In Vitro Evaluation of Silver-Zinc Oxide-Eugenol Nanocomposite for Enhanced Antimicrobial and Wound Healing Applications in Diabetic Conditions DOI Creative Commons
Shunmugiah Karutha Pandian

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

Abstract Diabetic wounds with chronic infections present a significant challenge, exacerbated by the growing issue of antimicrobial resistance, which often leads to delayed healing and increased morbidity. This study introduces novel silver-zinc oxide-eugenol (Ag+ZnO+EU) nanocomposite, specifically designed enhance activity promote wound healing. The nanocomposite was thoroughly characterized using advanced analytical techniques, confirming its nanoscale structure, stability chemical composition. Ag+ZnO+EU demonstrated potent efficacy against range associated pathogens, including standard clinical isolates Staphylococcus aureus, Pseudomonas aeruginosaand Candida albicans. Minimum inhibitory concentrations for were significantly lower than those individual components, highlighting synergistic effect nanocomposite. Time-kill assays revealed rapid microbial eradication, achieving complete sterility within 240-min. Importantly, effectively eliminated persister-like cells, are typically resistant conventional treatments, suggesting potential solution persistent infections. In vitro scratch human keratinocyte cells that accelerated closure, near-complete observed 24 hours, indicating enhanced cell migration tissue regeneration. Additionally, showed antidiabetic effects increasing glucose uptake up 97.21% in an in assay 2- [N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG), fluorescent analog, applications beyond These findings highlight as promising candidate addressing both resistance impaired diabetic contexts.

Language: Английский

Micro-interfacial behavior of antibiotic-resistant bacteria and antibiotic resistance genes in the soil environment: A review DOI Creative Commons

Jibao Deng,

W. Zhang, Lingyu Zhang

et al.

Environment International, Journal Year: 2024, Volume and Issue: 191, P. 108972 - 108972

Published: Aug. 22, 2024

Overutilization and misuse of antibiotics in recent decades markedly intensified the rapid proliferation diffusion antibiotic resistance genes (ARGs) within environment, thereby elevating ARGs to status a global public health crisis. Recognizing that soil acts as critical reservoir for ARGs, environmental researchers have made great progress exploring sources, distribution, spread soil. However, microscopic state micro-interfacial behavior remains inadequately understood. In this study, we reviewed behaviors antibiotic-resistant bacteria (ARB) porous media, predominantly including migration-deposition, adsorption, biofilm formation. Meanwhile, proliferation, degradation were identified primary soil, with component serving significant determinant. Our work contributes further comprehension microstates processes ARB environments offers theoretical foundation managing mitigating risks associated ARG contamination.

Language: Английский

Citations

10
In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions DOI Creative Commons

Hari Prasath Nagaiah,

Malik Basha Samsudeen,

Akshaya Rani Augustus

et al.

Discover Nano, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 23, 2025

Diabetic wounds with chronic infections present a significant challenge, exacerbated by the growing issue of antimicrobial resistance, which often leads to delayed healing and increased morbidity. This study introduces novel silver-zinc oxide-eugenol (Ag+ZnO+EU) nanocomposite, specifically designed enhance activity promote wound healing. The nanocomposite was thoroughly characterized using advanced analytical techniques, confirming its nanoscale structure, stability chemical composition. Ag+ZnO+EU demonstrated potent efficacy against range associated pathogens, including standard clinical isolates Staphylococcus aureus, Pseudomonas aeruginosa Candida albicans. Minimum inhibitory concentrations for were significantly lower than those individual components, highlighting synergistic effect nanocomposite. Time-kill assays revealed rapid microbial eradication, achieving complete sterility within 240-min. Importantly, effectively eliminated persister-like cells, are typically resistant conventional treatments, suggesting potential solution persistent infections. In vitro scratch human keratinocyte cells that accelerated closure, near-complete observed 24-h, indicating enhanced cell migration tissue regeneration. Additionally, showed antidiabetic effects increasing glucose uptake up 97.21% in an assay 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose, fluorescent analog, applications beyond These findings highlight as promising candidate addressing both resistance impaired diabetic contexts.

Language: Английский

Citations

2
Preparation of bacterial cellulose-based antimicrobial materials and their applications in wound dressing: A review DOI Creative Commons
Reema Gabrani,

Jiahuan Xu,

Beijia Zhang

et al.

Materials & Design, Journal Year: 2025, Volume and Issue: unknown, P. 113820 - 113820

Published: March 1, 2025

Language: Английский

Citations

1
Long-Term Outcomes of a Health Information System-Based Feedback Intervention Study of Antimicrobial Prescriptions in Primary Care Institutions: Follow-Up of a Randomized Cross-Over Controlled Trial DOI Creative Commons
Yunzi Yan, Jun‐Li Yang, Yun Lu

et al.

Infection and Drug Resistance, Journal Year: 2025, Volume and Issue: Volume 18, P. 61 - 76

Published: Jan. 1, 2025

To evaluate the long-term impacts of feedback intervention on controlling inappropriate use antimicrobial prescriptions in primary care institutions China, as a continuation previous trial. After ended, we conducted 12-month follow-up study. The prescription data were collected from baseline until end period. generalized estimation equation was employed to analyze differences among four representative time points: at point, 3 months, 6 and 18 months. time-intervention interaction utilized changing trends group A B. Our outcome variable is monthly rate (IAPR). adjusting for covariates, IAPRs decreased by 1.00% average point 5.00% months -0.92% 0.39% During corresponding periods B, decline 2.33%, 3.67%, -0.42%, 0.72%, respectively. As rates (APRs), 1.33%, 0.17% during three periods: Accordingly 1.00%, 0.08%, generated limited impacts. Although APRs consistently remained below both experienced rebound within certain range following stop two groups. It reasonable think that desired effects will be difficult maintain without sustained implementation intervention.

Language: Английский

Citations

0
Multifunctional Waterproof Hydrogels Based on Copper Sulfide Nanoparticles for Burn Wound Healing DOI Open Access
Jie Wu, Lei Dai, Limei Zhao

et al.

Journal of Polymer Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

ABSTRACT Burn wounds care and healing are very difficult in clinic. Herein, a multifunctional waterproof hydrogel was fabricated with photothermal antimicrobial NO anti‐inflammatory properties. In the as‐prepared hydrogel, polyethyleneimine (PEI) thioctic acid (TA) were cross‐linked by electrostatic interactions hydrogen bonding while aggregation of TA could be favor to formation hydrophobic caves. Benefit from caves, CuS BNN6 (N, N′‐dibutyl‐N, N′‐dinitroso‐1,4‐phenylenediamine) incorporated PEI/TA‐based air permeability also increased. Interestingly, mild increased temperature conversion not only endowed ability but promoted release BNN6. The PEI/TA/CuS/BNN6 good biocompatibility, self‐healing water resistance suitable for promoting burn healing. It provides new platform designing novel wound dressing wounds.

Language: Английский

Citations

0
Proline-Modified (RW)n Peptides: Enhancing the Antimicrobial Efficacy and Selectivity against Multidrug-Resistant Pathogens DOI Creative Commons
Anderson Sunda‐Meya,

Nsoki Phambu

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

The growing threat of multidrug-resistant (MDR) bacteria necessitates the development novel antimicrobial agents. This study investigates potential proline-modified (RW)n peptides as a platform for combating MDR pathogens with minimal toxicity. We synthesized and evaluated (n = 4, 6, 8) without central proline residues against five clinically relevant bacterial strains, including ESKAPE pathogens. Antimicrobial activity, cytotoxicity, synergistic effects conventional antibiotics were assessed. Proline incorporation significantly enhanced peptide selectivity broadened spectrum particularly Gram-negative bacteria. RW6P RW8P demonstrated exceptional efficacy (MICs ≤ 0.25 μg/mL) methicillin-resistant Staphylococcus aureus Escherichia coli toxicity to human cells. Notably, restored ampicillin susceptibility in Pseudomonas aeruginosa (MIC < dose-dependent Exceptionally, RW6-2P all Gram-positive bacteria, except Klebsiella pneumoniae (Kp), Furthermore, several isolates rendered susceptible vancomycin when combined these peptides, addressing key limitation glycopeptide antibiotics. (Kp) was RW4P RW6-2P. RW6-2P, antibiotics, have shown selectivity. presents promising candidates developing broad-spectrum antimicrobials revitalize existing

Language: Английский

Citations

0
Harnessing the CRISPR/Cas9 in filamentous fungi for the production of secondary metabolites DOI

Md Dilshad Karim,

Md Abuhena,

Lutfur Rahman

et al.

Systems Microbiology and Biomanufacturing, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Language: Английский

Citations

0
An efficient synthesis of novel quinolone-thiazolo[3,2-a] pyrimidine hybrid via a facile one-pot, a three-component reaction catalyzed by L-proline as an organo-catalyst DOI

Homeyra Masoudipour,

Abbas Ali Esmaeili

Journal of Sulfur Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 19

Published: March 25, 2025

Language: Английский

Citations

0
Fighting Antibiotic Resistance: Insights Into Human Barriers and New Opportunities DOI Creative Commons

Aubin Pitiot,

Camille Rolin, Carole Seguin‐Devaux

et al.

BioEssays, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

The public health issue of bacterial multi-resistance to antibiotics has gained awareness among the public, researchers, and pharmaceutical sector. Nevertheless, spread antimicrobial resistance been considerably aggravated by human activities, climate change, subsequent increased release antibiotics, drug-resistant bacteria, antibiotic genes in environment. extensive use for medical veterinary purposes not only induced increasing but also other problems, including negative effects on patient's microbiome. Preventive strategies, new treatment modalities, surveillance are progressively set up. A comprehensive approach is, however, lacking urgently tackling this adverse situation. To address challenge, we discussed here main causes driving pollution environment factors favorable emergence drug resistance. We next propose some key priorities research, prevention, surveillance, education supervise an effective clinical sustainable response.

Language: Английский

Citations

0
Identification of hydroxyphenyl cyanovinyl thiazoles as new structural scaffold of potential antibacterial agents DOI

Lu Xing,

Shaolin Zhang, Cheng‐He Zhou

et al.

Bioorganic & Medicinal Chemistry Letters, Journal Year: 2025, Volume and Issue: unknown, P. 130258 - 130258

Published: April 1, 2025

Language: Английский

Citations

0