Alleviation of thermal nociception depends on heat-sensitive neurons and a TRP channel in the brain DOI Creative Commons

Jiangqu Liu,

Weiwei Liu, Dhananjay Thakur

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(12), P. 2397 - 2406.e6

Published: May 17, 2023

Acute avoidance of dangerous temperatures is critical for animals to prevent or minimize injury. Therefore, surface receptors have evolved endow neurons with the capacity detect noxious heat so that can initiate escape behaviors. Animals including humans intrinsic pain-suppressing systems attenuate nociception under some circumstances. Here, using Drosophila melanogaster, we uncovered a new mechanism through which thermal suppressed. We identified single descending neuron in each brain hemisphere, center suppression nociception. These Epi neurons, Epione—the goddess soothing pain—express nociception-suppressing neuropeptide Allatostatin C (AstC), related mammalian anti-nociceptive peptide, somatostatin. are direct sensors heat, and when activated they release AstC, diminishes found also express heat-activated TRP channel, Painless (Pain), activation subsequent depend on Pain. Thus, while channels well known sense promote behavior, this work reveals first role channel detecting purpose suppressing rather than enhancing behavior response hot stimuli.

Language: Английский

A Drosophila computational brain model reveals sensorimotor processing DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Nico Spiller

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 210 - 219

Published: Oct. 2, 2024

Language: Английский

Citations

24
Thermal infrared directs host-seeking behaviour in Aedes aegypti mosquitoes DOI Creative Commons
Avinash Chandel, Nicolas A. DeBeaubien, Anindya Ganguly

et al.

Nature, Journal Year: 2024, Volume and Issue: 633(8030), P. 615 - 623

Published: Aug. 21, 2024

Mosquito-borne diseases affect hundreds of millions people annually and disproportionately impact the developing world

Language: Английский

Citations

20
Ir56b is an atypical ionotropic receptor that underlies appetitive salt response in Drosophila DOI Creative Commons
Hany K. M. Dweck, Gaëlle J.S. Talross, Yichen Luo

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(8), P. 1776 - 1787.e4

Published: March 15, 2022

Language: Английский

Citations

57
Neural Circuits Underlying Behavioral Flexibility: Insights From Drosophila DOI Creative Commons
Anita V. Devineni, Kristin M. Scaplen

Frontiers in Behavioral Neuroscience, Journal Year: 2022, Volume and Issue: 15

Published: Jan. 6, 2022

Behavioral flexibility is critical to survival. Animals must adapt their behavioral responses based on changes in the environmental context, internal state, or experience. Studies Drosophila melanogaster have provided insight into neural circuit mechanisms underlying flexibility. Here we discuss how behavior modulated by and learning. We describe general principles of organization modulation that underlie flexibility, are likely extend other species.

Language: Английский

Citations

41
Avoiding cantharidin through ionotropic receptors DOI
Roshani Nhuchhen Pradhan, Bhanu Shrestha, Youngseok Lee

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 466, P. 133497 - 133497

Published: Jan. 11, 2024

Language: Английский

Citations

13
Evolution of chemosensory tissues and cells across ecologically diverse Drosophilids DOI Creative Commons
Gwénaëlle Bontonou, Bastien Saint‐Leandre, Tane Kafle

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 5, 2024

Abstract Chemosensory tissues exhibit significant between-species variability, yet the evolution of gene expression and cell types underlying this diversity remain poorly understood. To address these questions, we conducted transcriptomic analyses five chemosensory from six Drosophila species integrated findings with single-cell datasets. While stabilizing selection predominantly shapes transcriptomes, thousands genes in each tissue have evolved differences. Genes that changed one often multiple other but at different past epochs are more likely to be type-specific than unchanged genes. Notably, chemosensory-related undergone widespread changes, numerous species-specific gains/losses including novel chemoreceptors patterns. Sex differences also pervasive, a D. melanogaster- specific excess male-biased sensory muscle cells its forelegs. Together, our provide new insights for understanding evolutionary changes both global individual levels.

Language: Английский

Citations

12
Mechanism for food texture preference based on grittiness DOI Creative Commons
Qiaoran Li, Craig Montell

Current Biology, Journal Year: 2021, Volume and Issue: 31(9), P. 1850 - 1861.e6

Published: March 2, 2021

An animal's decision to accept or reject a prospective food is based only, in part, on its chemical composition. Palatability also greatly influenced by textural features including smoothness versus grittiness, which particle sizes. Here, we demonstrate that Drosophila melanogaster endowed with the ability discriminate sizes and uses this information decide whether appealing. The depends mechanically activated channel, OSCA/TMEM63, conserved from plants humans. We found tmem63 expressed multidendritic neuron (md-L) fly tongue. Loss of impairs activation md-L mechanical stimuli choose size. These findings reveal first role for evolutionarily conserved, TMEM63 channel an animal provide explanation how flies can sense behaviorally respond texture provided particles.

Language: Английский

Citations

54
Taste quality and hunger interactions in a feeding sensorimotor circuit DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Stefanie Engert

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: July 6, 2022

Taste detection and hunger state dynamically regulate the decision to initiate feeding. To study how context-appropriate feeding decisions are generated, we combined synaptic resolution circuit reconstruction with targeted genetic access specific neurons elucidate a gustatory sensorimotor for initiation in adult Drosophila melanogaster. This connects sensory proboscis motor through three intermediate layers. Most this pathway necessary sufficient extension, behavior, respond selectively sugar taste detection. Pathway activity is amplified by signals that act at select second-order promote food-deprived animals. In contrast, inhibited bitter impinges on premotor neurons, illuminating local motif weighs adjust behavioral outcomes. Together, these studies reveal central mechanisms integration of external internal nutritive flexibly execute critical decision.

Language: Английский

Citations

38
Meeting a threat of the Anthropocene: Taste avoidance of metal ions by Drosophila DOI Creative Commons
Shuke Xiao, Lisa Soyeon Baik,

Xueying Shang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(25)

Published: June 14, 2022

The Anthropocene Epoch poses a critical challenge for organisms: they must cope with new threats at rapid rate. These include toxic chemical compounds released into the environment by human activities. Here, we examine elevated concentrations of heavy metal ions as an example anthropogenic stressors. We find that fruit fly Drosophila avoids nine when present flies experienced rarely, if ever, until Anthropocene. characterize avoidance feeding and egg laying on ions, identify receptors, neurons, taste organs contribute to this avoidance. Different subsets including members both Ir (Ionotropic receptor) Gr (Gustatory families different ions. activate certain bitter-sensing neurons inhibit sugar-sensing neurons. Some behavioral responses are mediated largely through pharynx. Feeding remains stable over 10 generations exposure copper zinc conserved across diverse dipteran species, mosquito Aedes albopictus . Our results suggest mechanisms may be essential insects face challenges from environmental changes in

Language: Английский

Citations

35
Mutagenesis of the odorant receptor co-receptor (Orco) reveals severe olfactory defects in the crop pest moth Helicoverpa armigera DOI Creative Commons

Xiao-Bin Fan,

Bao‐Tong Mo,

Guo‐Cheng Li

et al.

BMC Biology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Sept. 30, 2022

Abstract Background Odorant receptors (ORs) as odorant-gated ion channels play a crucial role in insect olfaction. They are formed by heteromultimeric complex of the odorant receptor co-receptor (Orco) and ligand-selective Or. Other types olfactory proteins, such ionotropic (IRs) some gustatory (GRs), also involved system insects. Orco an obligatory subunit ORs is highly conserved, providing opportunity to systematically evaluate OR-dependent responses. Results Herein, we successfully established homozygous mutant ( −/− ) Helicoverpa armigera , notorious crop pest, using CRISPR/Cas9 gene editing technique. We then compared response characteristics wild type (WT) adults larvae. males were infertile, while females fertile. The lifespan was longer than that WT females. expressions most Ors Irs other olfaction-related genes adult antennae moths not obviously affected, but them up- or down-regulated. In addition, there no change neuroanatomical phenotype at level antennal lobe (including macroglomerular region male). Using EAG SSR techniques, discovered electrophysiological responses sex pheromone components many host plant odorants absent. upwind flight behaviors toward pheromones severely reduced wind tunnel experiment. oviposition selectivity (green pepper) has completely disappeared, chemotaxis green pepper lost Conclusions Our study indicates OR-mediated olfaction essential for communication, selection, larval H. suggesting strategy which mate searching host-seeking moth pests could be disrupted inhibiting silencing expression.

Language: Английский

Citations

31