Assembly of neuron- and radial glial cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons DOI Creative Commons

Ayumu Mubuchi,

Mina Takechi,

Shunsuke Nishio

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Nov. 9, 2023

Radial neuronal migration is a key neurodevelopmental event for proper cortical laminar organization. The multipolar-to-bipolar transition, critical step in establishing polarity during radial migration, occurs the subplate/intermediate zone (SP/IZ), distinct region of embryonic cerebral cortex. It has been known that extracellular matrix (ECM) molecules are enriched SP/IZ. However, molecular constitution and functions ECM formed this remain poorly understood. Here, we identified neurocan (NCAN) as major chondroitin sulfate proteoglycan mouse NCAN binds to both glial-cell-derived tenascin-C (TNC) hyaluronan (HA), large linear polysaccharide, forming ternary complex NCAN, TNC, HA Developing neurons make contact with migration. enzymatic or genetic disruption impairs by suppressing transition. Furthermore, TNC promoted morphological maturation vitro. present results provide evidence cooperative role neuron- development.

Language: Английский

Abnormal morphology and synaptogenic signaling in astrocytes following prenatal opioid exposure DOI Creative Commons

Ethan B. Niebergall,

Daron Weekley,

Anna Mazur

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 11, 2024

Abstract In recent decades, there has been a dramatic rise in the rates of children being born after utero exposure to drugs abuse, particularly opioids. Opioids have shown detrimental effects on neurons and glia central nervous system (CNS), but impact prenatal opioid (POE) still-developing synaptic circuitry is largely unknown. Astrocytes exert strong influence development, secreting factors that both promote inhibit synapse formation neuronal maturation developing CNS. Here, we investigated partial µ-opioid receptor agonist, buprenorphine, astrocyte synaptogenic signaling morphological development cortical cell culture. Acute buprenorphine treatment had no effect excitatory number astrocyte-free neuron cultures. conditions where shared culture media with astrocytes, attenuated capabilities astrocyte-secreted factors. Neurons cultured from drug-naïve mice showed change synapses when treated secreted by astrocytes POE mice. However, this same was applied mice, suggestive complex neuroadaptive response maintains pathways face impaired signaling. addition promoting connectivity changes neurons, exerted disrupting their structural accumulation lipid droplets (LD), maladaptive stress system.

Language: Английский

Citations

0
Glia in inhibitory synaptogenesis DOI

Leslie K. Ferrarelli

Science Signaling, Journal Year: 2024, Volume and Issue: 17(838)

Published: May 28, 2024

Astrocyte-secreted neurocan guides the formation of inhibitory circuits in brain.

Language: Английский

Citations

0
Developmental and Age-Related Synapse Elimination is Mediated by Glial Croquemort DOI
Taylor R. Jay, Yunsik Kang,

Victor Ouellet-Massicotte

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Pramipexole, a D3 receptor agonist, increases cortical gamma power and biochemical correlates of cortical excitation; implications for mood disorders DOI

Karli F. Gilbert,

Matthew Amontree,

Samantha Deasy

et al.

European Journal of Neuroscience, Journal Year: 2024, Volume and Issue: 60(10), P. 6490 - 6508

Published: Oct. 16, 2024

Major depressive disorder (MDD) has been associated with deficits in working memory as well underlying gamma oscillation power. Consistent this, overall reductions cortical excitation have also described MDD. In previous work, we demonstrated that the monoamine reuptake inhibitor venlafaxine increases power ex vivo hippocampal slices and this is concomitant pyramidal arbour reduced levels of plasticity-restricting perineuronal nets (PNNs). present study, examined effects chronic treatment pramipexole (PPX), a D3 dopamine receptor agonist, for its on measured by electroencephalography (EEG) recordings female BALB/c C57Bl6 mice. We observe modest but significant increase 20-50 Hz PPX both strains. Additionally, biochemical analysis prefrontal cortex lysates from PPX-treated mice shows number changes could contribute to, or follow from, increased excitability and/or PPX-associated include specific PNN components tissue matrix metalloproteases-1 (TIMP-1), which inhibits long-term potentiation synaptic transmission. power, proteins TIMP-1, improves reduces anhedonia. Together these results add to an emerging literature linking extracellular mood cognition.

Language: Английский

Citations

0
Assembly of neuron- and radial glial cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons DOI Creative Commons

Ayumu Mubuchi,

Mina Takechi,

Shunsuke Nishio

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Nov. 9, 2023

Radial neuronal migration is a key neurodevelopmental event for proper cortical laminar organization. The multipolar-to-bipolar transition, critical step in establishing polarity during radial migration, occurs the subplate/intermediate zone (SP/IZ), distinct region of embryonic cerebral cortex. It has been known that extracellular matrix (ECM) molecules are enriched SP/IZ. However, molecular constitution and functions ECM formed this remain poorly understood. Here, we identified neurocan (NCAN) as major chondroitin sulfate proteoglycan mouse NCAN binds to both glial-cell-derived tenascin-C (TNC) hyaluronan (HA), large linear polysaccharide, forming ternary complex NCAN, TNC, HA Developing neurons make contact with migration. enzymatic or genetic disruption impairs by suppressing transition. Furthermore, TNC promoted morphological maturation vitro. present results provide evidence cooperative role neuron- development.

Language: Английский

Citations

0