Ultra-fast light-field microscopy with event detection DOI Creative Commons
Liheng Bian, Xuyang Chang, Hanwen Xu

et al.

Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)

Published: Nov. 7, 2024

The event detection technique has been introduced to light-field microscopy, boosting its imaging speed in orders of magnitude with simultaneous axial resolution enhancement scattering medium.

Language: Английский

Dark-based optical sectioning assists background removal in fluorescence microscopy DOI
Ruijie Cao, Yaning Li, Yao Zhou

et al.

Nature Methods, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

Language: Английский

Citations

3
Epigenetic gene regulation in plants and its potential applications in crop improvement DOI
Heng Zhang,

Jian-Kang Zhu

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: 26(1), P. 51 - 67

Published: Aug. 27, 2024

Language: Английский

Citations

11
Long-term mesoscale imaging of 3D intercellular dynamics across a mammalian organ DOI Creative Commons
Yuanlong Zhang, Mingrui Wang,

Qiyu Zhu

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

11
Molecular probes for in vivo optical imaging of immune cells DOI
Jing Liu, Penghui Cheng, Cheng Xu

et al.

Nature Biomedical Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Language: Английский

Citations

1
Physics-driven self-supervised learning for fast high-resolution robust 3D reconstruction of light-field microscopy DOI Creative Commons
Zhi Lü, Manchang Jin, Daiwen Chen

et al.

Nature Methods, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

Language: Английский

Citations

1
Migrasomes: Biogenesis, physiological roles, and therapeutic potentials DOI Creative Commons
Haifeng Jiao,

Li Yu

The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 223(11)

Published: Oct. 14, 2024

Migrasomes, vesicular structures discovered in migrating cells, arise from the junctions or tips of retraction fibers, and gradually grow to microscale vesicles. Migrasomes have garnered attention for their role intercellular communication potential therapeutic implications. This review presents an overview recent advances migrasome biology, covering mechanisms biogenesis, essential physiological roles, association with various diseases, alongside applications. Furthermore, we share our perspectives on future directions study migrasomes highlight challenges that remain this developing area research.

Language: Английский

Citations

5
Label-free 3D optical angiography via time-frequency domain analysis of focal modulated dynamic blood flow DOI
Shangpan Yang,

Yingsi Yu,

Huadeng Chen

et al.

Applied Physics Letters, Journal Year: 2025, Volume and Issue: 126(5)

Published: Feb. 3, 2025

Accurate three-dimensional (3D) blood flow imaging with high spatiotemporal resolution and significant detection depth is essential for studying vascular structure-related diseases. In this Letter, we introduce a label-free 3D optical angiography technique via time-frequency domain analysis (TFDA) of focal modulated dynamic flow. First, low-magnification telecentric lens used sparse axial sampling within large depth-of-field range to obtain coarse estimate depth. Then, based on the frequency-depth characteristics signals, TFDA-based focusing evaluation function established in combination Lambert–Beer's law, achieving mean absolute percentage error 2.06%. Finally, validation 3-day-old chicken embryo demonstrated lateral spatial 2.95 μm time 11.5 s 4.95 × 0.7 mm3 sample. Our method provides effective localization by assessing modulation intensity relative plane position, offering promising support disease research.

Language: Английский

Citations

0
Plasma cfDNA VILL gene methylation as a diagnostic marker for nasopharyngeal carcinoma DOI Creative Commons
Xiaoyan Fu, Ziying Zhou,

Teng-Yue Yang

et al.

Clinical Epigenetics, Journal Year: 2025, Volume and Issue: 17(1)

Published: Feb. 27, 2025

Currently, the non-invasive diagnostic methods for nasopharyngeal carcinoma (NPC) continue to grapple with challenge of low sensitivity. The hypermethylation tumor suppressor genes is an established early event in NPC pathogenesis. Consequently, we conducted whole-genome methylation sequencing on plasma cell-free DNA (cfDNA) from six cases and four healthy controls, integrating Illumina Human Methylation 450 K microarray data GEO database comprising samples non-cancerous tissue (NP). As result, screened only one CpG island associated cell type-specific regulation within candidate gene VILL (Vilin Like), which exhibits specific patterns NPC. We validated our findings using 25 pairs NP GEO, alongside 9,736 pan-cancer tissues TCGA 656 human leukocyte sourced through analysis. Based this, designed a methylation-specific qPCR (qMSP) system gene, then tested it 192 primary 154 NC samples. new qMSP when compared EBV revealed sensitivity 80.2% vs.81.3% (78.8% vs.54.5% early-stage NPC), specificity 100% vs. 93.5%. Notably, employing combined methodology further enhanced 94.8%, including rate 90.9% diagnosis. Therefore, assessment detection presents promising avenue diagnosis NPC, particularly beneficial detection.

Language: Английский

Citations

0
Comments on high-speed mesoscale light-field microscopy DOI
Euiheon Chung,

Youngseung Yoo,

C. Hwang

et al.

Advanced imaging., Journal Year: 2025, Volume and Issue: 2(2), P. 023001 - 023001

Published: Jan. 1, 2025

Language: Английский

Citations

0
Empowering Biomedical Research with Foundation Models in Computational Microscopy: A Systematic Review DOI Creative Commons

Di Ding,

Tianliang Yao, Feng Gao

et al.

Advanced Intelligent Systems, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

The integration of foundation models in artificial intelligence (AI) is transforming computational microscopy, driving significant advancements biomedical research. Trained on extensive and diverse datasets, these overcome critical limitations traditional such as low resolution, slow processing speeds, difficulties analyzing complex, high‐dimensional biological data. Foundation enable enhanced image accelerated data processing, real‐time analysis dynamic processes. Despite advancements, challenges persist, including concerns related to quality, model generalizability across varied contexts, the interpretability AI‐generated insights. This review explores application emphasizing their theoretical foundations practical implications disciplines. Key obstacles are identified, requirement for large‐scale, high‐quality annotated datasets need adaptation specific clinical preclinical settings. highlights transformative potential advancing precision medicine, improving disease diagnostics, enabling innovative therapeutic strategies, ultimately reshaping landscape

Language: Английский

Citations

0