Acetylation of Steroidogenic Acute Regulatory Protein Sensitizes 17β-Estradiol Regulation in Hormone-Sensitive Breast Cancer Cells DOI Open Access
Pulak R. Manna,

Deborah Molehin,

Ahsen U. Ahmed

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8732 - 8732

Published: Aug. 10, 2024

An imbalance in estrogen signaling is a critical event breast tumorigenesis. The majority of cancers (BCs) are hormone-sensitive; they majorly express the receptor (ER+) and activated by 17β-estradiol (E2). steroidogenic acute regulatory protein (StAR) mediates rate-limiting step steroid biosynthesis. dysregulation epigenetic machinery, modulating E2 levels, primary occurrence for promoting StAR expression, concomitant with synthesis, was reported to be aberrantly high human mouse hormone-dependent BC cells compared their non-cancerous counterparts. However, mechanism action remains poorly understood. We discovered as an acetylated have identified number lysine (K) residues that putatively malignant non-malignant cells, using LC-MS/MS (liquid chromatography–tandem mass spectrometry), suggesting differently influence synthesis mammary tissue. treatment hormone-sensitive MCF7 variety histone deacetylase inhibitors (HDACIs), at therapeutically clinically relevant doses, few additional residues. Among total fourteen acetylomes undergoing acetylation deacetylation, K111 K253 were frequently recognized either endogenously or response HDACIs. Site-directed mutagenesis studies these two acetylomes, pertaining K111Q K253Q mimetic states, resulted increases levels ER+ triple negative MB-231 values seen StAR. Conversely, carrying K111R K253R deacetylation mutants diminished These findings provide novel mechanistic insights into intra-tumoral regulation elucidating functional importance this uncovered post-translational modification (PTM), involving events, underscoring potential therapeutic target BC.

Language: Английский

Harnessing the interplay of protein posttranslational modifications: Enhancing plant resilience to heavy metal toxicity DOI
Santosh Kumar,

Simpal Kumari,

Raghvendra Pratap Singh

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 295, P. 128112 - 128112

Published: Feb. 25, 2025

Language: Английский

Citations

0
Cardiac troponin DOI

Yumeng Gao,

Danchen Wang, Danni Mu

et al.

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 120344 - 120344

Published: May 1, 2025

Language: Английский

Citations

0
The Role of Proteomics in Identification of Key Proteins of Bacterial Cells with Focus on Probiotic Bacteria DOI Open Access
Miroslava Šťastná

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8564 - 8564

Published: Aug. 6, 2024

Probiotics can affect human health, keep the balance between beneficial and pathogenic bacteria, their colonizing abilities enable enhancement of epithelial barrier, preventing invasion pathogens. Health benefits probiotics were related to allergy, depression, eczema, cancer, obesity, inflammatory diseases, viral infections, immune regulation. Probiotic bacterial cells contain various proteins that function as effector molecules, explaining roles in probiotic actions is a key developing efficient targeted treatments for disorders. Systematic proteomic studies (probioproteomics) provide information about type involved, expression levels, pathological changes. Advanced methods with mass spectrometry instrumentation bioinformatics point out potential candidates next-generation are regulated under pharmaceutical frameworks. In addition, application proteomics other omics creates powerful tool expand our understanding diverse functionality. this review, strategies identification/quantitation bacteria overviewed. The types investigated by described, such intracellular proteins, surface secreted extracellular vesicles. Examples conditions which played crucial discussed.

Language: Английский

Citations

2
Acetylation of Steroidogenic Acute Regulatory Protein Sensitizes 17β-Estradiol Regulation in Hormone-Sensitive Breast Cancer Cells DOI Open Access
Pulak R. Manna,

Deborah Molehin,

Ahsen U. Ahmed

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8732 - 8732

Published: Aug. 10, 2024

An imbalance in estrogen signaling is a critical event breast tumorigenesis. The majority of cancers (BCs) are hormone-sensitive; they majorly express the receptor (ER+) and activated by 17β-estradiol (E2). steroidogenic acute regulatory protein (StAR) mediates rate-limiting step steroid biosynthesis. dysregulation epigenetic machinery, modulating E2 levels, primary occurrence for promoting StAR expression, concomitant with synthesis, was reported to be aberrantly high human mouse hormone-dependent BC cells compared their non-cancerous counterparts. However, mechanism action remains poorly understood. We discovered as an acetylated have identified number lysine (K) residues that putatively malignant non-malignant cells, using LC-MS/MS (liquid chromatography–tandem mass spectrometry), suggesting differently influence synthesis mammary tissue. treatment hormone-sensitive MCF7 variety histone deacetylase inhibitors (HDACIs), at therapeutically clinically relevant doses, few additional residues. Among total fourteen acetylomes undergoing acetylation deacetylation, K111 K253 were frequently recognized either endogenously or response HDACIs. Site-directed mutagenesis studies these two acetylomes, pertaining K111Q K253Q mimetic states, resulted increases levels ER+ triple negative MB-231 values seen StAR. Conversely, carrying K111R K253R deacetylation mutants diminished These findings provide novel mechanistic insights into intra-tumoral regulation elucidating functional importance this uncovered post-translational modification (PTM), involving events, underscoring potential therapeutic target BC.

Language: Английский

Citations

0