Soft Matter, Journal Year: 2024, Volume and Issue: 20(45), P. 8947 - 8951
Published: Jan. 1, 2024
We show the assembly and cross-linking of carboxylate-modified polystyrene nanoparticles into flexible circular, ring-shaped structures with micrometer sized diameters around base surface-adhered giant lipid vesicles.
Language: Английский
Trends in Biochemical Sciences, Journal Year: 2024, Volume and Issue: 49(9), P. 829 - 840
Published: June 29, 2024
Language: Английский
Citations
6
Annual Review of Physical Chemistry, Journal Year: 2025, Volume and Issue: 76(1), P. 251 - 277
Published: April 21, 2025
No longer viewed as a passive consequence of cellular activities, membrane curvature—the physical shape the cell membrane—is now recognized an active constituent biological processes. Nanoscale topographies on extracellular matrices or substrate surfaces impart well-defined curvatures plasma membrane. This review examines events occurring at nano-bio interface, interface between and surface nanotopography, which activates intracellular signaling by recruiting curvature-sensing proteins. We encompass wide range processes including adhesion, endocytosis, glycocalyx redistribution, regulation mechanosensitive ion channels, migration, differentiation. Despite diversity processes, we call attention to critical role curvature in each process. particularly highlight studies that elucidate molecular mechanisms involving proteins with hope providing comprehensive insights into this rapidly advancing area research.
Language: Английский
Citations
0
BioEssays, Journal Year: 2025, Volume and Issue: unknown
Published: March 31, 2025
ABSTRACT Recent studies show the importance of mesoscale changes to plasma membrane (PM) topography during cell shape change. Local folding and flattening surface is mechanosensitive, changing in response both microenvironment structural elements intracellular cytoskeletal activities. These elicit local mechanical signaling events that act conjunction with molecular signal transduction pathways remodel cortex. Experimental manipulations PM curvature its sufficiency for recruiting Arp2/3 actin network induction pathways. Additionally, diverse changes—ranging from neutrophil migration early Drosophila embryo cleavage neural stem asymmetric division—show generation linked induction, which then remodels dynamic control structure. examples are reviewed detail, together known potential causes changes, downstream effects, higher‐order feedback.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 17, 2024
Cytoplasmic proteins must recruit to membranes function in processes such as endocytosis and cell division. Many of these recognize not only the chemical structure membrane lipids, but curvature surface, binding more strongly highly curved surfaces, or 'curvature sensing'. Curvature sensing by amphipathic helices is known vary with bending rigidity, changes lipid composition can simultaneously alter thickness, spontaneous curvature, leaflet symmetry, thus far preventing a systematic characterization on through either experiment simulation. Here we develop apply bilayer continuum model that tractably address this gap, quantifying how controlled each material property favor disfavor protein sensing. We evaluate both energetic structural vesicles upon helix insertion, strong agreement new vitro experiments all-atom MD simulations, respectively. Our builds previous work include monolayers via representation continuous triangular meshes. introduce coupling energy captures incompressibility established energetics tilt. In experiment, our predicts stronger distinct tail groups (POPC vs DOPC DLPC), despite having identical head-group chemistry; shows primary driving force for weaker DLPC it thinner, wedge shaped. Somewhat surprisingly, asymmetry shape between two leaflets has negligible contribution mechanics following insertion. multi-scale approach be used quantitatively efficiently predict flat surfaces driven proteins, mechanism helps target at correct time place.
Language: Английский
Citations
2
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 15, 2024
α-Synuclein (αSyn), an intrinsically disordered protein implicated in Parkinson's disease, is potentially thought to initiate aggregation through binding cellular membranes. Previous studies have suggested that anionic membrane charge necessary for this binding. However, these largely focus on unmodified αSyn, while nearly all αSyn the body N-terminally acetylated (NTA). NTA dramatically shifts narrative by diminishing αSyn's reliance Instead, we demonstrate packing defects are dominant forces driving NTA-αSyn interactions, challenging long-standing paradigm membranes essential Using fluorescence microscopy and circular dichroism spectroscopy, monitored of reconstituted surfaces with different lipid compositions. Phosphatidylcholine phosphatidylserine concentrations were varied control surface charge, phospholipid tail unsaturation methylation packing. All-atom molecular dynamics simulations bilayers supported observation defect-rich sufficient regardless charge. We further demonstrated affinity persisted reconstituted, cholesterol-containing mimicked physiological composition synaptic vesicles. Increasing mimics led more a corresponding increase Altogether, our results point mechanism regulation biological extends beyond structural properties lipids themselves.
Language: Английский
Citations
0
Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)
Published: Dec. 1, 2024
Abstract MTSS1 is a ubiquitously expressed intracellular protein known mainly for its involvement in basic cellular processes, such as the regulation of actin organization and membrane architecture. has attracted much attention role tumor suppressor, being absent or at reduced levels advanced metastasizing cancers. Occasionally, is, instead, upregulated metastasis and, some cases, even primary tumors. In addition to these well-established functions linked I-BAR- WH2-domains, involved modulating cell–cell contacts, cell differentiation, lipid metabolism, vesicle formation acts scaffolding several E3 ubiquitin ligases. classified housekeeping never mutated despite pathologic phenotypes dysregulation. Despite MTSS1’s fundamental signaling pathways, gene ablation not lethal, although it affects embryonic development. Due MTSS1´s many seemingly disparate with cases lacking mechanistic explanations, we found timely review recent data on level, well health disease, direct further studies this interesting multifunctional protein.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 28, 2024
Abstract Clathrin assembly lymphoid myeloid leukemia protein (CALM) is involved in the formation of clathrin-mediated endocytic coats by virtue binding many proteins process, including clathrin itself and AP2 cargo adaptor complex. CALM able to specifically recognize inner leaflet plasma membrane membrane’s phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P 2 ). Here, a biophysical approach, primarily using neutron X-ray scattering solid-state NMR experiments, was exploited investigate interaction with PtdIns(4,5)P -presenting model membranes. The presented experimental data reveal how folded domain partly accommodated within lipid membrane, directly interacting phosphates. Moreover, these suggest that CALM’s amphiphilic N-terminal helix buries into not only stabilising docking but also providing mechanism induce curvature.
Language: Английский
Citations
0
Soft Matter, Journal Year: 2024, Volume and Issue: 20(45), P. 8947 - 8951
Published: Jan. 1, 2024
We show the assembly and cross-linking of carboxylate-modified polystyrene nanoparticles into flexible circular, ring-shaped structures with micrometer sized diameters around base surface-adhered giant lipid vesicles.
Language: Английский
Citations
0