Serum SARM1 Levels and Diabetic Peripheral Neuropathy in Type 2 Diabetes: Correlation with Clinical Neuropathy Scales and Nerve Conduction Studies and Impact of COVID-19 vaccination DOI Creative Commons
Moafaq S. Alrawaili, Ahmad R. Abuzinadah,

Aysha A. Alshareef

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(2), P. 209 - 209

Published: Feb. 17, 2024

Patients with peripheral neuropathy type 2 diabetes mellitus (T2DM) are more likely to have functional impairments. Recently, the gene for serum sterile alpha and toll/interleukin receptor motif-containing protein 1 (SARM1), which may contribute pathogenesis of Wallerian degeneration, was discovered in mice models neuropathy. We set out assess SARM1's activity as a potential biomarker early identification diabetic T2DM patients while also examining impact COVID-19 vaccine on SARM1 levels. assessed cross-sectional relationships between biomarker, clinical scales, nerve conduction parameters 80 participants aged 30 years 60 years. The analysis carried after were split into two groups since we significant increase levels following second dose vaccination, where group A received one inoculation, B doses vaccine. correlated significantly (

Language: Английский

The mechanism underlying extrapulmonary complications of the coronavirus disease 2019 and its therapeutic implication DOI Creative Commons
Qin Ning, Di Wu, Xiaojing Wang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2022, Volume and Issue: 7(1)

Published: Feb. 23, 2022

Abstract The coronavirus disease 2019 (COVID-19) is a highly transmissible caused by the severe acute respiratory syndrome 2 (SARS-CoV-2) that poses major threat to global public health. Although COVID-19 primarily affects system, causing pneumonia and distress in cases, it can also result multiple extrapulmonary complications. pathogenesis of damage patients with probably multifactorial, involving both direct effects SARS-CoV-2 indirect mechanisms associated host inflammatory response. Recognition features complications has clinical implications for identifying progression designing therapeutic strategies. This review provides an overview from immunological pathophysiologic perspectives focuses on potential targets management COVID-19.

Language: Английский

Citations

72
NAD+ in COVID-19 and viral infections DOI Open Access
Minyan Zheng, Michael Schultz, David Sinclair

et al.

Trends in Immunology, Journal Year: 2022, Volume and Issue: 43(4), P. 283 - 295

Published: Feb. 11, 2022

Language: Английский

Citations

43
COVID‐19 metabolism: Mechanisms and therapeutic targets DOI
Tianshi Wang, Ying Cao, Haiyan Zhang

et al.

MedComm, Journal Year: 2022, Volume and Issue: 3(3)

Published: Aug. 9, 2022

Abstract Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) dysregulates antiviral signaling, immune response, and cell metabolism in human body. Viral genome proteins hijack host metabolic network to support viral biogenesis propagation. However, the regulatory mechanism of SARS‐CoV‐2‐induced dysfunction has not been elucidated until recently. Multiomic studies disease 2019 (COVID‐19) revealed an intensive interaction between regulators proteins. SARS‐CoV‐2 deregulated cellular blood, intestine, liver, pancreas, fat, cells. Host supported almost every stage lifecycle. Strikingly, were found interact with enzymes different compartments. Biochemical genetic assays also identified key nodes dependencies replication. Of note, cholesterol metabolism, lipid glucose are broadly involved Here, we summarized current understanding hallmarks COVID‐19 metabolism. infection remodels which turn modulates Remodeling creates vulnerability replication, could be explored uncover new therapeutic targets. The efficacy inhibitors against is under investigation several clinical trials. Ultimately, knowledge reprogramming would accelerate drug repurposing or screening combat pandemic.

Language: Английский

Citations

35
Redox Regulation and Metabolic Dependency of Zika Virus Replication: Inhibition by Nrf2-Antioxidant Response and NAD(H) Antimetabolites DOI
Bikash R. Sahoo, Alexandra A. Crook, Aryamav Pattnaik

et al.

Journal of Virology, Journal Year: 2023, Volume and Issue: 97(2)

Published: Jan. 23, 2023

Zika virus (ZIKV) is a major public health concern of international proportions. While the incidence ZIKV infections has declined substantially in recent years, potential for reemergence or reintroduction remains high.

Language: Английский

Citations

15
Computational drug repurposing for viral infectious diseases: a case study on monkeypox DOI
Sovan Saha, Piyali Chatterjee, Mita Nasipuri

et al.

Briefings in Functional Genomics, Journal Year: 2024, Volume and Issue: 23(5), P. 570 - 578

Published: Jan. 5, 2024

The traditional method of drug reuse or repurposing has significantly contributed to the identification new antiviral compounds and therapeutic targets, enabling rapid response developing infectious illnesses. This article presents an overview how modern computational methods are used in for treatment viral diseases. These utilize data sets that include reviewed information on host's pathogens drugs, as well various connections such gene expression patterns protein-protein interaction networks. We assess potential benefits limitations these by examining monkeypox a specific example, but knowledge acquired can be applied other comparable disease scenarios.

Language: Английский

Citations

4
Bacterial metabolism in the host and its association with virulence DOI Creative Commons

Amrita C. Bhagwat,

Tiyasa Haldar,

Poonam Kanojiya

et al.

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 31, 2025

The host restricted pathogens are competently dependent on their respective for nutritional requirements. bacterial metabolic pathways surprisingly varied and remarkably flexible that in turn help them to successfully overcome competition colonise host. adaptation plays pivotal role pathogenesis. understanding of host-pathogen crosstalk needs be prioritized decipher interactions. review focuses various aspects pathogen interactions majorly involves metabolism counteract immune mechanisms by rectifying cues provides the idea different anatomical sites local physiology key set metabolites recognized as centre between its also briefly discussed. factors control timely expression virulence is poorly understood. perspective presented herein will facilitate us with a broader view molecular modulates pathogens. knowledge crosslinked bacteria serve develop novel potential therapeutics.

Language: Английский

Citations

0
Apprehending the NAD+–ADPr-Dependent Systems in the Virus World DOI Creative Commons
Lakshminarayan M. Iyer, A. Maxwell Burroughs, Vivek Anantharaman

et al.

Viruses, Journal Year: 2022, Volume and Issue: 14(9), P. 1977 - 1977

Published: Sept. 7, 2022

NAD+ and ADP-ribose (ADPr)-containing molecules are at the interface of virus-host conflicts across life encompassing RNA processing, restriction, lysogeny/dormancy functional hijacking. We objectively defined central components NAD+-ADPr networks involved in these systematically surveyed 21,191 completely sequenced viral proteomes representative all publicly available branches world to reconstruct a comprehensive picture systems. These systems have been widely repeatedly exploited by positive-strand DNA viruses, especially those with larger genomes more intricate life-history strategies. present evidence that ADP-ribosyltransferases (ARTs), ADPr-targeting Macro, NADAR Nudix proteins frequently packaged into virions, particularly phages contractile tails (Myoviruses), deployed during infection modify host macromolecules counter NAD+-derived signals restriction. Genes encoding NAD+-ADPr-utilizing domains were exchanged between distantly related hosts endo-parasites/symbionts, suggesting selection for them virus world. Contextual analysis indicates bacteriophage versions likely soluble ADPr derivatives, while eukaryotic might prefer macromolecular adducts. Finally, we also use comparative genomics predict countering ADP ribosylation molecules.

Language: Английский

Citations

17
Environmental desiccation stress induces viable but non culturable state in Neisseria meningitidis DOI
Poonam Kanojiya,

Tiyasa Haldar,

Sunil D. Saroj

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(2)

Published: Jan. 29, 2025

Language: Английский

Citations

0
Enteric coronavirus PDCoV evokes a non-Warburg effect by hijacking pyruvic acid as a metabolic hub DOI Creative Commons

Guanning Su,

Jiao Liu,

Chenrui Duan

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 71, P. 103112 - 103112

Published: March 4, 2024

The Warburg effect, also referred as aerobic glycolysis, is a common metabolic program during viral infection. Through targeted metabolomics combined with biochemical experiments and various cell models, we investigated the central carbon metabolism (CCM) profiles of cells infected porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus zoonotic potential. We found that PDCoV infection required glycolysis but decreased glycolytic flux, exhibiting non-Warburg effect characterized by pyruvic acid accumulation. Mechanistically, enhanced pyruvate kinase activity to promote anabolism, process generates concomitant ATP production. hijacked catabolism increase biosynthesis non-essential amino acids (NEAAs), suggesting essential hub for scavenge host energy metabolites. Furthermore, facilitated glutaminolysis synthesis NEAA pyrimidines optimal proliferation. Our work supports novel CCM model after provides potential anti-PDCoV drug targets.

Language: Английский

Citations

3
Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated Substrates DOI Open Access

Chanbora Chea,

Duck‐Yeon Lee, Jiro Kato

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 7, 2023

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for a global pandemic that resulted in more than 6-million deaths worldwide. The virus encodes several non-structural proteins (Nsps) contain elements capable of disrupting cellular processes. Among these Nsp proteins, Nsp3 contains macrodomains, e.g., Mac1, Mac2, Mac3, with potential effects on host cells. Mac1 has been shown to increase SARS-CoV-2 virulence and disrupt ADP-ribosylation pathways mammalian results from the transfer ADP-ribose moiety NAD + various acceptors, DNA, RNA, contributing cell's biological mechanism action bacterial toxins, Pseudomonas diphtheria toxin protein biosynthetic signaling pathways. On other hand, some viral macrodomains cleavage ADP-ribose-acceptor bond, generating free ADP-ribose. By this reaction, macrodomain-containing interfere homeostasis Here, we examined hydrolytic activities 2, 3 substrates containing cleaved α-NAD , but not β-NAD consistent stereospecificity at C-1" bond. In contrast ARH1 ARH3, did require Mg 2+ optimal activity. also hydrolyzed O -acetyl-ADP-ribose ADP-ribose-1"-phosphat, Mac2 Mac3. However, cleave α-ADP-ribose-(arginine) ADP-ribose-(serine)-histone H3 peptide, suggesting hydrolyzes attached O- N-linked functional groups, specificity catalytic site moiety. We conclude may exert anti-viral activity by reversing host-mediated ADP-ribosylation. New insights shed light therapeutic targets.SARS-CoV-2, COVID-19, within (Nsp3). was previously hydrolyze ADP-ribose-phosphate. Inactivation reduced proliferation. Here report multiple activities, i.e., hydrolyzed. -acetyl-ADP-ribose. ADP-ribose-serine histone peptide (aa1-21), ADP-ribose-arginine, exhibiting substrate selectivity. These data suggest have multi-function as consumer replication disruptor Understanding Mac1's mechanisms important provide possible targets COVID-19.

Language: Английский

Citations

6