Current Biology, Journal Year: 2024, Volume and Issue: 34(13), P. R618 - R620
Published: July 1, 2024
Language: Английский
Science, Journal Year: 2022, Volume and Issue: 378(6621)
Published: Nov. 17, 2022
The Hippo signaling pathway is widely considered a master regulator of organ growth because the prominent overgrowth phenotypes caused by experimental manipulation its activity. Contrary to this model, we show here that removing transcriptional output did not impair ability mouse liver and Drosophila eyes grow their normal size. Moreover, activity effectors Yap/Taz/Yki correlate with cell proliferation, hyperactivation these induced gene expression programs recapitulate development. Concordantly, functional screen in identified several target genes were required for ectopic but growth. Thus, does instruct growth, Hippo-induced are activation abnormal genetic programs.
Language: Английский
Citations
55
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(23), P. 15132 - 15132
Published: Dec. 1, 2022
Over a long period of evolution, insects have developed unique intestinal defenses against invasion by foreign microorganisms, including physical and immune responses. The the insect gut consist mainly peritrophic matrix (PM) mucus layer, which are first barriers to pathogens. Gut microbes also prevent colonization Importantly, immune-deficiency (Imd) pathways produce antimicrobial peptides eliminate pathogens; mechanisms related reactive oxygen species another important pathway for immunity. janus kinase/STAT signaling is involved in immunity producing bactericidal substances regulating tissue repair. Melanization can many active into intestine; meanwhile, there multiple responses intestine fight viral parasitic infections. Furthermore, stem cells (ISCs) indispensable Only coordinated combination defense system renewal effectively defend pathogenic microorganisms.
Language: Английский
Citations
47
Current Biology, Journal Year: 2024, Volume and Issue: 34(13), P. 2785 - 2800.e7
Published: May 31, 2024
Host-microbe interactions influence intestinal stem cell (ISC) activity to modulate epithelial turnover and composition. Here, we investigated the functional impacts of viral infection on homeostasis mechanisms by which alters ISC activity. We report that Drosophila A virus (DAV) disrupts in inducing sustained proliferation, resulting dysplasia, loss gut barrier function, reduced lifespan. found additional viruses common laboratory-reared also promote proliferation. The mechanism DAV-induced proliferation involves progenitor-autonomous epidermal growth factor receptor (EGFR) signaling, c-Jun N-terminal kinase (JNK) enterocytes, requires Sting-dependent nuclear κB (NF-κB) (Relish) further demonstrate activating Sting-Relish signaling is sufficient induce reduce lifespan absence infection. Our results reveal can significantly disrupt physiology, highlight a novel role for support aging.
Language: Английский
Citations
9
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 488, P. 151193 - 151193
Published: April 10, 2024
Language: Английский
Citations
7
PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(1), P. e1011823 - e1011823
Published: Jan. 18, 2024
A variety of coordinated host-cell responses are activated as defense mechanisms against pore-forming toxins (PFTs). Bacillus thuringiensis (Bt) is a worldwide used biopesticide whose efficacy and precise application methods limits its use to replace synthetic pesticides in agricultural settings. Here, we analyzed the intestinal two lepidopteran insect pests after intoxication with sublethal dose Bt PFTs find out potential functional genes. We show that larval epithelium was initially damaged by survival observed regeneration. Further analyses showed regeneration caused Cry9A protein regulated through c-Jun NH (2) terminal kinase (JNK) Janus tyrosine kinase/signal transducer activator transcription (JAK/STAT) signaling pathways. JAK/STAT regulates proliferation differentiation stem cells defend three different proteins including Cry9A, Cry1F or Vip3A both pests, Chilo suppressalis Spodoptera frugiperda . Consequently, nano-biopesticide designed improve pesticidal based on combination Stat double stranded RNA (dsRNA)-nanoparticles strain. This formulation controlled better effect suggesting reduce settings for pest control.
Language: Английский
Citations
6
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: Feb. 14, 2024
The midgut, a vital component of the digestive system in arthropods, serves as an interface between ingested food and insect’s physiology, playing pivotal role nutrient absorption immune defense mechanisms. Distinct cell types, including columnar, enteroendocrine, goblet regenerative cells, comprise midgut insects contribute to its robust response. Enterocytes/columnar primary absorptive facilitate response through enzyme secretions, while cells play crucial maintaining integrity by continuously replenishing damaged continuity defense. peritrophic membrane is innate immunity, shielding from pathogens abrasive particles. Midgut juice, mixture enzymes antimicrobial factors, further contributes defense, helping insect combat invading regulate microbial community. cutting-edge single-cell transcriptomics also unveiled previously unrecognized subpopulations within elucidated striking similarities gastrointestinal tracts higher mammals. Understanding intricate interplay types provides valuable insights into immunity. This review solid foundation for unraveling complex roles not only digestion but Moreover, this will discuss novel strategies led employed pathogens, ultimately contributing broader understanding physiology
Language: Английский
Citations
6
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: June 28, 2024
Abstract JNK signaling is a critical regulator of inflammation and regeneration, but how it controlled in specific tissue contexts remains unclear. Here we show that, the Drosophila intestine, TNF-type ligand, Eiger (Egr), expressed exclusively by intestinal stem cells (ISCs) enteroblasts (EBs), where induced stress during aging. Egr preferentially activates paracrine fashion differentiated enterocytes (ECs) via its receptor, Grindelwald (Grnd). N -glycosylation genes ( Alg3 , Alg9 ) restrain this activation, stress-induced downregulation correlates with suggesting regulatory switch. activity ECs induces expression intermembrane protease Rhomboid (Rho), driving secretion EGFR ligands Keren (Krn) Spitz (Spi), which turn activate progenitor (ISCs EBs) to stimulate their growth division, as well produce more Egr. This study uncovers an -glycosylation-controlled, JNK-EGFR-JNK feedforward loop that sustains ISC proliferation gut regeneration.
Language: Английский
Citations
5
Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(4)
Published: Jan. 19, 2024
Pharmacological therapies are promising interventions to slow down aging and reduce multimorbidity in the elderly. Studies animal models first step toward translation of candidate molecules into human therapies, as they aim elucidate molecular pathways, cellular mechanisms, tissue pathologies involved anti-aging effects. Trametinib, an allosteric inhibitor MEK within Ras/MAPK (Ras/Mitogen-Activated Protein Kinase) pathway currently used anti-cancer treatment, emerged a geroprotector because it extended lifespan fruit fly Drosophila melanogaster . Here, we confirm that trametinib consistently robustly extends female lifespan, reduces intestinal stem cell (ISC) proliferation, tumor formation, dysplasia, barrier disruption guts aged flies. In contrast, pro-longevity effects weak inconsistent males, does not influence gut homeostasis. Inhibition specifically ISCs is sufficient partially recapitulate trametinib. Moreover, ISCs, decreases activity RNA polymerase III (Pol III), conserved enzyme synthesizing transfer RNAs other short, non-coding RNAs, whose inhibition also pathology. Finally, show effect mediated by Maf1, repressor Pol III, suggesting life-limiting Ras/MAPK-Maf1-Pol axis these cells. The mechanism action described this work paves way for further studies on mammals shows its potential clinical application humans.
Language: Английский
Citations
4
Foods, Journal Year: 2024, Volume and Issue: 13(8), P. 1140 - 1140
Published: April 9, 2024
Bacillus cereus (Bc) is a wide group of Gram-positive and spore-forming bacteria, known to be the etiological agents various human infections, primarily food poisoning. The Bc includes enteropathogenic strains able germinate in digestive tract produce enterotoxins such as Nhe, Hbl, CytK. One species group, thuringiensis (Bt), has unique feature producing insecticidal crystals during sporulation, making it an important alternative chemical pesticides protect crops from insect pest larvae. Nevertheless, several studies have suggested link between ingestion pesticide cases poisoning, calling their safety into question. Consequently, reliable tools for virulence assessment are worth developing aid decision regulation. Here, we propose complementary approaches based on two biological models, intestinal Caco-2 cell line Drosophila melanogaster, assess rank enteric potency Bt comparison with other members. Using dataset 48 spp. strains, showed that some including Bt, were induce cytotoxicity cells concomitant release IL-8 cytokine, landmark pro-inflammatory response. In D. melanogaster model, sort panel 39 four different classes virulence, ranging no strong virulence. Importantly, most virulent mortality was associated loss barrier integrity. Interestingly, although can share common toxinotype, they display degrees suggesting existence specific mechanisms expression vivo intestine.
Language: Английский
Citations
4
Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 618 - 618
Published: Jan. 31, 2025
Rhamnolipids, biosurfactants synthesized from natural resources, demonstrate significant applications, including notable insecticidal efficacy against Aedes aegypti L., the primary vector for numerous arboviruses. The global spread of A. poses substantial public health challenges, requiring innovative and sustainable control strategies. This research investigates use andiroba (Carapa guianensis Aubl.) biomass waste as a substrate synthesizing rhamnolipid biosurfactant (BSAW) produced by Pseudomonas aeruginosa evaluates its activity aegypti. findings indicate yield 4.42 mg mL-1, alongside an emulsification index approaching 60%. BSAW successfully reduced both surface interfacial tensions to below 30 mN/m 4 mN/m, respectively. Characterization revealed that is di-rhamnolipid, consisting two rhamnose units covalently linked saturated C10 fatty acid chain. At concentration 1.0 exhibited larvicidal activity, leading structural impairments cellular dysfunctions in larvae while also disrupting their associated bacterial microbiota. Moreover, effectively deterred oviposition adult mosquitoes. These underscore BSAW's potential compromise various developmental stages aegypti, supporting integrated arbovirus management approaches. Furthermore, this emphasizes feasibility utilizing agro-industrial substrates microbial production.
Language: Английский
Citations
0