NADPH Oxidases‐Inspired Reactive Oxygen Biocatalysts with Electron‐Rich Pt Sites to Potently Amplify Immune Checkpoint Blockade Therapy DOI Open Access

Chuyi Han,

Sutong Xiao, Zhenyu Xing

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 14, 2024

Abstract Clinical immune checkpoint blockade (ICB)‐based immunotherapy of malignant tumors only elicits durable responses in a minority patients, primarily due to the highly immunosuppressive tumor microenvironment. Although inducing immunogenic cell death (ICD) through reactive oxygen biocatalyst represents an attractive therapeutic strategy amplify ICB, currently reported biocatalysts encounter insurmountable challenges achieving high ROS‐generating activity induce potent ICD. Here, inspired by natural catalytic characteristics NADPH oxidases, design efficient, robust, and electron‐rich Pt‐based redox centers on non‐stoichiometric W 18 O 49 substrates (Pt─WO x ) serve as bioinspired potently activate ICD, which eventually enhance cancer amplifies ICB‐based is reported. These studies demonstrate that Pt─WO exhibits rapid electron transfer capability can promote formation low oxophilic Pt for superior biocatalysis, enables ‐based inducers trigger endoplasmic reticulum stress directly stimulate amplifying anti‐PD‐L1‐based ICB therapy. This provides straightforward engineer also opens up new avenue create efficient ICD primary/metastatic treatments.

Language: Английский

A pH‐Sensitive Glucose Oxidase and Hemin Coordination Micelle for Multi‐Enzyme Cascade and Amplified Cancer Chemodynamic Therapy DOI

Zhen Jiang,

Jiexin Li,

Gengqi Liu

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 4, 2024

Abstract Chemodynamic therapy (CDT) is an emerging therapeutic paradigm for cancer treatment that utilizes reactive oxygen species (ROS) to induce apoptosis of cells but few biomaterials have been developed differentiate the and normal achieve precise targeted CDT. Herein, a simple cascade enzyme system developed, termed hemin–micelles–GOx, based on hemin glucose oxidase (GOx)‐encapsulated Pluronic F127 (F127) micelles with pH‐sensitive enzymatic activities. Histidine‐tagged GOx can be easily chelated hemin‐F127 via coordination histidine ferrous ions in center by admixture aqueous solution. In tumor microenvironment (TME), hemin–micelles–GOx exhibits enhanced peroxidase (POD)‐like activities generate toxic hydroxyl radicals due acidic condition, whereas catalase (CAT)‐like, not POD‐like activity amplified, resulting elimination hydrogen peroxide oxygen. murine melanoma model, significantly suppresses growth, demonstrating its great potential as pH‐mediated switch management

Language: Английский

Citations

4
Molecularly Imprinted Nanozymes with Substrate Specificity: Current Strategies and Future Direction DOI
Zhou Zhang, Ergui Luo, Wenjuan Wang

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Abstract Molecular imprinting technology (MIT) stands out for its exceptional simplicity and customization capabilities has been widely employed in creating artificial antibodies that can precisely recognize efficiently capture target molecules. Concurrently, nanozymes have emerged as promising enzyme mimics the biomedical field, characterized by their remarkable stability, ease of production scalability, robust catalytic activity, high tunability. Drawing inspiration from natural enzymes, molecularly imprinted combine unique benefits both MIT nanozymes, thereby conferring biomimetic catalysts with substrate specificity selectivity. In this review, latest strategies fabrication focusing on use organic polymers inorganic nanomaterials are explored. Additionally, cutting‐edge techniques generating atom‐layer‐imprinted islands ultra‐thin atomic‐scale thickness is summarized. Their applications particularly noteworthy fields catalyst optimization, detection techniques, therapeutic strategies, where they boost reaction selectivity efficiency, enable precise identification quantification substances, enhance effectiveness while minimizing adverse effects. Lastly, prevailing challenges field delineate potential avenues future progress encapsulated. This review will foster advancements expand applications.

Language: Английский

Citations

4
Tumor microenvironment–responsive and modulatory manganese-based nanoenzyme for enhanced tumor immunotherapy DOI Creative Commons

Qi Yang,

Qiong Wu, Haiyan Liu

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 3, 2025

The characteristics of the tumor microenvironment (TME) have a close and internal correlation with effect cancer immunotherapy, significantly affecting progression metastasis cancer. rational design nanoenzymes that possess ability to respond regulate TME is driving new direction in catalytic immunotherapy. In this study, we designed multifunctional manganese (Mn)-based nanoenzyme responsive acidic pH overxpressed H2O2 at site holds capability modulating hypoxic immunosuppressive for synergistic anti-tumor photothermal/photodynamic/immunotherapy. We found artificial promoted peroxidase-like catalase-like activities catalyzed in-situ decomposition H2O2, metabolic waste product TME, into ∙OH O2, resulting ROS burst killing tumors relieving enhance therapy. Besides photothermal enhancement burst-induced immunogenic cell death, combination Mn2+ released from Mn-based programmed death-ligand 1 blockade triggered significant immune response. A remarkable vivo therapeutic was achieved effective inhibition primary growth lung metastasis. Therefore, TME-responsive offers safe efficient platform reversing achieving

Language: Английский

Citations

0
Strategies for engineering biomimetic materials for tumor therapy DOI
Zhaoyu Ma, Kai Zhang,

Yanli Zhao

et al.

Matter, Journal Year: 2025, Volume and Issue: 8(1), P. 101899 - 101899

Published: Jan. 1, 2025

Language: Английский

Citations

0
Recent Advancements and Future Prospects of Asymmetrically Coordinated Single-Atom Nanozymes with High Enzymatic-like Activity for Biotherapy and Diagnosis: A Review DOI
Changli Chen,

Gangzhi Qin,

Xinyue Geng

et al.

ACS Applied Nano Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 26, 2025

Language: Английский

Citations

0
Catalytic Hybrid Lipid Nanoparticles Potentiate Circle RNA-Based Cytokine Immunotherapy DOI

Yongcan Li,

John Kevin Gan,

Jiaqi Lei

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Cytokine therapeutics in cancer immunotherapy are greatly limited by their short half-time, serious toxicity, and frequent administration, which can possibly be addressed ribonucleic acid (RNA) technology through the expression of targeting cytokines situ. However, intracellular translation RNA remains restricted due to generation excessive reactive oxygen species (ROS) overconsumption adenosine triphosphate (ATP) within transfected cells. Herein, hybrid lipid nanoparticles (Mn-LNPs) developed incorporating small-sized trimanganese tetraoxide conventional nanoparticles, showing ability generate oxygen, eliminate ROS, boost ATP, thus enhancing efficiency. This platform is employed encapsulate interleukin 12 (IL-12)-encoding circular (Mn-LNPs@RNAIL-12) for tumor immunotherapy, exhibiting unparalleled advantages proliferation cytotoxic T cells stimulation antitumor immunity. Moreover, efficacy Mn-LNPs@RNAIL-12 further strengthened synergizing with immune checkpoint blockade therapy achieve durable potent performances.

Language: Английский

Citations

0
Sensitive colorimetric sensing of dopamine and TYR based on enhanced HRP-like activity of CuNi/Fe LDHs nanozymes DOI

Fenghua Geng,

Min Huang, Xiaoli Zhang

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(3)

Published: March 1, 2025

Language: Английский

Citations

0
Dopant‐Regulated Piezocatalysts Evoke Sonopiezoelectric and Enzymatic PANoptosis for Synergistic Cancer Therapy DOI Creative Commons
Linhong Zhong, Xun Guo, Liming Deng

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Piezocatalyst-enabled sonopiezoelectric therapy offers noninvasive treatment with high spatiotemporal selectivity, yet existing piezocatalysts are limited by suboptimal efficacy, cancer cell resistance to oxidative stress, and biosafety concerns. Here, hafnia (HfO2), one of the only few FDA-approved inorganic nanomaterials for clinical trials, is identified as a promising piezocatalyst translational potential enzymatic PANoptosis-boosted nanocatalytic therapy. Specifically, engineered transition metal-substituted HfO2 nanocatalysts synthesized optimize piezoelectric enzyme-mimicking activities. Among these, Mn-substituted 20% Mn ratio (HMO) demonstrates superior performance in sono-triggered reactive oxygen species generation, attributed its reduced bandgap increased vacancies. HMO also exhibits multiple activities, including peroxidase (POD), catalase (CAT), glutathione (GPx), amplifying stress through tumor-specific catalytic reactions. These dual effects enable activation PANoptosis elicit robust antitumor immune response. Biological evaluations show significant tumor suppression responses HMO-mediated Unlike utilizing radiosensitization ability clinic, this work unveils distinctive effect multienzymatic activities HfO2-based biomedical applications, holding overcome challenges radiation damage associated radiotherapy.

Language: Английский

Citations

0
Construction of nanozyme based with mixed valence manganese oxide loaded on defective metal-organic frameworks for sensitive detection of biomarker procalcitonin DOI

Suimin Deng,

Yun Hao, Lina Yang

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117339 - 117339

Published: March 11, 2025

Language: Английский

Citations

0
A Self‐Priming Pyroptosis‐Inducing Agent for Activating Anticancer Immunity DOI Open Access
Yanjie Li, Hanxin Feng, Yang Huang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract Pyroptosis, a form of programmed cell death mediated by the gasdermin family, has emerged as promising strategy for inducing anti‐tumor immunity. However, efficiently pyroptosis in tumor cells remains significant challenge due to limited activation key mediators like caspases tissues. Herein, self‐priming pyroptosis‐inducing agent (MnNZ@OMV) is developed integrating outer membrane vesicles (OMVs) with manganese dioxide nanozymes (MnNZ) trigger cells. OMVs, derived from Escherichia coli , are coated onto spiny MnNZ prepare MnNZ@OMV. Once internalized cells, MnNZ@OMV responds elevated intracellular glutathione (GSH) levels, releasing Mn 2 ⁺ and OMV components. This leads GSH depletion ⁺‐catalyzed reactive oxygen species generation, which triggers NF‐κB translocation prime caspase‐11 expression. Subsequently, lipopolysaccharides OMVs activate caspase‐11, resulting GSDMD cleavage induction. significantly induces vivo, promoting dendritic maturation CD8⁺ T activation, leading robust effects. Collectively, this study presents novel approach through noncanonical caspase‐11/GSDMD pathway, offering avenue future cancer immunotherapy.

Language: Английский

Citations

0