Poultry Science,
Journal Year:
2024,
Volume and Issue:
103(10), P. 104145 - 104145
Published: July 30, 2024
Mycoplasma
gallisepticum
(MG)
is
a
pathogen
that
induces
chronic
respiratory
illnesses
in
chickens,
leading
to
tracheal
and
lung
injury,
eliciting
immune
reactions
support
sustained
colonization.
Baicalin,
compound
found
scutellaria
baicalensis,
exhibits
anti-inflammatory,
antioxidant,
antibacterial
properties.
This
study
aimed
investigate
the
potential
of
baicalin
alleviating
cell
damage
caused
by
MG
restoring
imbalances
M1/M2
Th1/Th2
differentiation
explore
its
underlying
mechanism.
In
this
research,
model
for
polarization
induced
was
initially
developed.
Specifically,
infection
with
at
multiplicity
(MOI)
400
6
h
represented
M1
model,
while
10
M2
model.
The
markers
were
subsequently
validated
using
qRT-PCR,
ELISA,
Western
blot
analysis.
Baicalin
disrupts
activation
cells
has
restore
balance
between
cells,
thereby
mitigating
inflammatory
resulting
from
MG.
Subsequent
studies
on
MG-infected
chickens
detected
alveolar
lavage
fluid,
as
well
macrophages
Th
lung.
M1/Th1
exposed
5
d,
M2/Th2
infected
7
d.
utilization
both
light
electron
transmission
microscopes
revealed
administration
resulted
reduction
number
decrease
cytoplasmic
vacuoles,
restoration
mitochondrial
swelling
chromatin
agglutination,
alleviation
rupture
infiltration.
Furthermore,
restored
MG-induced
inhibited
phosphorylation
p38
p65
proteins,
hindering
TLR4-p38
MAPK/NF-κB
pathway.
provides
insights
into
long-term
effects
offers
theoretical
basis
practical
applications.
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 20, 2025
Abstract
Herpes
simplex
virus
type
1
(HSV‐1)
is
a
prevalent
human
pathogen
primarily
transmitted
through
skin‐to‐skin
contact.
Traditional
antiviral
drugs
like
acyclovir
(ACV)
have
limitations
due
to
viral
resistance
and
side
effects,
necessitating
the
development
of
alternative
therapeutic
strategies.
Drug‐loaded
hydrogels
emerged
as
promising
approach
for
managing
various
skin
infections.
Considering
low‐pH
microenvironment
following
HSV‐1
infection,
pH‐responsive
baicalein@chitosan
(B@C)
hydrogel
developed
topical
treatment
This
synthesized
by
incorporating
baicalein,
natural
flavonoid,
into
chitosan
matrix
modified
with
4‐formylphenylboronic
acid
protocatechualdehyde
achieve
potent
anti‐HSV‐1
activity
pH‐responsiveness.
In
vitro
results
demonstrated
hydrogel's
pH‐dependent
inhibitory
effect
on
infections,
including
ACV‐resistant
strains.
Subsequent
investigations
confirmed
its
efficacy
in
multiple
murine
infection
models.
Mechanistically,
B@C
inhibited
replication
modulating
phosphorylation
inhibitor
nuclear
factor
kappa‐B
kinase
subunit
beta,
promoted
collagen
synthesis,
decreased
reactive
oxygen
species
generation.
Ultra‐high‐performance
liquid
chromatography‐tandem
mass
spectrometry
analysis
revealed
sustained
release
baicalein
from
hydrogel,
ensuring
long‐term
drug
retention
HSV‐1‐infected
tissues.
Collectively,
these
findings
suggest
that
holds
significant
potential
management
Journal of Food Safety,
Journal Year:
2025,
Volume and Issue:
45(1)
Published: Feb. 1, 2025
ABSTRACT
Foodborne
pathogens
are
critical
concerns
in
the
food
safety
sector
and
for
regulatory
agencies
due
to
their
high
morbidity
mortality
rates.
They
also
significantly
impact
economy,
particularly
countries
with
limited
financial
resources.
Furthermore,
rapid
progression
of
microbial
resistance
antibiotics
hazards
associated
chemical
preservatives
global
challenges.
This
underscores
importance
developing
natural
antimicrobial
additives
industry.
Plants
offer
a
promising
source
effective
agents
significant
activity
against
foodborne
pathogens.
In
particular,
phytochemicals
demonstrate
potential
improving
broad
properties.
this
context,
we
reviewed
classification
extraction
processes
phytochemicals,
activities,
mechanisms
action,
factors
influencing
efficacy,
applications
safety.
Additionally,
explored
current
challenges
industrial
application
as
preservatives.
Phytochemicals
have
demonstrated
substantial
effects
variety
through
diverse
mechanisms.
Thus,
they
represent
solution
advancing
industry
enhancing
