Chromosomal‐level assembly of Bactericera cockerelli reveals rampant gene family expansions impacting genome structure, function and insect‐microbe‐plant‐interactions DOI Creative Commons
Younghwan Kwak,

Jacob A. Argandona,

Patrick H. Degnan

et al.

Molecular Ecology Resources, Journal Year: 2022, Volume and Issue: 23(1), P. 233 - 252

Published: Aug. 4, 2022

Lineage specific expansions and gene duplications are some of the most important sources evolutionary novelty in eukaryotes. Although not as prevalent eukaryotes compared to bacteria, horizontal transfer events can also result key adaptations for insects, especially those involved insect-microbe interactions. In this study we assemble first chromosomal assembly psyllid Bactericera cockerelli reveal that B. genome has experienced significantly more expansion other Hemipteran representatives with fully sequenced genomes. We cockerelli's is largest (567 Mb) date ~15% larger than two species genomes (Pachypsylla venusta Diaphorina citri). Structurally, appears have an additional chromosome distantly related P. due a previous fission or fusion event. The increase size dynamic nature may largely be contributed widespread type I II repeat elements rampant across all cockerelli's. chromosomes. These distributed near equally both euchromatic heterochromatic regions. Furthermore, significant family were uncovered genes expected its adaptation insect-plant microbe interactions, which include transcription factors, proteases, odorant receptors, horizontally transferred nutritional symbioses their long-term endosymbiont Carsonella.

Language: Английский

A versatile depigmentation, clearing, and labeling method for exploring nervous system diversity DOI Creative Commons
Marko Pende, Karim Vadiwala, Hannah Schmidbaur

et al.

Science Advances, Journal Year: 2020, Volume and Issue: 6(22)

Published: May 29, 2020

A tailored protocol combines pigment removal with clearing, facilitating labeling and whole-body imaging across phylogeny.

Language: Английский

Citations

82
Genomic architecture of a genetically assimilated seasonal color pattern DOI
Karin R. L. van der Burg, James J. Lewis, Benjamin J. Brack

et al.

Science, Journal Year: 2020, Volume and Issue: 370(6517), P. 721 - 725

Published: Nov. 6, 2020

Developmental plasticity allows genomes to encode multiple distinct phenotypes that can be differentially manifested in response environmental cues. Alternative plastic selected through a process called genetic assimilation, although the mechanisms are still poorly understood. We assimilated seasonal wing color phenotype naturally population of butterflies (Junonia coenia) and characterized three responsible genes. Endocrine assays chromatin accessibility conformation analyses showed transition coloration from an environmentally determined trait predominantly occurred selection for regulatory alleles downstream wing-patterning This mode evolution is likely favored by because it tissue- trait-specific tuning reaction norms without affecting core cue detection or transduction mechanisms.

Language: Английский

Citations

69
Highly Efficient Knockout of a Squid Pigmentation Gene DOI Creative Commons
Karen Crawford,

Juan Felipe Diaz Quiroz,

Kristen M. Koenig

et al.

Current Biology, Journal Year: 2020, Volume and Issue: 30(17), P. 3484 - 3490.e4

Published: July 30, 2020

Language: Английский

Citations

64
Synthetic, biological and optoelectronic properties of phenoxazine and its derivatives: a state of the art review DOI Open Access
Chandrita Sadhu, Amrit Krishna Mitra

Molecular Diversity, Journal Year: 2023, Volume and Issue: 28(2), P. 965 - 1007

Published: Feb. 9, 2023

Language: Английский

Citations

22
Whole-genome resequencing identifies candidate genes and SNPs in genomic regions associated with shell color selection in the Pacific oyster, Crassostrea gigas DOI
Yifei Zhang,

Chengxun Xu,

Qi Li

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 586, P. 740768 - 740768

Published: March 6, 2024

Language: Английский

Citations

7
Antioxidative Properties of Melanins and Ommochromes from Black Soldier Fly Hermetia illucens DOI Creative Commons
Н. А. Ушакова, А. Е. Донцов,

Н. Л. Сакина

et al.

Biomolecules, Journal Year: 2019, Volume and Issue: 9(9), P. 408 - 408

Published: Aug. 23, 2019

A comparative study of melanin and ommochrome-containing samples, isolated from the black soldier fly (BSF) by enzymatic hydrolysis, alkaline acid alcohol extraction or was carried out. Melanin both as a melanin-chitin complex water-soluble melanin. Acid hydrolysis followed delipidization yielded more concentrated sample, electron spin resonance (ESR) signal which 2.6 × 1018 spin/g. The ommochromes were extracted BSF eyes with methanol. antiradical activity melanins determined method quenching luminol chemiluminescence. It has been shown that increases its antioxidant properties. comparison in relation to photoinduced lipid peroxidation ESR characteristics native oxidized studied. is assumed H. illucens adult flies can be useful source natural pigments

Language: Английский

Citations

46
Creation of an albino squid line by CRISPR-Cas9 and its application for in vivo functional imaging of neural activity DOI
Namrata Ahuja, Ernie Hwaun, Judit R. Pungor

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(13), P. 2774 - 2783.e5

Published: June 20, 2023

Language: Английский

Citations

15
CRISPR-mediated knockout of cardinal and cinnabar eye pigmentation genes in the western tarnished plant bug DOI Creative Commons
Chan C. Heu,

Roni J. Gross,

Kevin P. Le

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: March 22, 2022

Abstract The western tarnished plant bug, Lygus hesperus , is a key hemipteran pest of numerous agricultural, horticultural, and industrial crops in the United States Mexico. A lack genetic tools L. hinders progress functional genomics developing innovative control methods such as gene drive. Here, using RNA interference (RNAi) against cardinal ( LhCd ), cinnabar LhCn white LhW we showed that knockdown was lethal to embryos, while or produced bright red eye phenotypes, contrast wild-type brown eyes. We further used CRISPR/Cas9 (clustered regularly interspaced palindromic repeats/CRISPR-associated) genome editing generate germline knockouts both (Card) (Cinn), producing separate strains characterized by mutant phenotypes. Although knockout strain Card exhibited gradual darkening eyes typical line later nymphal development, observed throughout all life stages Cinn, making it viable marker for tracking . These results provide evidence functions pigmentation genes are useful successful manipulation this insect.

Language: Английский

Citations

22
Efficient CRISPR/Cas9-mediated genome modification of the glassy-winged sharpshooter Homalodisca vitripennis (Germar) DOI Creative Commons
Inaiara de Souza Pacheco, Anna-Louise A. Doss,

Beatriz G. Vindiola

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: April 19, 2022

Abstract CRISPR/Cas9 technology enables the extension of genetic techniques into insect pests previously refractory to analysis. We report establishment analysis in glassy-winged sharpshooter (GWSS), Homalodisca vitripennis , which is a significant leafhopper pest agriculture California. use novel and simple approach embryo microinjection situ on host plant obtain high frequency mutagenesis, excess 55%, cinnabar white eye pigmentation loci. Through pair matings, we obtained 100% transmission w cn alleles G3 generation also established that both genes are located autosomes. Our wing phenotype revealed an unexpected discovery participation pteridine pigments wing-vein coloration, indicating role for these beyond color. used amplicon sequencing examine extent off-target mutagenesis adults arising from injected eggs, was found be negligible or non-existent. data show GWSS can easily developed as model system Hemiptera, enabling study traits contribute success invasive vectors pathogens. This will facilitate control strategies.

Language: Английский

Citations

22
Wearable Light Sensors Based on Unique Features of a Natural Biochrome DOI
Daniel J. Wilson, Francisco J. Martín‐Martínez, Leila F. Deravi

et al.

ACS Sensors, Journal Year: 2022, Volume and Issue: 7(2), P. 523 - 533

Published: Feb. 9, 2022

Overexposure to complete solar radiation (combined ultraviolet, visible, and infrared) is correlated with several harmful biological consequences including hyperpigmentation, skin cancer, eye damage, immune suppression. With limited effective therapeutic options available for these conditions, significant efforts have been directed toward promoting preventative habits. Recently, wearable radiometers emerged as practical tools managing personal exposure sunlight. However, designing simple inexpensive sensors that can measure energy across multiple spectral regions without incorporating electronic components remains challenging, largely due inherent limitations of photoresponsive indicators. In this work, we report the design, fabrication, characterization leverage an unexpected feature a natural biochrome, xanthommatin―its innate sensitivity both ultraviolet visible through near-infrared radiation. We found xanthommatin-based undergo shift from yellow red in presence This color change driven by intrinsic photoreduction molecule, which investigated using computational modeling supplemented radiation-driven formation complementary reducing agents. These are responsive dermatologically relevant doses erythemally weighted radiation, well cumulative high-energy used germicidal sterilization. incorporated miniature into pressure-activated microfluidic systems illustrate on-demand activation mountable form factor. When taken together, our findings encompass important advancement accessible, quantitative measurements UVC variety use cases.

Language: Английский

Citations

21