Sex Differences in Antennal Transcriptome of Hyphantria cunea and Analysis of Odorant Receptor Expression Profiles DOI Open Access

Weichao Ma,

Yaning Li,

Lina Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 9070 - 9070

Published: Aug. 21, 2024

Insects rely on olfaction for mating, finding oviposition sites, and locating hosts. Hyphantria cunea is a serious pest that severely damages forests. Differential expression analysis of olfactory-related genes between males females the basis elucidating functions proteins in H. cunea. In this study, Illumina HiSeqTM 4000 high-throughput sequencing technology was used to perform transcriptome antennal tissues adult male female Functional annotation conducted using NR, Swiss-Prot, KOG, KEGG, GO databases, results showed contained 50,158 unigenes. identified 3923 were significantly differentially expressed antennae. A total 221 annotated, 96 sex-biased identified, including 13 odorant receptors (ORs), 48 binding (OBPs), 7 chemosensory (CSPs), 10 ionotropic (IRs), sensory neuron membrane (SNMPs), 2 gustatory (GRs), 6 odorant-degrading enzymes (ODEs), indicating there differences Quantitative real-time PCR verify levels 21 putative general receptor HcunOR4 HcunOR5 female-biased expression; HcunOR48, HcunOR49 HcunOR50 male-biased expression. The consistent with differential analysis. screening might provide theoretical functional characterization recognizing sex pheromones

Language: Английский

Sex Differences in Antennal Transcriptome of Hyphantria cunea and Analysis of Odorant Receptor Expression Profiles DOI Open Access

Weichao Ma,

Yaning Li,

Lina Yang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 9070 - 9070

Published: Aug. 21, 2024

Insects rely on olfaction for mating, finding oviposition sites, and locating hosts. Hyphantria cunea is a serious pest that severely damages forests. Differential expression analysis of olfactory-related genes between males females the basis elucidating functions proteins in H. cunea. In this study, Illumina HiSeqTM 4000 high-throughput sequencing technology was used to perform transcriptome antennal tissues adult male female Functional annotation conducted using NR, Swiss-Prot, KOG, KEGG, GO databases, results showed contained 50,158 unigenes. identified 3923 were significantly differentially expressed antennae. A total 221 annotated, 96 sex-biased identified, including 13 odorant receptors (ORs), 48 binding (OBPs), 7 chemosensory (CSPs), 10 ionotropic (IRs), sensory neuron membrane (SNMPs), 2 gustatory (GRs), 6 odorant-degrading enzymes (ODEs), indicating there differences Quantitative real-time PCR verify levels 21 putative general receptor HcunOR4 HcunOR5 female-biased expression; HcunOR48, HcunOR49 HcunOR50 male-biased expression. The consistent with differential analysis. screening might provide theoretical functional characterization recognizing sex pheromones

Language: Английский

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