Comprehensive analysis of plasma cell heterogeneity and immune interactions in multiple myeloma

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 22, 2025

This study focused on the role of plasma cells in multiple myeloma (MM) and associated potential mechanisms. Transcriptomic data MM various gene sets from several public databases were downloaded for subsequent analyses. Through single-cell sequencing, 10 major cell types identified annotated. The differential expression pathway enrichment between different subtypes as well communication analysis, transcriptional regulation analysis conjunction with malignant subpopulation performed. Next, samples clustered into two groups by applying non-negative matrix factorization (NMF). Additional revealed notable disparities survival clusters, correlation genes involved classical metabolic pathways dysregulation, thus confirming stability validity clustering. Subsequently, Weighted Gene Co-expression Network Analysis was performed hub modules most strongly clustering extracted. We then constructed a prognostic prediction model using Least Absolute Shrinkage Selection Operator multiCox regression analysis. predictive accuracy evaluated robustness confirmed separate validation cohort. dysregulation risk analyzed. Ultimately, an investigation conducted association immunological features, terms antitumor immunotherapy, tumor microenvironment, immune checkpoints. provides in-depth mechanisms underlying development offers new directions to improve therapeutic approaches enhance patient outcomes.

Language: Английский

Lipid levels and multiple myeloma risk: insights from Meta-analysis and mendelian randomization

Lipids in Health and Disease, Journal Year: 2024, Volume and Issue: 23(1)

Published: Sept. 16, 2024

Lipid levels have been suggset to be correlated with multiple myeloma (MM) risk, though causality remains unconfirmed. To explore this further, a detailed study combining meta-analysis and Mendelian randomization (MR) was conducted.

Language: Английский