Optimizing Ge Enrichment in Lyophyllum decastes Fermentation for Enhanced Biological Activity DOI Creative Commons

Sher Hassan,

Jinyan Zhao,

Sana Tahir

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 641 - 641

Published: Dec. 14, 2024

This study enhanced germanium (Ge) enrichment in the liquid fermentation of edible fungus Lyophyllum decastes order to boost its biological activity, particularly antioxidant and immunomodulatory properties. Through use single-factor Plackett–Burman designs, experiments revealed critical parameters affecting Ge enrichment, including oxide concentration, potato powder peptone levels. The optimization Box–Behnken response surface methodology resulted a concentration 3.61 mg/L, significantly enhancing biomass, protein, polysaccharide, flavonoid content mycelium. In contrast traditional fermentation, Ge-rich mycelial biomass by 30.97% elevated organic 50.19-fold. An analysis antioxidants that Ge-enriched water extract exhibited heightened augmenting TNF-α production RAW264.7 cells 73.29% at 200 μg/g. These findings indicate L. holds promise for functional applications health-supportive products due robust immune-enhancing capabilities.

Language: Английский

Multi-Target Inhibition of Streptococcus pyogenes Skin Infections by Officinal Magnolia Bark Rhubarb Decoction: Network Pharmacology and Molecular Docking Insights DOI Creative Commons

yuanhao wang,

Xinrui Wang, Xueying Zhang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract Streptococcus pyogenes infection still poses a great threat to humanity on global scale. The emergence of antibiotic resistance endangers health care systems worldwide. Unlike single component antibiotics, traditional Chinese medicine exerts its effects through multiple pathways and targets, thereby reducing the chance bacteria developing resistance. main components in Officinal magnolia bark rhubarb Decoction, namely bark, Rhubarb Immature Bitter Orange, have been proven antibacterial previous studies. In this study, network pharmacology molecular docking, interactions between active targets related skin were determined, enrichment analysis was carried out. Molecular docking studies conducted 15 screened drug 12 target proteins. Finally, vitro experiments used prove that Decoction can inhibit growth treat infections.

Language: Английский

Citations

0
Optimizing Ge Enrichment in Lyophyllum decastes Fermentation for Enhanced Biological Activity DOI Creative Commons

Sher Hassan,

Jinyan Zhao,

Sana Tahir

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 641 - 641

Published: Dec. 14, 2024

This study enhanced germanium (Ge) enrichment in the liquid fermentation of edible fungus Lyophyllum decastes order to boost its biological activity, particularly antioxidant and immunomodulatory properties. Through use single-factor Plackett–Burman designs, experiments revealed critical parameters affecting Ge enrichment, including oxide concentration, potato powder peptone levels. The optimization Box–Behnken response surface methodology resulted a concentration 3.61 mg/L, significantly enhancing biomass, protein, polysaccharide, flavonoid content mycelium. In contrast traditional fermentation, Ge-rich mycelial biomass by 30.97% elevated organic 50.19-fold. An analysis antioxidants that Ge-enriched water extract exhibited heightened augmenting TNF-α production RAW264.7 cells 73.29% at 200 μg/g. These findings indicate L. holds promise for functional applications health-supportive products due robust immune-enhancing capabilities.

Language: Английский

Citations

1