system diving beetle Stictonectes optatus was studied, clarifying complex structure spermatheca and spermathecal gland. two structures adhere closely to each other, sharing a small area their cuticular epithelium. A long duct connects bursa copulatrix spermatheca, where sperm are stored. reach common oviduct, egg fertilization occurs, via duct. gland cells have extracellular cisterns secretions Thin ducts composed duct-forming transport these apical region into lumen. Soon after mating, is almost completely occupied by plug secreted male accessory glands. epithelium seem contribute formation. Later this becomes large spherical, obstructing copulatrix.

Language: Английский

Citations

4
Proteomic diversification of spermatostyles among six species of whirligig beetles DOI Creative Commons
R. Antonio Gómez, Romano Dallai, Dylan J. Sims‐West

et al.

Molecular Reproduction and Development, Journal Year: 2024, Volume and Issue: 91(5)

Published: May 1, 2024

Abstract Seminal fluid protein composition is complex and commonly assumed to be rapidly divergent due functional interactions with both sperm the female reproductive tract (FRT), of which evolve rapidly. In addition sperm, seminal may contain structures, such as mating plugs spermatophores. Here, we investigate evolutionary diversification a lesser‐known ejaculate structure: spermatostyle, has independently arisen in several families beetles true bugs. We characterized spermatostyle proteome, FRT morphology, six species whirligig (family Gyrinidae). Spermatostyles were enriched for proteolytic enzymes, assays confirmed they possess activity. Sperm‐leucylaminopeptidases (S‐LAPs) particularly abundant, their localization spermatostyles was by immunohistochemistry. Although there evidence conservation proteomes across species, phylogenetic regressions suggest covariation between morphology FRTs. postulate that S‐LAPs (and other proteases) have evolved novel structural role discuss adaptations delivering male‐derived materials females.

Language: Английский

Citations

1
The Structure of the Female Genital System of the Diving Beetle Scarodytes halensis (Fabricius, 1787) (Hydroporinae, Dytiscidae), and the Organization of the Spermatheca and the Spermathecal Gland Complex DOI Creative Commons
Romano Dallai, David Mercati, Pietro Paolo Fanciulli

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(3), P. 282 - 282

Published: March 13, 2023

The fine structure of the female reproductive organs diving beetle Scarodytes halensis has been described, with particular attention to complex organization spermatheca and spermathecal gland. These are fused in a single whose epithelium is involved quite different activity. secretory cells gland have large extracellular cistern secretions; duct-forming cells, by their efferent duct, transport secretions up apical cell region where they discharged into lumen. On contrary, spermatheca, filled sperm, simple epithelium, apparently not ultrastructure almost identical that described closely related species Stictonectes optatus. Sc. long duct connecting bursa copulatrix spermatheca-spermathecal complex. This thick outer layer muscle cells. Through contractions, sperm can be pushed forwarding two organs. A short fertilization allows reach common oviduct eggs will fertilized. genital systems S. optatus might strategy species.

Language: Английский

Citations

3
Sperm structure of the diving beetle Deronectes moestus incospectus (Leprieur, 1876) (Hydroporinae, Dytiscidae) and considerations on extracellular material surrounding sperm bundles DOI Creative Commons
Romano Dallai, David Mercati, Pietro Paolo Fanciulli

et al.

Micron, Journal Year: 2023, Volume and Issue: 171, P. 103484 - 103484

Published: May 13, 2023

The sperm cells of the diving beetle Deronectes moestus incospectus are characterized by conjugation leading to formation bundles 64 units each. These formed at end spermatocyte cell divisions occurring in testes and can be detected anterior region deferent ducts (first type conjugation). Fusions some occur ducts. show sperm-head stacks (sperm rouleaux) surrounded a cup extracellular material secreted epithelial This extends posteriorly around bundle cover nuclei initial flagella. consists fine tubules, is no longer visible posterior D. have an axoneme with 9 + 2 pattern unusual mitochondrial derivatives having matrix showing dense dots small crystallized domain. Two thin elongated accessory bodies located between axoneme. different morphologies various families Adephaga, but all produced epithelium Thus it reasonable assume that has same function groups.

Language: Английский

Citations

3
Morphology of the Female and Male Reproductive Tracts and More Data on the Spermatostyle in the Brazilian Gyretes sp. (Coleoptera, Adephaga, Gyrinidae) DOI
Karen Salazar,

Ademária Moreira Novais,

José Lino‐Neto

et al.

Microscopy and Microanalysis, Journal Year: 2023, Volume and Issue: 29(6), P. 2184 - 2203

Published: Nov. 22, 2023

We investigated the male and female reproductive tracts of Gyretes sp. with light transmission electron microscopies. The has a pair testes single coiled follicle, followed by short efferent ducts, which have similar shape diameter to testes. Long ducts (epididymides) differential epithelium open in long vasa deferentia that lead accessory glands. Glycoprotein secretions from vas deferens constitute spermatostyle for spermatozoa aggregation. numerous ovarioles per ovary, fertilization duct, an gland, elongated vagina. Spermatozoa are stored as unaggregated cells duct. In Gyrinidae, glands show diverse shapes, sperm storage organs vary shape, size, type may play role interaction aggregates. Testes follicle opening features shared other Gyrinidae Adephaga. proposed adding this latter trait characterize suborder beetles. morphology both sexes contributes comparative analyses knowledge biology provide additional systematics.

Language: Английский

Citations

1
The Cap and the Spermatostyle Protecting the Sperm Bundle Have a Similar Origin—Ultrastructural Study of the Spermatogenesis from the Ground Beetle Carabus (Chaetocarabus) lefebvrei Dejean, 1826 (Adephaga Carabidae) DOI Creative Commons
Pietro Lupetti, David Mercati, Anita Giglio

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(11), P. 864 - 864

Published: Nov. 5, 2024

The males of

Language: Английский

Citations

0
Ultrastructure of the Female Reproductive Organs of the Diving Beetle Deronectes Moestus Incospectus (Leprieur, 1876) (Dytiscidae, Hydroporinae) DOI
Romano Dallai, David Mercati, Paulo Henrique Rezende

et al.

Published: Jan. 1, 2023

We describe the ultrastructure of female reproductive organs Deronectes moestus (Dytiscidae Hydroporinae). The long spermathecal duct has a simple epithelium lined with thin cuticle and surrounded by thick layer muscle fibres. wide lumen contains electron-dense secretory material, among which remnants extracellular material are visible. This consists tubular structures assembled around sperm bundles previously described in male deferent ducts. so-called gland, disposed along duct, is structure epithelial cells an irregular bearing rich system microvilli. Many mitochondria visible apical cytoplasm cells, few spheroidal bodies close to basal nuclei. Since gland suggests it involved fluid uptake from rather than activity, term coined other authors this organ, inappropriate. spermatheca large complex showing duct-forming cells. organ distinctive ultrastructural features those ducts, namely having mitochondrial matrix small crystallized area dots. Because its overall organization, D. can be considered more integrated studied hydroporine species.

Language: Английский

Citations

0
Ultrastructure of the female reproductive organs of the diving beetle Deronectes moestus incospectus (Leprieur, 1876) (Dytiscidae, Hydroporinae) DOI Creative Commons
Romano Dallai, David Mercati, Paulo Henrique Rezende

et al.

Arthropod Structure & Development, Journal Year: 2023, Volume and Issue: 75, P. 101287 - 101287

Published: July 1, 2023

We describe the ultrastructure of female reproductive organs Deronectes moestus (Dytiscidae Hydroporinae). The long spermathecal duct has a simple epithelium lined internally by thin cuticle and externally thick layer muscle cells. wide lumen contains electron-dense material, among which remnants extracellular material are visible. This consists tubular structures assembled around sperm bundles previously described in male deferent ducts. so-called gland, disposed along duct, is structure with epithelial cells an irregular bearing rich system microvilli. Many mitochondria visible apical cytoplasm cells, few spheroidal bodies close to basal nuclei. Since gland suggests it involved fluid uptake from rather than secretory activity, term coined other authors this organ, inappropriate. spermatheca large complex showing duct-forming organ distinctive ultrastructural features those ducts, namely having mitochondrial matrix small crystallized area dots. Because its overall organization, D. can be considered more integrated studied hydroporine species.

Language: Английский

Citations

0