The alternative oxidase reconfigures the larval mitochondrial electron transport system to accelerate growth and development in Drosophila melanogaster DOI Open Access
Geovana S. Garcia, Murilo F Othonicar, A Campos

et al.

Published: May 9, 2025

Abstract The alternative oxidase (AOX) is naturally present in the mitochondrial electron transfer system (ETS) of many organisms but absent vertebrates and most insects. AOX oxidizes coenzyme Q reduces O2 H2O, partially replacing ETS cytochrome c segment alleviating oxidative stress caused by overload. As successfully demonstrated animal models, shows potential mitigating diseases. However, its non-proton-pumping nature may uncouple mitochondria, leading to excessive heat generation interference with normal metabolism physiology. Here we show that from tunicate Ciona intestinalis accelerates development Drosophila melanogaster, elevating larval biomass accumulation (primarily due increased fat), mobility food intake, without increasing body production. intensifies Leak respiration lowers phosphorylation efficiency through functional interactions glycerol-3-phosphate dehydrogenase (mGPDH). This associated complex I (CI)-driven supercomplex formation, higher cellular NAD+/NADH ratios, an enhanced flux central carbon metabolism. Chemical uncouplers rotenone confirm roles uncoupling CI AOX-expressing larvae. Thus, appears be promoting growth reinforcing proliferative metabolic program via intricate mechanism reconfigures ETS.

Language: Английский

Mitochondrial heat production: the elephant in the lab… DOI Creative Commons
Pierre Rustin, Howard T. Jacobs, Mügen Terzioglu

et al.

Trends in Biochemical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Spotlight on “Mitochondria operating at 50 °C?”: rethinking mitochondrial thermodynamics, bioenergetics and implications for mitochondrial medicine by Jacobs HT et al. 2024 DOI Creative Commons
Marvin Edeas, Volkmar Weissig

Journal of Mitochondria Plastids and Endosymbiosis, Journal Year: 2025, Volume and Issue: 3(1)

Published: April 12, 2025

Language: Английский

Citations

0
Tracing the evolutionary pathway: on the origin of mitochondria and eukaryogenesis DOI Creative Commons

J. Ernesto Bravo‐Arévalo

FEBS Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

The mito‐early hypothesis posits that mitochondrial integration was a key driver in the evolution of defining eukaryotic characteristics (DECs). Building on previous work identified endosymbiotic selective pressures as central to cell evolution, this study examines how gene transfer (EGT) and resulting genomic bioenergetic constraints shaped protein import systems. These systems were crucial for maintaining cellular function early eukaryotes facilitated their subsequent diversification. A primary focus is co‐evolution mechanisms endomembrane complexity. Specifically, I investigate necessity nuclear‐encoded drove adaptation bacterial secretion components, alongside innovations, refine translocation pathways. Beyond enabling expansion, endosymbiosis played fundamental role emergence compartmentalisation complexity LECA, driving organellar networks. By integrating genomic, structural phylogenetic evidence, aimed contribute framework, clarifying linked acquisition origin cells.

Language: Английский

Citations

0
The alternative oxidase reconfigures the larval mitochondrial electron transport system to accelerate growth and development in Drosophila melanogaster DOI Open Access
Geovana S. Garcia, Murilo F Othonicar, A Campos

et al.

Published: May 9, 2025

Abstract The alternative oxidase (AOX) is naturally present in the mitochondrial electron transfer system (ETS) of many organisms but absent vertebrates and most insects. AOX oxidizes coenzyme Q reduces O2 H2O, partially replacing ETS cytochrome c segment alleviating oxidative stress caused by overload. As successfully demonstrated animal models, shows potential mitigating diseases. However, its non-proton-pumping nature may uncouple mitochondria, leading to excessive heat generation interference with normal metabolism physiology. Here we show that from tunicate Ciona intestinalis accelerates development Drosophila melanogaster, elevating larval biomass accumulation (primarily due increased fat), mobility food intake, without increasing body production. intensifies Leak respiration lowers phosphorylation efficiency through functional interactions glycerol-3-phosphate dehydrogenase (mGPDH). This associated complex I (CI)-driven supercomplex formation, higher cellular NAD+/NADH ratios, an enhanced flux central carbon metabolism. Chemical uncouplers rotenone confirm roles uncoupling CI AOX-expressing larvae. Thus, appears be promoting growth reinforcing proliferative metabolic program via intricate mechanism reconfigures ETS.

Language: Английский

Citations

0
The alternative oxidase reconfigures the larval mitochondrial electron transport system to accelerate growth and development in Drosophila melanogaster DOI Open Access
Geovana S. Garcia, Murilo F Othonicar, A Campos

et al.

Published: May 9, 2025

Abstract The alternative oxidase (AOX) is naturally present in the mitochondrial electron transfer system (ETS) of many organisms but absent vertebrates and most insects. AOX oxidizes coenzyme Q reduces O2 H2O, partially replacing ETS cytochrome c segment alleviating oxidative stress caused by overload. As successfully demonstrated animal models, shows potential mitigating diseases. However, its non-proton-pumping nature may uncouple mitochondria, leading to excessive heat generation interference with normal metabolism physiology. Here we show that from tunicate Ciona intestinalis accelerates development Drosophila melanogaster, elevating larval biomass accumulation (primarily due increased fat), mobility food intake, without increasing body production. intensifies Leak respiration lowers phosphorylation efficiency through functional interactions glycerol-3-phosphate dehydrogenase (mGPDH). This associated complex I (CI)-driven supercomplex formation, higher cellular NAD+/NADH ratios, an enhanced flux central carbon metabolism. Chemical uncouplers rotenone confirm roles uncoupling CI AOX-expressing larvae. Thus, appears be promoting growth reinforcing proliferative metabolic program via intricate mechanism reconfigures ETS.

Language: Английский

Citations

0