Evaluation of Environmental DNA as a Surveillance Tool for Invasive House Mice (Mus musculus)

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 1, 2025

ABSTRACT Increasing the success of invasive species management depends on development, testing, and deployment new tools. Environmental DNA (eDNA) is an effective tool for monitoring that can help identify presence/absence, geographical boundaries invasion, risk pathways, population connectivity. In particular, understanding sensitivity eDNA detection rates to target density allows calibration sampling rates. this study, we take a lab‐validated assay Mus musculus (house mouse) test its at different populations densities wild‐caught, free‐ranging M. in controlled laboratory outdoor mesocosm. The goal was understand both accumulation after introduced persistence accumulated signal environment animals were removed. We found detectable within 1 h single mouse being months but largely undetectable 4 days suggest strategies post‐eradication highlight other uses assay, which are important management.

Language: Английский

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How cryptic animal vectors of fungi can influence forest health in a changing climate and how to anticipate them

Applied Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 109(1)

Published: March 15, 2025

Abstract Fungal spores are usually dispersed by wind, water, and animal vectors. Climate change is accelerating the spread of pathogens to new regions. While well-studied vectors like bark beetles moths contribute pathogen transmission, other, less-recognized species play a crucial role at different scales. Small-scale dispersers, such as mites, rodents, squirrels, woodpeckers, facilitate fungal within trees or entire forest On larger scale, birds significantly long-distance dispersal, potentially aiding establishment invasive across continents. These remain underexplored often overlooked in disease studies therefore called cryptic Understanding full range dispersal mechanisms critical climate drive shifts distributions increases vector activity. Expanding monitoring detection tools include these hidden carriers will improve our ability track distribution pathogens. Integrating targeted research, innovative technologies, collaborative efforts disciplines borders essential for enhancing management mitigating disease’s ecological economic impacts. Key points • Cryptic spore forests accelerates altering distributions, increasing activity, facilitating dispersal. Innovative tools, eDNA sampling predictive modelling, uncover contributions mitigate

Language: Английский

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What you eat is what we need: using ants to detect spotted lanternfly (Lycorma delicatula) DNA

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract BACKGROUND Early detection of invasive species such as the spotted lanternfly (SLF, Lycorma delicatula ) is critical for effective management including eradication efforts and limiting further spread. SLF excretes honeydew containing detectable DNA, providing a unique opportunity to leverage environmental DNA (eDNA) its detection. This study introduces ant‐derived (antDNA) approach, utilizing ants ‘honeydew samplers’ detect DNA. We validated effectiveness this method through three experiments. RESULTS Using SLF‐specific polymerase chain reaction (PCR), we consistently detected in foraging or nesting near infestations. then showed that after single meal, persisted at least 5 days, even when, subsequently, were fed plain honey solution. Lastly, collected from honey‐baited lure stations along transects radiating infestations yielded positive detections up 100 m core infestations, demonstrating method's extensive range. These findings confirm ants, their active feeding on ability retain ingested material, are highly reliable samplers. CONCLUSION Combined with ants' ecological dominance ease low cost ant collection, antDNA offers sensitive, efficient practical alternative traditional, labor‐intensive approaches detecting potentially other honeydew‐producing insects. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

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Safeguarding Iguana diversity: enabling rapid and low-effort tracking of non-native iguanas through terrestrial eDNA innovations

Biological Invasions, Journal Year: 2025, Volume and Issue: 27(1)

Published: Jan. 1, 2025

Language: Английский

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Environmental DNA as a tool for soil health monitoring and unveiling new ecological frontiers

Ecological Indicators, Journal Year: 2025, Volume and Issue: 174, P. 113438 - 113438

Published: April 9, 2025

Language: Английский

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Plant biosecurity and One Health: government and industry roles as risk creators and mitigators

One Health Outlook, Journal Year: 2025, Volume and Issue: 7(1)

Published: April 25, 2025

Language: Английский

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Environmental DNA Methods for Detection of Varroa destructor in Honey Bee (Apis mellifera) Hives

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(3)

Published: May 1, 2025

ABSTRACT The parasitic mite, Varroa destructor , is a worldwide problem for honey bees ( Apis mellifera ). recent spread of this pest to Australia, which was one the few varroa‐free bee populations remaining, highlights importance effective biosecurity methods managing incursions. Detection varroa mite environmental DNA (eDNA) could be valuable complementary tool increase sensitivity and cost‐effectiveness surveillance as it spreads new areas. Using newly developed V. quantitative PCR assay, we deployed eDNA testing on hives in New Zealand Australia with range infestation levels. detection using hive surface swabs comparable conventional alcohol wash method used by beekeepers, advantage not harming bees. However, observed greater variability detection, particularly entrance swabs, when estimated levels were below 1%. introduced from Chatham Islands Wellington, Zealand, also able show that detectable during initial invasion hives. Our data highlight potential incorporating into Australia's programs help limit mites areas detect future

Language: Английский

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CRISPR‐Based Environmental Biosurveillance Assisted via Artificial Intelligence Design of Guide‐RNAs

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(3)

Published: May 1, 2025

ABSTRACT Environmental biosecurity challenges are intensifying as climate change and human activities accelerate the spread of invasive species, disrupting ecosystem composition, function, essential services. DNA (eDNA) has transformed traditional biosurveillance by detecting trace fragments left organisms in their surroundings, primarily applying quantitative polymerase chain reaction (qPCR) methods. However, qPCR presents challenges, including limited portability, reliance on precise thermal cycling, susceptibility to inhibitors. To address these enable field‐deployable monitoring, isothermal amplification techniques such recombinase (RPA) paired with clustered regularly interspaced short palindromic repeats associated proteins (CRISPR‐Cas) have been proposed promising alternatives. CRISPR‐Cas technology also searching optimizing a guide RNA (gRNA) that is highly sensitive no off‐target interactions for use an effective environmental tool. We present here development SENTINEL ( S mart E nvironmental N ucleic‐acid T racking using I nference from eural‐networks arly‐warning L ocalization) harnesses programmability, specificity sensitivity one‐pot RPA‐CRISPR‐Cas13a integrating accessible pre‐trained neural network assay design rapid deployment. challenged waterborne eDNA two marine sites invaded species not native New Zealand proof‐of‐concept fluorescence‐based tests: Sabella spallanzanii (Mediterranean fanworm) Undaria pinnatifida (Wakame). Off‐target effects were explored challenging assays gDNA suite co‐occurring species. presented robust, streamlined method incorporating trained network, achieving down 10 attomolar recombinant ~0.34 copies/μL samples 1 h, costing 3.5 USD per sample. There was 100% agreement between results qPCR‐based analysis samples. displayed activity when against 23 Thus, our study showcases SENTINEL's potential robust platform screening applications.

Language: Английский

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Macrobial airborne environmental DNA analysis: A review of progress, challenges, and recommendations for an emerging application

Molecular Ecology Resources, Journal Year: 2024, Volume and Issue: 24(7)

Published: Aug. 8, 2024

In the context of looming global biodiversity loss, effective species detection represents a critical concern for ecological research and management. Environmental DNA (eDNA) analysis, which refers to collection taxonomic identification genetic fragments that are shed from an organism into its surroundings, emerged approximately 15 years ago as sensitive tool detection. Today, one frontiers eDNA concerns analysis material in dust other airborne materials, termed analysis. As study matures, it is appropriate time review foundational emerging studies make up current literature, use reviewed literature summarize, synthesize, forecast major challenges opportunities this advancing front. Specifically, we "ecology eDNA" framework organize our findings across origin, state, transport, fate materials environment, summarize what so far known their interactions with surrounding abiotic biotic factors, including population community ecologies ecosystem processes. Within work identify key challenges, opportunities, future directions associated application development. Lastly, discuss development applications, partnerships, messaging promote growth field. Together, broad potential rate at accelerating field suggest sky's limit science.

Language: Английский

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Soil eDNA biomonitoring reveals changes in multitrophic biodiversity and ecological health of agroecosystems

Environmental Research, Journal Year: 2024, Volume and Issue: 262, P. 119931 - 119931

Published: Sept. 10, 2024

Language: Английский

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