Overexpression of EZH2 is associated with clinicopathological parameters and poor prognosis in gliomas DOI
Can Peng, Wei Chen, Jun Yang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 30, 2024

Abstract Histone methyltransferase EZH2, primarily localized in the nucleus, mediates constitutive Polycomb repressive complex activity by trimethylating lysine 27 of histone H3 (H3K27me3), leading to gene silencing through canonical and noncanonical mechanisms, resulting transcriptional repression or activation. Its involvement is crucial cell growth, proliferation, differentiation, apoptosis, with its effects linked regulation various targets signaling pathways. Overexpression EZH2 alters expression function, thereby facilitating cancer progression. Recent research has identified potential prognostic role glioma patients. This study assesses clinicopathological significance value gliomas using available data. The mRNA levels tumor tissues normal were assessed timer2.0 data from CGCA TGCA. was determined Kaplan-Meier plotter. A total 147 clinical samples patients underwent immunohistochemistry analysis evaluate protein expression. Cox proportional hazards regression model survival curves employed assess relationship between expression, parameters, overall (OS). Across multiple cohorts, found be upregulated amplified tissues. In high-grade patients, significantly increased, higher correlated poorer OS, disease-specific (DSS), progression-free interval (PFI). Therefore, level may serve as a biomarker for

Language: Английский

Improving anti-oxidant stress treatment of subarachnoid hemorrhage through self-assembled nanoparticles of oleanolic acid DOI Creative Commons

Youdong Zhou,

Hengyu Wang, Xinyi Zhu

et al.

Drug Delivery, Journal Year: 2024, Volume and Issue: 31(1)

Published: Aug. 21, 2024

Subarachnoid hemorrhage (SAH) is a life-threatening acute hemorrhagic cerebrovascular disease, with early brain injury (EBI) being the main cause of high mortality and severe neurological dysfunction. Oxidative stress plays crucial role in pathogenesis EBI. In this study, we synthesized antioxidant nanoparticles based on self-assembled oleanolic acid (OA) using solvent volatilization method. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) techniques were employed to analyze understand self-assembly mechanism oleic (OA NPs). The TUNEL assay, Nissl staining, water content measurements conducted investigate impact OA NPs cortical neuronal injury. Additionally, Western blot analysis was performed NPs. result showed that exhibited spherical structure an average diameter 168 nm. application SAH has been found contribute reduction keap1 protein levels increase nuclear level Nrf2. As result, transcription proteins, including HO1 NQO1, triggered. activation pathway by ultimately leads decrease neuron damage improvement conclusion, successfully designed can efficiently target site SAH. These have demonstrated their potential as antioxidants for treatment SAH, offering significant clinical applications.

Language: Английский

Citations

2
Paclitaxel loaded Capmul MCM and tristearin based nanostructured lipid carriers (NLCs) for glioblastoma treatment: screening of formulation components by quality by design (QbD) approach DOI Creative Commons
Pooja Mittal,

M. Singla,

Smriti

et al.

Discover Nano, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 5, 2024

Paclitaxel (PTX), a naturally occurring diterpenoid isolated from Taxus brevifolia, is first-line drug for the treatment of glioblastoma; however, it suffers disadvantages poor water solubility and nonspecific biodistribution, which cause serious side effects in human body. The marketed formulation effects, such as allergic reactions, neutropenia, neuropathy, require safe effective formulations PTX. In present study, PTX was entrapped solid–liquid lipid mixture with aid surfactant using modified solvent evaporation technique. Higher entrapment impressive stability achieved by employing quality design-based strategies. Optimized levels numerical optimization technique each factor, that is, concentration (X1), (X2), amount organic (X3) were 0.3%, 0.76% & 8.3 ml respectively. resultant exhibited particle size 121.44 nm, efficiency 94.27%, zeta potential −20.21 mV unimodal distribution. A reduction % crystalline index 48 to 3.4% ensured amorphous form inside formulation, precludes fear leakage instability formulation. Cell line studies conducted on U87MG lines also suggested NLC paclitaxel are more than those pure summary, PTXNLC seem be superior alternative carrier system industry obtain higher excellent stability.

Language: Английский

Citations

1
Microglia Sing the Prelude of Neuroinflammation-Associated Depression DOI
Qingqing Li,

Ying Xie,

Jinyi Lin

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 13, 2024

Language: Английский

Citations

1
Active herbal ingredients and drug delivery design for tumor therapy: a review DOI
Jing Han,

Y. Shen,

Ruiying Cao

et al.

Chinese Journal of Natural Medicines, Journal Year: 2024, Volume and Issue: 22(12), P. 1134 - 1162

Published: Dec. 1, 2024

Language: Английский

Citations

1
High level of systemic immune inflammation index elevates delirium risk among patients in intensive care unit DOI Creative Commons
Feifei Xu, Shurong Zhang,

Yanyuan Zhang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 4, 2024

Evidence regarding the effect of systemic immune-inflammation index on delirium occurrence is limited. This study aimed to investigate association between SII and in intensive care unit (ICU) patients. Methods: Information was extracted from Medical Mart for Intensive Care-IV. Four logistic regression model established incorporated with subgroup analysis restricted cubic spline (RCS). The cutoff value acquired receiver operator characteristic curve (ROC), propensity score matching (PSM) utilized attenuate confounding effect. Survival evaluate relationship 30-day or 90-day all-cause mortality. Results: Among 7,518 participants, 1,685 cases occurred. Individuals highest quartile exhibited a heightened risk, significant multivariable-adjusted odds ratio (OR) 3.12(2.24,4.33). Tendency analysis, PSM together confirmed positive relationship. Results Cox displayed risk both mortality increased about 50% higher-SII group. Conclusion: Higher levels positively associated risk.

Language: Английский

Citations

0
Self-assembly of Natural Products of TCM and Bioactive Materials DOI Creative Commons

Dongxue Liu,

Miao Zhang,

Xinghua Sun

et al.

Pharmacological Research - Modern Chinese Medicine, Journal Year: 2024, Volume and Issue: unknown, P. 100559 - 100559

Published: Dec. 1, 2024

Language: Английский

Citations

0
Overexpression of EZH2 is associated with clinicopathological parameters and poor prognosis in gliomas DOI
Can Peng, Wei Chen, Jun Yang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 30, 2024

Abstract Histone methyltransferase EZH2, primarily localized in the nucleus, mediates constitutive Polycomb repressive complex activity by trimethylating lysine 27 of histone H3 (H3K27me3), leading to gene silencing through canonical and noncanonical mechanisms, resulting transcriptional repression or activation. Its involvement is crucial cell growth, proliferation, differentiation, apoptosis, with its effects linked regulation various targets signaling pathways. Overexpression EZH2 alters expression function, thereby facilitating cancer progression. Recent research has identified potential prognostic role glioma patients. This study assesses clinicopathological significance value gliomas using available data. The mRNA levels tumor tissues normal were assessed timer2.0 data from CGCA TGCA. was determined Kaplan-Meier plotter. A total 147 clinical samples patients underwent immunohistochemistry analysis evaluate protein expression. Cox proportional hazards regression model survival curves employed assess relationship between expression, parameters, overall (OS). Across multiple cohorts, found be upregulated amplified tissues. In high-grade patients, significantly increased, higher correlated poorer OS, disease-specific (DSS), progression-free interval (PFI). Therefore, level may serve as a biomarker for

Language: Английский

Citations

0