Leveraging CRISPR-Cas-Enhanced Isothermal Amplification Tools for Quick Identification of Pathogens Causing Livestock Diseases
Ayan Mukherjee,
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Sukhen Samanta,
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Subhasree Das
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et al.
Current Microbiology,
Journal Year:
2025,
Volume and Issue:
82(6)
Published: April 24, 2025
Language: Английский
A one-pot, one-step CRISPR-AapCas12b-based platform for sensitive and specific detection of Klebsiella pneumoniae
Juan Zhou,
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Zirong Wu,
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Lei Yu
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et al.
Sensing and Bio-Sensing Research,
Journal Year:
2025,
Volume and Issue:
48, P. 100797 - 100797
Published: April 29, 2025
Language: Английский
Detection of Potato Pathogen Clavibacter sepedonicus by CRISPR/Cas13a Analysis of NASBA Amplicons
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(22), P. 12218 - 12218
Published: Nov. 14, 2024
The
ring
rot
of
potato
caused
by
the
bacterial
pathogen
Clavibacter
sepedonicus
is
a
quarantine
disease
posing
threat
to
industry
worldwide.
sensitive
and
selective
detection
C.
high
importance
for
its
effective
control.
Here,
system
reported
determine
viable
bacteria
in
tubers,
based
on
coupling
CRISPR/Cas13a
nuclease
with
NASBA
(Nucleic
Acid
Sequence
Based
Amplification)—the
method
isothermal
amplification
RNA.
Detection
can
be
conducted
using
both
instrumental
non-instrumental
(visual
inspection
test
tubes
under
blue
light)
modes.
When
Cas13a
analyses
were
carried
out
separate
tubes,
limit
(LOD)
was
1000
copies
purified
target
16S
rRNA
per
reaction
or
about
24
colony-forming
units
(CFUs)
1
g
tuber
tissue.
testing
also
“one-pot”
format
(a
single
tube),
though
lower
sensitivity:
LOD
10,000
RNA
100
CFU
tissue
visual
overall
time
NASBA/Cas13a
analysis
did
not
exceed
2
h.
developed
has
potential
employed
as
routine
sepedonicus,
especially
on-site
testing.
Language: Английский
The current status and future prospects of CRISPR-based detection of monkeypox virus: A review
Analytica Chimica Acta,
Journal Year:
2024,
Volume and Issue:
1336, P. 343295 - 343295
Published: Oct. 1, 2024
Language: Английский
Development of a Recombinase Polymerase Amplification-Coupled CRISPR/Cas12a Platform for Rapid Detection of Antimicrobial-Resistant Genes in Carbapenem-Resistant Enterobacterales
Ji Yang,
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Heesu Kim,
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Lee-Sang Hyeon
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et al.
Biosensors,
Journal Year:
2024,
Volume and Issue:
14(11), P. 536 - 536
Published: Nov. 5, 2024
The
worldwide
spread
of
carbapenemase-producing
Language: Английский