Innovative mutant screening identifiesTRANSPARENT TESTA7as a player in seed oil/protein partitioning DOI Open Access
Alain Lécureuil, Massimiliano Corso, Stéphanie Boutet

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Summary Brassicaceae species mainly accumulate oil and protein in their seeds, which are essential to human life as a source of food, but also animal feed resources for green chemistry. To date, crops such rapeseed have been selected content. However, there is growing interest seed A strong negative correlation between content makes it difficult increase both compounds simultaneously. In this study, an Arabidopsis thaliana homozygous EMS mutant library was screened by near-infrared spectroscopy content, with the aim identifying mutants impaired oil-protein correlation. The most affected found be TRANSPARENT TESTA7 gene, involved flavonoid biosynthetic pathway. Analysis different pathway revealed that tt7 were only ones show significant reduction highlighting phenotype never described before suggesting specific role TT7 interplay pathways. Untargeted metabolomic analysis allowed identification metabolic features highly accumulated seeds compared other genotypes genetic established accumulation kaempferol-3-O-rhamnoside seems responsible mutants. Significance Statement understanding how partitioning these regulated necessary engineer purposes. By screening library, we identified oil/protein partitioning, including , link pathways, explore further paper.

Language: Английский

Salicylic acid: Homeostasis, signalling and phytohormone crosstalk in plants under environmental challenges DOI
Akbar Ali, Krishan Kant, Navneet Kaur

et al.

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 169, P. 314 - 335

Published: May 1, 2024

Language: Английский

Citations

12
Multi‐omic analyses unveil contrasting composition and spatial distribution of specialized metabolites in seeds of Camelina sativa and other Brassicaceae DOI Open Access
Léa Barreda,

Céline Brosse,

Stéphanie Boutet

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(3)

Published: Feb. 1, 2025

SUMMARY Seeds of Brassicaceae produce a large diversity beneficial and antinutritional specialized metabolites (SMs) that influence their quality provide resistance to stresses. While SM distribution has been described in leaves root tissues, limited information is available about spatiotemporal accumulation seeds. Camelina sativa (camelina) an oilseed cultivated for human animal nutrition industrial uses. we previously explored plasticity, no expression related proteins genes camelina In this study, used multi‐omic approach, integrating untargeted metabolomics, proteomics, transcriptomics investigate the synthesis, modification, degradation SMs accumulated seed tissues (seed coat, endosperm, embryo) at six developmental two germination stages. Metabolomic results showed distinct patterns pathways, highlighting significant contrasts composition spatial defense‐related glucosinolate (GSL) compounds among camelina, Arabidopsis thaliana , Brassica napus three closely species. Notably, thanks metabolomic proteomic/transcriptomic techniques variation GSL distributions was primarily driven by differences structure (metabolomics data) transport (transcriptomic proteomic mechanisms. Long‐chain C8–C11 methylsulfinylalkyl GSLs were predominantly coat while mid‐ short‐chain C3–C7 embryo. Characterizing dynamics provides valuable insights can guide development crops with optimized toxic metabolites, improving nutritional profiles.

Language: Английский

Citations

1
Specialized metabolome and transcriptome atlas of developing Arabidopsis thaliana seed under warm temperatures DOI Creative Commons
Léa Barreda, Stéphanie Boutet, Delphine De Vos

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Feb. 20, 2025

Seed development, which depends on parent plants genetic background and mother plant environmental conditions, is a major component determining seed composition. quality main agricultural concern, impacting both food non-food applications, while also playing central role in biodiversity conservation environment protection. Climate change, particular the emergence of extremely high temperatures, constitute critical global threat to agriculture. Specialized metabolites (SMs) play crucial roles interactions seeds with their environments. Several SMs are known be protective compounds involved stress responses, thus quality. In this study, we performed untargeted metabolomic (LC-MS/MS) transcriptomic (RNA-Seq) analyses Arabidopsis thaliana harvested at six developmental stages (Globular, Transition, Torpedo, Bent cotyledon, Mature green Dry seed), developed under control warm temperature conditions. Those data provide an original valuable resource that could used identify genes heat responses for study regulation functions during development.

Language: Английский

Citations

0
Deciphering seed development and germination in the single-cell era DOI Creative Commons
Paul Schouveiler, Lim Chee Liew, Massimiliano Corso

et al.

Seed Science Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10

Published: March 3, 2025

Abstract Seeds are complex structures that serve as dispersal units in angiosperms. consist of three specialized tissues with distinct roles and molecular compositions. Hence, the characterization genetic regulators act within individual seed tissues, how their activity changes during development germination, has been a primary focus research. However, our knowledge spatiotemporal modulation seeds, across different cell types, limited by resolution available techniques. In last few years, application single-cell technologies plants have enabled elucidation gene networks involved various developmental processes at cellular level. Some studies applied these to enabling further germination Here, we review current status seeds present workflow for conducting transcriptomics. Additionally, discuss integration multi-omics, aiming demonstrate potential enhancing comprehension regulations governing germination.

Language: Английский

Citations

0
Phylogenomic and synteny analysis of BAHD and SCP/SCPL gene families reveal their evolutionary histories in plant specialized metabolism DOI Creative Commons
Thomas Naake, John C. D’Auria, Alisdair R. Fernie

et al.

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1914)

Published: Sept. 29, 2024

Plant chemical diversity is largely owing to a number of enzymes which catalyse reactions involved in the assembly, and subsequent modifications, core structures major classes plant specialized metabolites. One such reaction acylation. With this mind, study deep evolutionary history BAHD serine-carboxypeptidase-like (SCPL) acyltransferase genes, we assembled phylogenomic synteny networks based on large-scale inference analysis orthologues across whole-genome sequences 126 species spanning Stramenopiles Archaeplastida, including Arabidopsis thaliana , tomato ( Solanum lycopersicum ) maize Zea mays ). As such, combined genomic location with changes gene sequences. Our analyses revealed that serine-carboxypeptidase (SCP)/serine-carboxypeptidase-like genes had deeper origin than expanded massively transition land development vascular system. The two families additionally display quite distinct patterns copy variation phylogenies as well differences cross-phylogenetic syntenic network components. In unlocking above observations, our demonstrate possibilities afforded by modern (syntenic) networks, but also highlight their current limitations, demonstrated inability phylogenetic methods separate authentic SCPL acyltransferases from standard SCP peptide hydrolases. This article part theme issue ‘The evolution metabolism’.

Language: Английский

Citations

2
Multi-omic analyses unveil temporal and spatial distribution of specialized metabolites in seeds ofCamelina sativa DOI Creative Commons
Léa Barreda,

Céline Brosse,

Stéphanie Boutet

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 6, 2024

Abstract Seeds of Brassicaceae species produce a large diversity specialized metabolites (SMs) that strongly influence their quality, with beneficial or toxic effects on human and animal nutrition, provide resistance to biotic abiotic stresses. While the distribution these compounds has been described in leaves roots tissues, very limited information is available about spatio-temporal accumulation seeds model crop plants. Camelina sativa (camelina) an oilseed cultivated for industrial uses. we previously explored detail SM plasticity, no expression SM-related proteins genes camelina seeds. In this study used untargeted metabolomics (LC-MS/MS), proteomics (DIA) transcriptomics (RNA-Seq) analyse synthesis, transport, modifications degradations SMs are accumulated different seed tissues (i.e. coat, endosperm, embryo) at 6 developmental 2 germination stages. Our results showed specific patterns many SMs, related genes, during coat embryo development. We also that, differently from Arabidopsis thaliana seeds, defence antinutritional glucosinolates were both corresponding degradation products isothiocyanates present high level embryos dry C. . Characterizing spatial dynamics will contribute development crops optimized quality nutrition.

Language: Английский

Citations

0
Innovative mutant screening identifiesTRANSPARENT TESTA7as a player in seed oil/protein partitioning DOI Open Access
Alain Lécureuil, Massimiliano Corso, Stéphanie Boutet

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Summary Brassicaceae species mainly accumulate oil and protein in their seeds, which are essential to human life as a source of food, but also animal feed resources for green chemistry. To date, crops such rapeseed have been selected content. However, there is growing interest seed A strong negative correlation between content makes it difficult increase both compounds simultaneously. In this study, an Arabidopsis thaliana homozygous EMS mutant library was screened by near-infrared spectroscopy content, with the aim identifying mutants impaired oil-protein correlation. The most affected found be TRANSPARENT TESTA7 gene, involved flavonoid biosynthetic pathway. Analysis different pathway revealed that tt7 were only ones show significant reduction highlighting phenotype never described before suggesting specific role TT7 interplay pathways. Untargeted metabolomic analysis allowed identification metabolic features highly accumulated seeds compared other genotypes genetic established accumulation kaempferol-3-O-rhamnoside seems responsible mutants. Significance Statement understanding how partitioning these regulated necessary engineer purposes. By screening library, we identified oil/protein partitioning, including , link pathways, explore further paper.

Language: Английский

Citations

0