Frontiers in Bioengineering and Biotechnology,
Journal Year:
2023,
Volume and Issue:
10
Published: Jan. 4, 2023
Coacervates
are
droplets
formed
by
liquid‒liquid
phase
separation.
An
increasing
number
of
studies
have
reported
that
coacervates
play
an
important
role
in
living
cells,
such
as
the
generation
membraneless
organelles,
and
peptides
contribute
to
condensate
droplet
formation.
Peptides
with
versatile
functional
groups
special
secondary
structures,
including
α-helices,
β-sheets
intrinsically
disordered
regions,
provide
novel
insights
into
coacervation,
biomimetic
protocells,
neurodegenerative
diseases,
modulations
signal
transmission,
drug
delivery
systems.
In
this
review,
we
introduce
different
types
peptide-based
principles
their
interactions.
Additionally,
summarize
thermodynamic
kinetic
mechanisms
associated
factors,
salt,
pH,
temperature,
affecting
separation
process.
We
illustrate
recent
on
modulating
functions
applied
biological
diseases.
Finally,
propose
promising
broad
applications
describe
challenges
future.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
36(24)
Published: March 27, 2024
Abstract
Broad
size
distributions
and
poor
long‐term
colloidal
stability
of
microRNA‐carrying
nanoparticles,
especially
those
formed
by
polyelectrolyte
complexation,
represent
major
hurdles
in
realizing
their
clinical
translation.
Herein,
peptide
design
is
used
alongside
optimized
flash
nanocomplexation
(FNC)
to
produce
uniform
peptide‐based
miRNA
particles
exceptional
that
display
anticancer
activity
against
mesothelioma
vitro
vivo.
Modulating
the
content
lysine‐based
charge
from
small
intrinsically
disordered
peptides
complex
proves
essential
achieving
stable
colloids.
FNC
facilitates
kinetic
isolation
mechanistic
steps
involved
particle
formation
allow
preparation
discrete
a
highly
reproducible,
scalable,
continuous
manner,
facilitating
pre‐clinical
studies.
To
best
authors
knowledge,
this
work
represents
first
example
employing
prepare
complexes
peptide.
Encapsulation
these
into
an
injectable
hydrogel
matrix
allows
for
localized
vivo
delivery
syringe.
A
one‐time
injection
gel
containing
composed
miRNA‐215‐5p
PKM1
limits
tumor
progression
xenograft
model
mesothelioma.
ACS Applied Materials & Interfaces,
Journal Year:
2022,
Volume and Issue:
14(38), P. 43825 - 43832
Published: Sept. 14, 2022
The
movements
of
soft
living
tissues,
such
as
muscle,
have
sparked
a
strong
interest
in
the
design
hydrogel
actuators;
however,
so
far,
typical
manmade
examples
still
lag
behind
their
biological
counterparts,
which
usually
function
under
nonequilibrium
conditions
through
consumption
high-energy
biomolecules
and
show
highly
autonomous
behaviors.
Here,
we
report
on
self-resettable
actuators
that
are
powered
by
chemical
fuel
can
spontaneously
return
to
original
states
over
time
once
fuels
depleted.
Self-resettable
actuation
originates
from
fuel-mediated
transient
change
hydrophilicity
networks.
extent
duration
be
programmed
levels,
process
is
recyclable
refueling.
Furthermore,
various
proof-of-concept
robots
created,
resembling
soft-bodied
creatures
nature.
This
work
may
serve
starting
point
for
development
lifelike
with
Chemical Science,
Journal Year:
2022,
Volume and Issue:
13(16), P. 4533 - 4544
Published: Jan. 1, 2022
Fuel-driven
macromolecular
coacervation
is
an
entry
into
the
transient
formation
of
highly
charged,
responsive
material
phases.
In
this
work,
we
used
a
chemical
reaction
network
(CRN)
to
drive
species
readily
produced
using
radical
polymerisation
methods.
The
CRN
enables
quaternization
tertiary
amine
substrates,
driven
by
conversion
electron
deficient
allyl
acetates
and
thiol
or
nucleophiles.
By
incorporating
functionality
block
copolymers,
demonstrate
triggered
complex
coacervate
core
micelle
(C3M)
assembly
disassembly.
contrast
most
dynamic
systems,
operates
at
constant
physiological
pH
without
need
for
biomolecules.
varying
acetate
fuel,
deactivating
nucleophile
reagent
ratios,
achieved
both
sequential
signal-induced
C3M
(dis)assembly,
as
well
non-equilibrium
(dis)assembly.
We
expect
that
timed
signal-responsive
control
over
phase
will
find
application
in
nucleic
acid
delivery,
nano
reactors
protocell
research.
Angewandte Chemie International Edition,
Journal Year:
2022,
Volume and Issue:
61(32)
Published: June 3, 2022
Abstract
Active
droplets
are
a
great
model
for
membraneless
organelles.
However,
the
analysis
of
these
systems
remains
challenging
and
is
often
limited
due
to
short
timescales
their
kinetics.
We
used
droplet‐based
microfluidics
encapsulate
fuel‐driven
cycle
that
drives
phase
separation
into
coacervate‐based
overcome
this
challenge.
This
approach
enables
every
droplet
in
reaction
container
throughout
its
lifetime.
discovered
fuel
concentration
dictates
formation
properties.
observed
grow
through
fusion,
decay
simultaneously
independent
volume,
shrinkage
rate
scales
with
initial
volume.
method
helps
further
understand
regulation
organelles,
we
believe
individual
future
selection‐
or
evolution‐based
studies.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2023,
Volume and Issue:
10
Published: Jan. 4, 2023
Coacervates
are
droplets
formed
by
liquid‒liquid
phase
separation.
An
increasing
number
of
studies
have
reported
that
coacervates
play
an
important
role
in
living
cells,
such
as
the
generation
membraneless
organelles,
and
peptides
contribute
to
condensate
droplet
formation.
Peptides
with
versatile
functional
groups
special
secondary
structures,
including
α-helices,
β-sheets
intrinsically
disordered
regions,
provide
novel
insights
into
coacervation,
biomimetic
protocells,
neurodegenerative
diseases,
modulations
signal
transmission,
drug
delivery
systems.
In
this
review,
we
introduce
different
types
peptide-based
principles
their
interactions.
Additionally,
summarize
thermodynamic
kinetic
mechanisms
associated
factors,
salt,
pH,
temperature,
affecting
separation
process.
We
illustrate
recent
on
modulating
functions
applied
biological
diseases.
Finally,
propose
promising
broad
applications
describe
challenges
future.
