Molecular dissection of tendon development and healing: insights into tenogenic phenotypes and functions

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108353 - 108353

Published: Feb. 1, 2025

Language: Английский

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Viscoelastic Mechanics of Living Cells

Physics of Life Reviews, Journal Year: 2025, Volume and Issue: 53, P. 91 - 116

Published: March 1, 2025

Language: Английский

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Biotensegrity: Concept, Principles and Applications

Courses and lectures, Journal Year: 2025, Volume and Issue: unknown, P. 151 - 174

Published: Jan. 1, 2025

Language: Английский

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Genetically encoded tension heterogeneity sculpts cardiac trabeculation

Science Advances, Journal Year: 2025, Volume and Issue: 11(10)

Published: March 7, 2025

The myocardial wall arises from a single layer of cardiomyocytes, some delaminate to create trabeculae while others remain in the compact layer. However, mechanisms governing cardiomyocyte fate decisions unclear. Using single-cell RNA sequencing, genetically encoded biosensors, and toto live imaging, we observe intrinsic variations erbb2 expression its association with trabecular fate. Specifically, promotes PI3K activity recruits Arp2/3 complex, inducing polarized accumulation actomyosin network drive cell delamination. Subsequently, lineage-committed nascent trigger Notch neighboring cardiomyocytes suppress reduce tension, thereby confining them Overall, this genetic cellular interplay governs determination orchestrate pattern formation.

Language: Английский

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Uniform and Controllable Surface Nano-structure on Polyetheretherketone Implants Can Regulate Mechanical Property to Enhance Soft Tissue Integration Through Piezo1/TGF-β1 Signaling Axis

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101645 - 101645

Published: March 8, 2025

Polyetheretherketone (PEEK) has potential to repair the orbital floor bone defects following craniofacial trauma and surgery. However, inertness of material impedes soft tissue integration implants, leading complications such as implant migration infection. Surface patterning modification on PEEK can promote surface hydrophily enhance better integration, but it is difficult obtain uniform controllable nano-structure. In this study, hot-pressing technology was used produce nanopores with a diameter 200, 500, 800 nm. Depending craft, surficial mechanical properties be regulated assessed by finite element analysis. Furthermore, 500 nm interface proliferation, migration, fibrosis fibroblasts achieved optimal effects in animal implantation experiments. To explore mechanism biological responses, transcriptomics molecular biology experiments revealed that Piezo1/TGF-β1 axis played critical role response cells stimulation PEEK. Our study established novel for constructing nanostructures PEEK, thereby promoting tissues implants improving anchoring effect.

Language: Английский

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Mechanical Behavior and Indentation-Induced Injury of Soft Microtissues with Different Densification Levels

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Language: Английский

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Gas Bubbles from Biodegradable Magnesium Implants Convey Mechanical Cues and Promote Immune Cell Stimulation

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 11, 2025

Abstract In ever‐increasing numbers, patients are treated with biodegradable magnesium implants. While gas bubbles frequently arise in soft tissue overlying implants, their biological implications remain uncertain. This study investigates how bubble accumulation and evolution across various lengths time scales influence adjacent cell behavior rats. Bubbles accumulate tissues around during initial postimplantation days, then fully resorb. Alterations geometry coincide of cells, many macrophage phenotypes, increased expression the mechanosensitive ion‐channel Piezo1. Using spatially resolved transcriptomics, strong proinflammatory pathway activation is revealed near marked proliferative marker secreted phosphoprotein 1 ( Spp1 ). Spatial transcriptomics also reveals enrichment cytoskeletal rearrangement genes, demonstrating that cells respond to mechanical cues from bubbles. Notably, both bubble–implant distance strongly cellular response. Over time, as located farther implant, regenerative processes decline, inflammation predominates. These findings suggest implant degradation create an intricate local response influencing healing through inflammatory pathways. underscores need for implants controlled release meticulous monitoring patients.

Language: Английский

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Transcriptome sequencing-based analysis of primary vein development in Betula pendula ‘Dalecarlica’

Gene, Journal Year: 2024, Volume and Issue: unknown, P. 148948 - 148948

Published: Sept. 1, 2024

Language: Английский

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Multiscale mechanics drive functional maturation of the vertebrate heart

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Abstract How simple tissue primordia sculpt complex functional organs, robustly and reproducibly, remains elusive. As the zebrafish embryo grows into a larva, to improve its heart function, embryonic myocardial wall transforms an intricate 3D architecture, composed of outer compact layer enveloping inner multicellular trabecular ridges. these layers acquire their characteristic form suited for function open question. Here, we find that multiscale mechanochemical coupling emergent tissue-scale morphological transition steer maturation developing heart. Single-celled seeds recruit cells mature clonally heterogenous ridges, thereby amplifying cardiac contractile forces. In response, remaining are stretched, which impedes further recruitment, constraining ridge density. Concomitantly, Notch-dependent actomyosin dampening triggers sharp in area, activating rapid organ growth expands blood filling capacity. Thus, self-organizing interactions optimize size efficiency support life.

Language: Английский

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Lattice Boltzmann modeling of individual and collective cell dynamics in the presence of fluid flows

Physics of Fluids, Journal Year: 2024, Volume and Issue: 36(10)

Published: Oct. 1, 2024

Dynamics of individual and collective cells with fluid flow is an important attractive topic in both the areas science biomedical engineering. The kinetic theory-based lattice Boltzmann model presented to describe multicellular dynamics coupled flows. In model, Bhatnagar–Gross–Krook (BGK) equation extended cellular dynamics. Through Chapman–Enskog analysis, BGK–Boltzmann for can be recovered phase field equation, capturing intricate processes cell motion deformation. Simultaneously, governing Navier–Stokes equations, enabling accurate representation characteristics. Both equations are directly discretized into scheme, providing efficient robust computational approach. After validation verification, dynamical behavior spheroids shear flows were sequentially investigated. Apart from observation elongation rotation spheroids, quantitative analyses conducted across several key factors. results show that physical properties significantly impact rheology this effect related intercellular interactions. Furthermore, morphogenesis aggregate under influence inflow was investigated, revealing remarkable deformation along formation a cavity. This study demonstrates potentiality proposed theory based method simulating microscopic biofluidic systems, novel numerical tool explore interactions between their hydrodynamical environments.

Language: Английский

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