Size-Dependent Cytotoxicity and Multi-Omic Changes Induced by Amorphous Silicon Nanoparticles in HepG2 Cells

Toxics, Journal Year: 2025, Volume and Issue: 13(4), P. 232 - 232

Published: March 21, 2025

(1) Background: Silica nanoparticles (SiO2 NPs) have a high potential for human exposure and tend to accumulate in the liver. This study aimed explore size-dependent cytotoxicity induced by SiO2 NPs identify key molecular pathways at vitro level through proteomics, metabolomics, combination of multiple omics methods. (2) Methods: The hepatoma cells (HepG2) were exposed three different sizes (60, 250, 400 nm) doses 0, 12.5, 25, 50, 100, 200 μg/mL 24 h. (3) Results: Exposure 60 nm more reduction cell viability than other two larger-scale particles. Changes metabolomic proteomic profiles HepG2 also size-dependent. main that significantly affected treatment group represented cholesterol metabolism proteomics central carbon metabolomics. Moreover, common enrichment between differential proteins metabolites included protein digestion absorption vitamin absorption. (4) Conclusions: could induce changes probably mainly interfering with energy pathways.

Language: Английский

Metabolomic and molecular analysis reveals multiple pathways of TBBPA-induced developmental toxicity in zebrafish embryos

Toxicology and Applied Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 117295 - 117295

Published: March 1, 2025

Language: Английский