Microbial antibiotic resistance genes across an anthropogenic gradient in a Canadian High Arctic watershed DOI Creative Commons

Juliette Provencher,

Paul B. L. George, Mary Thaler

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1)

Published: Jan. 1, 2024

Abstract Antibiotic resistance is one of the biggest challenges to public health. While discovery antibiotics has decreased pathogen-caused mortality, overuse these drugs resulted in increased transfer and evolution antibiotic genes (ARGs) bacteria. ARGs naturally occur wild bacterial communities, but are also found concentrations environments contaminated by wastewater effluent. Although such relatively well described temperate environments, little known about distribution dissemination Arctic. We characterized microbial communities from aerosols, lakes mats around a remote Arctic hamlet using metagenomic approaches. Specific objectives were (i) compare across habitats, (ii) characterize ARG populations along continuum anthropogenically influenced (iii) identify viral origin. identified all habitats throughout watershed, that most impacted area had highest diversity relative uncontaminated sites, which may be remnant signal effluent inputs during 20th century. we predominantly genomes, our data suggests mimiviruses harbor ARGs.

Language: Английский

Unraveling the multiple interactions between phages, microbes and flavor in the fermentation of strong-flavor Baijiu DOI Creative Commons

Huadong Zhang,

Hongxia Zhang, Hai Du

et al.

Bioresources and Bioprocessing, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 5, 2025

The fermentation process of strong-flavor Baijiu represents a complex and unique ecosystem, characterized by the involvement various microorganisms that drive intricate biochemical reactions, ultimately contributing to distinct flavor profile Baijiu. Viruses may affect succession thus style quality product. However, interaction between viruses during is still unclear. Here we combined viral metagenomics amplicon sequencing, physicochemical analysis, GC-MS detection with temporal sampling study dynamics microbial communities, properties, compounds fermentation. Viral metagenomic analysis revealed 513 operational taxonomic units (vOTUs), encompassing 34 families. Principal coordinates (PCoA) demonstrated significant differences in vOTUs at different stages. Notably, community exhibited patterns stages; it changed rapidly initial five days, similarities observed days 10 20. Volatile identified 38 components fermented grains, comprising 16 ester compounds, 11 alcohols, 8 acids, majority formed 30. Spearman's rank correlation Peduoviridae negative Gluconobacter. Genomoviridae showed Issatchenkia, Penicillium, Monascus. These findings highlight potential for interactions communities fermentation, underscoring importance considering studies ecology foods.

Language: Английский

Citations

1
Giant viral signatures on the Greenland ice sheet DOI Creative Commons

Laura Perini,

Katie Sipes, Athanasios Zervas

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: May 17, 2024

Abstract Background Dark pigmented snow and glacier ice algae on glaciers sheets contribute to accelerating melt. The biological controls these algae, particularly the role of viruses, remain poorly understood. Giant classified under nucleocytoplasmic large DNA viruses (NCLDV) supergroup (phylum Nucleocytoviricota), are diverse globally distributed. NCLDVs known infect eukaryotic cells in marine freshwater environments, providing a control algal population ecosystems. However, there is very limited information diversity ecosystem function terrestrial icy habitats. Results In this study, we investigate for first time giant their host connections habitats, such as cryoconite, dark ice, core, red green snow, genomic assemblies five cultivated Chlorophyta algae. virus marker genes were present almost all samples; highest abundances recovered from assemblies, followed by ice. variety active protists GrIS habitats containing NCLDV suggests that infection can occur range hosts. Metagenomic data contained evidence metagenome-assembled genomes orders Imitervirales, Asfuvirales, Algavirales. Conclusion Our study highlights family signatures samples Greenland sheet. (GVMAGs) found samples, related identified culture assemblies; implying relationship between Metatranscriptomic viral also aligned with metagenomic sequences, suggesting an component microbial community potential “top-down” protistan members. This reveals unprecedented presence glacial dominated

Language: Английский

Citations

8
Glacier-preserved Tibetan Plateau viral community probably linked to warm–cold climate variations DOI
Zhi-Ping Zhong, Olivier Zablocki,

Yueh-Fen Li

et al.

Nature Geoscience, Journal Year: 2024, Volume and Issue: 17(9), P. 912 - 919

Published: Aug. 26, 2024

Language: Английский

Citations

5
The diversity and risk of potential pathogenic bacteria on the surface of glaciers in the southeastern Tibetan Plateau DOI
Binglin Zhang,

Yeteng Xu,

Xiao Yan

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 945, P. 173937 - 173937

Published: June 15, 2024

Language: Английский

Citations

4
Glacial microbial-environmental interactions on the Tibetan Plateau: a review DOI Creative Commons

Jiajie Xu,

Jing Zhu, Yonghong Zhou

et al.

Water Biology and Security, Journal Year: 2025, Volume and Issue: unknown, P. 100366 - 100366

Published: Jan. 1, 2025

Language: Английский

Citations

0
Distance to the water table shapes the diversity and activity of DNA and RNA viruses in a subalpine peatland DOI Creative Commons
Z. Xiong, Xuan Qiu, Xing Xiang

et al.

Environment International, Journal Year: 2025, Volume and Issue: 197, P. 109363 - 109363

Published: March 1, 2025

Peatlands are essential reservoirs of carbon and critical zones for the cycling greenhouse gases on Earth. Their ecological functions primarily governed by microbial communities inhabiting them, which vary with hydrological conditions. However, roles viruses in peatland ecosystems remain poorly understood despite their abundance ubiquity. To address this gap, viral communities, roles, responses to environmental factors were explored using viromics, metatranscriptomics, physicochemical property analyses nine peat sediments collected from various layers three profiles different water table levels Dajiuhu Peatland, central China. This study revealed that distance (DWT) significantly influenced composition function altering redox potential total organic carbon, turn affected methane (CH4) concentrations pore water. Furthermore, a notable putative auxiliary metabolic genes associated methane, nitrogen, sulfur metabolism was identified DNA viruses, community strongly regulated DWT. Additionally, functional related oxidative phosphorylation cysteine synthesis detected first time RNA viruses. advances our comprehension how conditions affect peatlands, provides new insights into impact CH4 cycle, serves as crucial reference future investigations

Language: Английский

Citations

0
Genomes of Prochlorococcus, Synechococcus, bacteria, and viruses recovered from marine picocyanobacteria cultures based on Illumina and Qitan nanopore sequencing DOI Creative Commons
Qingtao Wu, Jie Gao,

Boxuan Sa

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: April 12, 2025

Prochlorococcus and Synechococcus are key contributors to marine primary production play essential roles in global biogeochemical cycles. Despite the ecological importance of these two picocyanobacterial genera, current genomic datasets still lack comprehensive representation under-sampled ocean regions, associated bacteria viruses. To address this gap, we used a combination second- third-generation sequencing technologies assemble data from 105 Picocyanobacterial enrichment cultures isolated Indian Ocean, South China Sea, western Pacific Ocean. This dataset includes 55 50 genomes with high completeness (>98%) low contamination (<2%), along 308 non-redundant bacterial derived 1,457 medium- high-quality non-cyanobacteria metagenome-assembled (MAGs, ≥50% ≤10%). Additionally, 2,113 viral operational taxonomic units (vOTUs) were total 7632 qualified contigs. provides valuable resource for improving our understanding complex interactions among Prochlorococcus, Synechococcus, their viruses ecosystems, offering foundation study evolutionary dynamics.

Language: Английский

Citations

0
Review of Methods for Studying Viruses in the Environment and Organisms DOI Creative Commons
Xinyue Wang, Tianjiao Ma, Zhiyuan Chen

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(1), P. 86 - 86

Published: Jan. 11, 2025

Recent decades have seen growing attention on viruses in the environment and their potential impacts as a result of global epidemics. Due to diversity viral species along with complexity environmental host factors, virus extraction detection methods become key for study ecology. This review systematically summarises extracting detecting pathogens from different samples (e.g., soil, water, faeces, air) biological plants, animals) existing studies, comparing similarities differences, applicability, well advantages disadvantages each method. Additionally, this discusses future directions research field. The aim is provide theoretical foundation technical reference ecology research, facilitating further exploration applications

Language: Английский

Citations

0
Bioclimatic zonation and spatial-scale dependence of lacustrine microbial assemblages DOI
Shuren Wang, Qinglong L. Wu, Huabing Li

et al.

Science Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Emerging Trends in Antimicrobial Resistance in Polar Aquatic Ecosystems DOI Creative Commons
Melissa Bisaccia, Francesca Berini, Flavia Marinelli

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(4), P. 394 - 394

Published: April 10, 2025

The global spread of antimicrobial resistance (AMR) threatens to plummet society back the pre-antibiotic era through a resurgence common everyday infections’ morbidity. Thus, studies investigating antibiotic genes (ARGs) and antibiotic-resistant bacteria (ARB) in urban, agricultural, clinical settings, as well extreme environments, have become increasingly relevant One Health perspective. Since Antarctic Arctic regions are considered amongst few remaining pristine environments on Earth, characterization their native resistome appears be utmost importance understand whether how it is evolving result anthropogenic activities climate change. In present review, we report phenotypic (e.g., disk diffusion test) genotypic PCR, metagenomics) approaches used study AMR aquatic environment polar regions, water represents one main dissemination routes nature. Their advantages limits described, emerging trends resulting from analysis ARB ARGs waters discussed. detected these mostly comparable those more anthropized areas, with predominance tetracycline, β-lactam, sulfonamide (and related ARGs). Indeed, is, all cases, consistently highlighted sites impacted by human wildlife respect ones. Surprisingly, aminoglycoside fluroquinolone determinants seem an even higher incidence compared that other areas world, corroborating need for thorough surveillance regions.

Language: Английский

Citations

0