Development of human iPS cell-derived alveolar and airway models using micropatterning plate for SARS-CoV-2 research DOI Open Access
Rina Hashimoto,

Atsushi Masui,

Shimpei Gotoh

et al.

Uirusu, Journal Year: 2024, Volume and Issue: 74(1), P. 35 - 44

Published: Jan. 1, 2024

Advanced cell culture systems including human induced pluripotent stem (iPS) cells and organoids enable the generation of intricate structural functional organ models in vitro. Application these advanced to research on a wide range diseases infectious is underway. Due impact coronavirus disease 2019 (COVID-19) pandemic, virus field are rapidly becoming popular. Respiratory generated using iPS organoid technology useful for analyzing respiratory responses caused by severe acute syndrome 2 (SARS-CoV-2) infection. However, there still room development an apical-out model, which essential simple infection experiments, model that can analyze host alveoli airways. In this study, we developed cell-derived alveolar airway with structure micropatterning plate. confirmed contains abundant type II epithelial (AT2) cells, target SARS-CoV-2 alveoli. ciliated airway. Using our models, differences efficiency response each variant. We hope plate will be used not only but also viruses.

Language: Английский

Micro-Physiological System of Human Lung: The Current Status and Application to Drug Discovery DOI Creative Commons
Naoyuki Sone, Shimpei Gotoh

Drug Metabolism and Pharmacokinetics, Journal Year: 2025, Volume and Issue: 60, P. 101050 - 101050

Published: Jan. 9, 2025

Various attempts have been made to elucidate the mechanisms of human lung development, its physiological functions, and diseases, in hope new drug discovery. Recent technological advancements experimental animals, cell culture, gene editing, analytical methods provided insights therapeutic strategies. However, results obtained from animal experiments are often inconsistent with those data because reproducibility issues caused by structural differences between mice humans. In addition, it is not possible accurately reproduce internal environment structure using conventional two-dimensional (2D) or three-dimensional (3D) culture methods. As a result, micro-physiological system (MPS) technology, such as "lung-on-a-chip" that can cells state close body developed, applications disease models, toxicological studies, discovery accelerated worldwide. Here, we focus on mimetics lung, including review their recent progress, achievements challenges. Finally, discuss role these chips for refractory diseases.

Language: Английский

Citations

0
Modèles précliniques in vitro reproduisant l’épithélium respiratoire : application à l’étude de l’infection par le virus SARS-CoV-2 DOI

L. Morichon,

S. Assou,

A. Bourdin

et al.

Revue des Maladies Respiratoires, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Stably-Inverted Apical-Out Human Upper Airway Organoids for SARS-CoV-2 Infection and Therapeutic Testing DOI Creative Commons

Ji‐Hoon Lee,

Julia C. LeCher, Eric Parigoris

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 4, 2024

While breakthroughs with organoids have emerged as next-generation

Language: Английский

Citations

0
Development of human iPS cell-derived alveolar and airway models using micropatterning plate for SARS-CoV-2 research DOI Open Access
Rina Hashimoto,

Atsushi Masui,

Shimpei Gotoh

et al.

Uirusu, Journal Year: 2024, Volume and Issue: 74(1), P. 35 - 44

Published: Jan. 1, 2024

Advanced cell culture systems including human induced pluripotent stem (iPS) cells and organoids enable the generation of intricate structural functional organ models in vitro. Application these advanced to research on a wide range diseases infectious is underway. Due impact coronavirus disease 2019 (COVID-19) pandemic, virus field are rapidly becoming popular. Respiratory generated using iPS organoid technology useful for analyzing respiratory responses caused by severe acute syndrome 2 (SARS-CoV-2) infection. However, there still room development an apical-out model, which essential simple infection experiments, model that can analyze host alveoli airways. In this study, we developed cell-derived alveolar airway with structure micropatterning plate. confirmed contains abundant type II epithelial (AT2) cells, target SARS-CoV-2 alveoli. ciliated airway. Using our models, differences efficiency response each variant. We hope plate will be used not only but also viruses.

Language: Английский

Citations

0