A C2H2-type zinc finger protein TaZFP8-5B negatively regulates disease resistance DOI Creative Commons
Lin Huang, R. Xie,

Yanling Hu

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 23, 2024

Zinc finger proteins (ZFPs) are important regulators in abiotic and biotic stress tolerance plants. However, the role of ZFPs wheat responding to pathogen infection is poorly understood. In this study, we found TaZFP8-5B was down-regulated by Puccinia striiformis f. sp. tritici (Pst) infection. possesses a single C2H2-type zinc domain with plant-specific QALGGH motif, an EAR motif (LxLxL) at C-terminus. The represses trans-activation ability TaZFP8-5B. Knocking down expression TaZFP8 virus-induced gene silencing increased resistance Pst, whereas TaZFP8-5B-overexpressing reduced stripe rust rice Magnaporthe oryzae, suggesting that plays negative modulation plant immunity. Using bimolecular fluorescence complementation, split-luciferase, yeast two-hybrid assays, showed interacted calmodulin-like protein TaCML21. Knock-down TaCML21 Pst. This study characterized function its interacting Our findings provide new perspective on regulatory module made up TaCML21-TaZFP8-5B

Language: Английский

A C2H2-type zinc finger protein TaZFP8-5B negatively regulates disease resistance DOI Creative Commons
Lin Huang, R. Xie,

Yanling Hu

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 23, 2024

Zinc finger proteins (ZFPs) are important regulators in abiotic and biotic stress tolerance plants. However, the role of ZFPs wheat responding to pathogen infection is poorly understood. In this study, we found TaZFP8-5B was down-regulated by Puccinia striiformis f. sp. tritici (Pst) infection. possesses a single C2H2-type zinc domain with plant-specific QALGGH motif, an EAR motif (LxLxL) at C-terminus. The represses trans-activation ability TaZFP8-5B. Knocking down expression TaZFP8 virus-induced gene silencing increased resistance Pst, whereas TaZFP8-5B-overexpressing reduced stripe rust rice Magnaporthe oryzae, suggesting that plays negative modulation plant immunity. Using bimolecular fluorescence complementation, split-luciferase, yeast two-hybrid assays, showed interacted calmodulin-like protein TaCML21. Knock-down TaCML21 Pst. This study characterized function its interacting Our findings provide new perspective on regulatory module made up TaCML21-TaZFP8-5B

Language: Английский

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