Tissue Accumulation and Biotransformation of 6PPD-Quinone in Adult Zebrafish and Its Effects on the Intestinal Microbial Community DOI
Xiao-Liang Liao, Zhifeng Chen,

Qian-Yi Liu

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(23), P. 10275 - 10286

Published: June 3, 2024

The pronounced lethality of N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine quinone (6PPD-quinone or 6PPDQ) toward specific salmonids, while sparing other fish species, has received considerable attention. However, the underlying cause this species-specific toxicity remains unresolved. This study explored 6PPDQ toxicokinetics and intestinal microbiota composition in adult zebrafish during a 14-day exposure to environmentally realistic concentrations, followed by 7-day recovery phase. Predominant accumulation occurred brain, intestine, eyes, with lowest levels liver. Six metabolites were found undergo hydroxylation, two additionally undergoing O-sulfonation. Semiquantitative analyses revealed that predominant metabolite featured hydroxy group situated on phenyl ring adjacent quinone. was further validated assessing enzyme activity determining silico binding interactions. Notably, affinity between phase I II enzymes exceeded corresponding coho salmon 1.04–1.53 times, suggesting higher potential for detoxification tolerant species. Whole-genome sequencing significant increases genera Nocardioides Rhodococcus after 6PPDQ. Functional annotation pathway enrichment predicted these would be responsible biodegradation metabolism xenobiotics. These findings offer crucial data comprehending 6PPDQ-induced toxicity.

Language: Английский

Toxicity of substituted p-phenylenediamine antioxidants and their derived novel quinones on aquatic bacterium: Acute effects and mechanistic insights DOI
Wei Wang, Yi Chen,

Jiacheng Fang

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 469, P. 133900 - 133900

Published: Feb. 27, 2024

Language: Английский

Citations

18
AHR/cyp1b1 signaling-mediated extrinsic apoptosis contributes to 6PPDQ-induced cardiac dysfunction in zebrafish embryos DOI
Yan Jiang, Mingxuan Zhang, Jinhao Li

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 345, P. 123467 - 123467

Published: Feb. 2, 2024

Language: Английский

Citations

17
In Vitro and In Vivo Biotransformation Profiling of 6PPD-Quinone toward Their Detection in Human Urine DOI

Man Deng,

Xiaotong Ji,

Bo Peng

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(21), P. 9113 - 9124

Published: May 14, 2024

The antioxidant N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) and its oxidized quinone product 6PPD-quinone (6PPD-Q) in rubber have attracted attention due to the ecological risk that they pose. Both 6PPD 6PPD-Q been detected various environments humans cohabit. However, date, a clear understanding of biotransformation potential biomarker for exposure are lacking. To address this issue, study presents comprehensive analysis extensive across species, encompassing both vitro vivo models. We tentatively identified 17 metabolites vitro, 15 mice vivo, confirmed presence two human urine samples. Interestingly, different patterns were observed species. Through semiquantitative based on peak areas, we found almost all underwent within 24 h mice, primarily via hydroxylation subsequent glucuronidation. This suggests rapid metabolic processing mammals, underscoring importance identifying effective biomarkers exposure. Notably, monohydroxy 6PPD-Q-O-glucuronide consistently most predominant our studies, highlighting as key epidemiological research. These findings represent first data set mammalian systems, offering insights into pathways involved possible biomarkers.

Language: Английский

Citations

17
Neurotoxicity and accumulation of CPPD quinone at environmentally relevant concentrations in Caenorhabditis elegans DOI
Xin Wan, Geyu Liang, Dayong Wang

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 361, P. 142499 - 142499

Published: May 31, 2024

Language: Английский

Citations

16
Tissue Accumulation and Biotransformation of 6PPD-Quinone in Adult Zebrafish and Its Effects on the Intestinal Microbial Community DOI
Xiao-Liang Liao, Zhifeng Chen,

Qian-Yi Liu

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(23), P. 10275 - 10286

Published: June 3, 2024

The pronounced lethality of N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine quinone (6PPD-quinone or 6PPDQ) toward specific salmonids, while sparing other fish species, has received considerable attention. However, the underlying cause this species-specific toxicity remains unresolved. This study explored 6PPDQ toxicokinetics and intestinal microbiota composition in adult zebrafish during a 14-day exposure to environmentally realistic concentrations, followed by 7-day recovery phase. Predominant accumulation occurred brain, intestine, eyes, with lowest levels liver. Six metabolites were found undergo hydroxylation, two additionally undergoing O-sulfonation. Semiquantitative analyses revealed that predominant metabolite featured hydroxy group situated on phenyl ring adjacent quinone. was further validated assessing enzyme activity determining silico binding interactions. Notably, affinity between phase I II enzymes exceeded corresponding coho salmon 1.04–1.53 times, suggesting higher potential for detoxification tolerant species. Whole-genome sequencing significant increases genera Nocardioides Rhodococcus after 6PPDQ. Functional annotation pathway enrichment predicted these would be responsible biodegradation metabolism xenobiotics. These findings offer crucial data comprehending 6PPDQ-induced toxicity.

Language: Английский

Citations

16