A Combinatorial Nanobased Spray-Induced Gene Silencing Technique for Crop Protection and Improvement DOI Creative Commons

Snigdha Ghosh,

Snehanjana Patra,

Sarmistha Ray

et al.

ACS Omega, Journal Year: 2023, Volume and Issue: 8(25), P. 22345 - 22351

Published: June 14, 2023

Recent research reports have shown that plant pests and pathogens depleted the crop yield widely, which has led to an increased dependence on commercial pesticides fungicides. Increased usage of these also adverse effects environment, therefore many techniques been implemented for solving issue, some include using nanobioconjugates, RNA(i), put into use double-stranded RNAs inhibit gene expression. A more innovative eco-friendly strategy includes spray induced silencing, is being increasingly implemented. This review delves approach silencing (SIGS) in combination with used concerning various hosts their provide improved protection. Furthermore, nanotechnological advancements understood by addressing scientific gaps a rationale development updated

Language: Английский

Recent approaches and advanced wastewater treatment technologies for mitigating emerging microplastics contamination – A critical review DOI
Radhakrishnan Yedhu Krishnan,

S. Manikandan,

Ramasamy Subbaiya

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 858, P. 159681 - 159681

Published: Oct. 24, 2022

Language: Английский

Citations

166
The emerging role of nanotechnology in plant genetic engineering DOI
Henry Squire,

Sophia Tomatz,

Elizabeth Voke

et al.

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(5), P. 314 - 328

Published: Feb. 22, 2023

Language: Английский

Citations

57
Transport of Nanoparticles into Plants and Their Detection Methods DOI Creative Commons
Anca Awal Sembada, I. Wuled Lenggoro

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(2), P. 131 - 131

Published: Jan. 5, 2024

Nanoparticle transport into plants is an evolving field of research with diverse applications in agriculture and biotechnology. This article provides overview the challenges prospects associated nanoparticles plants, focusing on delivery methods detection within plant tissues. Passive assisted methods, including use roots leaves as introduction sites, are discussed, along their respective advantages limitations. The barriers encountered nanoparticle to highlighted, emphasizing need for innovative approaches (e.g., stem a new recognition site) optimize efficiency. In recent years, efforts have intensified, leading evendeeper understanding intricate mechanisms governing interaction nanomaterials tissues cells. Investigations uptake pathways translocation revealed nuanced responses different types nanoparticles. Additionally, this delves importance studying localization quantification Various techniques presented valuable tools comprehensively nanoparticle–plant interactions. reliance multiple data validation emphasized enhance reliability findings. future outlooks explored, potential alternative such stems, continued development formulations that improve adhesion penetration. By addressing these fostering multidisciplinary research, poised make significant contributions sustainable environmental management.

Language: Английский

Citations

48
Nano‐enabled stress‐smart agriculture: Can nanotechnology deliver drought and salinity‐smart crops? DOI Creative Commons
Ali Raza, Sidra Charagh, Hajar Salehi

et al.

Journal of Sustainable Agriculture and Environment, Journal Year: 2023, Volume and Issue: 2(3), P. 189 - 214

Published: July 18, 2023

Abstract Salinity and drought stress substantially decrease crop yield superiority, directly threatening the food supply needed to meet rising needs of growing total population. Nanotechnology is a step towards improving agricultural output tolerance by efficacy inputs in agriculture via targeted delivery, controlled release, enhanced solubility adhesion while also reducing significant damage. The direct application nanoparticles (NPs)/nanomaterials can boost performance effectiveness physio‐biochemical molecular mechanisms plants under conditions, leading advanced tolerance. Therefore, we presented effects plant responses explored potential nanomaterials for systems, discussed advantages applying NPs at various developmental stages alleviate negative salinity stress. Moreover, feature recent innovations state‐of‐the‐art nanobiotechnology, specifically NP‐mediated genome editing CRISPR/Cas system, develop stress‐smart crops. However, further investigations are unravel role nanobiotechnology addressing climate change challenges modern systems. We propose that combining speed breeding techniques could enable designing climate‐smart cultivars (particularly bred or genetically modified varieties) security world

Language: Английский

Citations

46
Impact of Heavy Metal Pollution in the Environment on the Metabolic Profile of Medicinal Plants and Their Therapeutic Potential DOI Creative Commons

Dana-Mihaela Asiminicesei,

Daniela Ionela Ferţu, Maria Gavrilescu

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(6), P. 913 - 913

Published: March 21, 2024

The paper provides a comprehensive examination of heavy metal stress on medicinal plants, focusing its impact antioxidant capacity and biosynthetic pathways critical to their therapeutic potential. It explores the complex relationship between metals physiological biochemical responses highlighting how disrupts pathways, altering concentrations secondary metabolites. This disruption may compromise overall quality efficacy requiring holistic understanding cumulative impacts. Furthermore, study discusses potential targeted genetic editing enhance plant resilience against by manipulating genes associated with defenses. approach represents promising frontier in safeguarding plants metal-contaminated environments. Additionally, research investigates role phytohormone signaling adaptive mechanisms stress, revealing influence responses, thereby adding complexity adaptation. underscores importance innovative technologies global cooperation protecting plants’ highlights need for mitigation strategies address contamination effectively.

Language: Английский

Citations

35
Advances in Delivery Mechanisms of CRISPR Gene-Editing Reagents in Plants DOI Creative Commons
Larissa C. Laforest, Satya Swathi Nadakuduti

Frontiers in Genome Editing, Journal Year: 2022, Volume and Issue: 4

Published: Jan. 24, 2022

Gene-editing by CRISPR/Cas systems has revolutionized plant biology serving as a functional genomics tool. It tremendously advanced breeding and crop improvement accelerating the development of improved cultivars, creating genetic variability, aiding in domestication wild orphan crops. is rapidly evolving field. Several advancements include different Cas effectors with increased target range, efficacy, enhanced capacity for precise DNA modifications base editing prime editing. The existing toolbox various CRISPR reagents facilitate gene knockouts, targeted insertions, substitutions, multiplexing. However, major challenge genome-editing remains efficient delivery these into cells. Plants have larger more complex genome structures compared to other living due common occurrence polyploidy re-arrangements. Further, rigid cell walls surrounding cells deter entry any foreign biomolecules. Unfortunately, transformation deliver gene-editing been established only limited number species. Recently, there significant progress plants. This review focuses on exploring mechanisms categorized Agrobacterium -mediated breakthroughs, particle bombardment-based biomolecules recent improvements, protoplasts, versatile system regeneration ultimate goal establish highly genotype-independent reagent multiple targets simultaneously achieve DNA-free gene-edited plants at scale.

Language: Английский

Citations

54
CRISPR/Cas systems for genomic Editing, biochemical Sensing, Bioanalysis, and diagnostics DOI
Mirza Muhammad Faran Ashraf Baig, Sek Ying Chair, Wai Tong Chien

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112638 - 112638

Published: Jan. 1, 2025

Language: Английский

Citations

1
Molecular Farming for Immunization: Current Advances and Future Prospects in Plant-Produced Vaccines DOI Creative Commons
Dang-Khoa Vo, Kieu The Loan Trinh

Vaccines, Journal Year: 2025, Volume and Issue: 13(2), P. 191 - 191

Published: Feb. 15, 2025

Using plants as bioreactors, molecular farming has emerged a versatile and sustainable platform for producing recombinant vaccines, therapeutic proteins, industrial enzymes, nutraceuticals. This innovative approach leverages the unique advantages of plants, including scalability, cost-effectiveness, reduced risk contamination with human pathogens. Recent advancements in gene editing, transient expression systems, nanoparticle-based delivery technologies have significantly enhanced efficiency versatility plant-based systems. Particularly vaccine development, demonstrated its potential notable successes such Medicago's Covifenz COVID-19, illustrating capacity platforms to address global health emergencies rapidly. Furthermore, edible vaccines opened new avenues mainly settings low resources where cold chain used conventional logistics is challenge. However, optimization protein yield stability, complexity purification processes, regulatory hurdles are some challenges that still remain. review discusses current status development using operational mechanisms plant platforms, major applications prevention infectious diseases, developments, nanoparticle-mediated cancer vaccines. The discussion will also touch on ethical considerations, framework, future trends respect transformative plant-derived ensuring greater accessibility cost-effectiveness vaccination. field holds great promise disease area and, indeed, personalized medicine biopharmaceuticals near future.

Language: Английский

Citations

1
Integrating RNA Interference and Nanotechnology: A Transformative Approach in Plant Protection DOI Creative Commons
Mohammad Shafiqul Islam, Md Robel Ahmed, Muhammad Noman

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 977 - 977

Published: March 20, 2025

RNA interference (RNAi) has emerged as a potent mechanism for combating pathogenic fungi and oomycetes over the past decades. It offers promising gene-silencing approach by targeting crucial genes involved in diseases caused economically scientifically significant fungal pathogens, such Botrytis cinerea Fusarium species. Simultaneously, nano-agro-products have gained attention alternatives to traditional fungicides plant protection strategies. However, instability of naked molecules outside cellular environment presents challenge, they degrade rapidly, limiting their efficacy prolonged disease control. Concerns regarding toxicity protective nanoparticles non-target organisms also arisen. Integrating RNAi with nano-agro-products, particularly nanocarriers, form RNA-nano complexes demonstrated potential, providing enhanced stability, reduced toxicity, extended This review explores mechanisms complexes-mediated protection, addressing stability nano-toxicity issues while examining prospects complex research pathogen management.

Language: Английский

Citations

1
A review on the fate and transport behavior of engineered nanoparticles: possibility of becoming an emerging contaminant in the groundwater DOI

K. Dibyanshu,

T. Chhaya,

Trishikhi Raychoudhury

et al.

International Journal of Environmental Science and Technology, Journal Year: 2022, Volume and Issue: 20(4), P. 4649 - 4672

Published: Jan. 17, 2022

Language: Английский

Citations

31